LOCUS       Exported                 915 bp ds-DNA     linear   SYN 20-MAY-2021
DEFINITION  pAAV vector to express oG (optimized Glycoprotein) in a 
            Cre-dependent manner.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-CAG-FLEX-oG-WPRE-SV40pA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Kim EJ, Jacobs MW, Ito-Cole T, Callaway EM
  TITLE     Improved Monosynaptic Neural Circuit Tracing Using Engineered Rabies
            Virus Glycoproteins
  JOURNAL   Cell Reports 2016
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 20, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    complement(66..99)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     protein_bind    complement(170..203)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
     regulatory      223..232
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattcga acctcttcga
       61 gggacataac ttcgtataat gtatgctata cgaagttatg ggtcgatggt gagatctgga
      121 ctagagggtc gatggtgatg cttggatcaa aataggaaga atcccaatga taacttcgta
      181 taaagtatcc tatacgaagt tatactagaa ttcggtaccg ccgccaccat ggtcccacag
      241 gctctcctct tcgtccctct gctcgtcttc ccactctgct ttggtaaatt ccctatctac
      301 actatccccg acaagctggg cccctggagt cctatcgata ttcaccatct gtcatgccct
      361 aacaatctcg tggtcgagga cgaaggctgt accaacctga gcggattctc ctacatggag
      421 ctgaaagtgg gctatatctc tgctattaag atgaacgggt tcacatgcac tggtgtggtc
      481 accgaggcag aaacctacac aaattttgtg ggatatgtca ccacaacttt caagagaaaa
      541 cactttcggc caacaccaga cgcctgtcgg gcagcttaca actggaagat ggctggcgac
      601 ccccgctatg aggaaagcct gcacaatcct tacccagact atcattggct gcgcactgtg
      661 aagaccacaa aagagagcct ggtcatcatt tccccatctg tcgccgacct ggacccctac
      721 gaccgatctc tccacagtcc agtgtttccc ggcggaaact gctccggagt ggccgtcagc
      781 tccacttact gttccaccaa ccatgattat acaatctgga tgcctgagaa tccaaggctg
      841 gggatgtcct gcgacatctt caccaacagc aggggcaaga gggcctcaaa aggcagcgag
      901 acttgtggat ttgtg
//
