LOCUS Exported 726 bp ds-DNA linear SYN 19-MAY-2021 DEFINITION Tissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoter. ACCESSION . VERSION . KEYWORDS pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 726) AUTHORS Di Donato V, De Santis F, Auer TO, Testa N, Sanchez-Iranzo H, Mercader N, Concordet JP, Del Bene F TITLE 2C-Cas9: a versatile tool for clonal analysis of gene function. JOURNAL Genome Res. 2016 Mar 8. PUBMED 26957310 REFERENCE 2 (bases 1 to 726) AUTHORS . TITLE Direct Submission JOURNAL Exported May 19, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..726 /organism="synthetic DNA construct" /mol_type="other DNA" promoter complement(13..30) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(59..78) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 113..132 /label=SV40pA-R /note="SV40 polyA, reverse primer" ORIGIN 1 agtaatctag aactatagtg agtcgtatta cgtagatcca gacatgataa gatacattga 61 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg 121 tgatgctatt gctttatttg taaccattat aagctgcaat aaaccttgta caaagtgggg 181 gatcctacgc ttacagctcc aaaactctac ggtaaaccta cataaactgc tggttttcaa 241 attttaaaga atttaagggt ttacaggttt actactacac agtgatttac tgacacatgt 301 aagtgtaaat gagttgaata agtaagtaag ccatatacca cacatgaaac acatacccag 361 aagtcactgg tatatatagc cgtcctccag actcccagct cgagacgatt agatctaatg 421 cgtctcgttt aagagctatg ctggaaacag catagcaagt ttaaataagg ctagtccgtt 481 atcaacttga aaaagtggca ccgagtcggt gctttttttc aatgatgaat taattcgagc 541 tccaccgcgg aagcttaaac aagaatctct agttttcttt cttgctttta cttttacttc 601 cttaatactc aagtacaatt ttaatggagt acttttttac ttttactcaa gtaagattct 661 agccagatac ttttactttt aattgagtaa aattttccct aagtacttgt actttcactt 721 gagtaa //