LOCUS Exported 883 bp ds-DNA linear SYN 31-MAY-2021 DEFINITION The CRISPR construct targets near the LoxP sites in Rosa-pCA-loxP-mTdtomato-loxP-mEGFP mice.. ACCESSION . VERSION . KEYWORDS CRISPRmTmG2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 883) AUTHORS Wang S, Sengel C, Emerson MM, Cepko CL TITLE A gene regulatory network controls the binary fate decision of rod and bipolar cells in the vertebrate retina. JOURNAL Dev Cell. 2014 Sep 8;30(5):513-27. doi: 10.1016/j.devcel.2014.07.018. Epub 2014 Aug 21. PUBMED 25155555 REFERENCE 2 (bases 1 to 883) AUTHORS . TITLE Direct Submission JOURNAL Exported May 31, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..883 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 124..364 /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" primer_bind 124..144 /label=hU6-F /note="Human U6 promoter, forward primer" primer_bind 295..314 /label=LKO.1 5' /note="Human U6 promoter, forward primer" misc_RNA 394..469 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" enhancer 566..851 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer; contains an 18-bp deletion relative to the standard CMV enhancer" ORIGIN 1 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 61 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 121 tgtgagggcc tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga 181 gagataattg gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt 241 agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat 301 catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa 361 ggacgaaaca ccgcgaagtt atattaaggg ttcgttttag agctagaaat agcaagttaa 421 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttgtttt 481 agagctagaa atagcaagtt aaaataaggc tagtccgttt ttagcgcgtg cgccaattct 541 gcagacaaat ggctctagag gtacccgtta cataacttac ggtaaatggc ccgcctggct 601 gaccgcccaa cgacccccgc ccattgacgt caatagtaac gccaataggg actttccatt 661 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc 721 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattgtg 781 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 841 ctattaccat ggtcgaggtg agccccacgt tctgcttcac tct //