LOCUS Exported 10960 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Translation reporter. ACCESSION . VERSION . KEYWORDS pcDNA4TO-24xGCN4_v4-kif18b-24xPP7 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10960) AUTHORS Yan X, Hoek TA, Vale RD, Tanenbaum ME TITLE Dynamics of Translation of Single mRNA Molecules In Vivo. JOURNAL Cell. 2016 May 5;165(4):976-89. doi: 10.1016/j.cell.2016.04.034. PUBMED 27153498 REFERENCE 2 (bases 1 to 10960) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10960 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(44..63) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 769..789 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" protein_bind 820..838 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 841..859 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" primer_bind 869..893 /label=LNCX /note="Human CMV promoter, forward primer" regulatory 984..993 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1059..1115 /codon_start=1 /product="GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v4 /translation="EELLSKNYHLENEVARLKK" CDS 1131..1187 /codon_start=1 /product="GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v4 /translation="EELLSKNYHLENEVARLKK" CDS 1203..1259 /codon_start=1 /product="GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v4 /translation="EELLSKNYHLENEVARLKK" CDS 1275..1331 /codon_start=1 /product="GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v4 /translation="EELLSKNYHLENEVARLKK" CDS 1347..1403 /codon_start=1 /product="GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v4 /translation="EELLSKNYHLENEVARLKK" CDS 1419..1475 /codon_start=1 /product="GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v4 /translation="EELLSKNYHLENEVARLKK" CDS 1491..1547 /codon_start=1 /product="GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v4 /translation="EELLSKNYHLENEVARLKK" CDS 1563..1619 /codon_start=1 /product="GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v4 /translation="EELLSKNYHLENEVARLKK" CDS 1635..1691 /codon_start=1 /product="GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v4 /translation="EELLSKNYHLENEVARLKK" CDS 1707..1763 /codon_start=1 /product="GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v4 /translation="EELLSKNYHLENEVARLKK" CDS 1779..1835 /codon_start=1 /product="GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v4 /translation="EELLSKNYHLENEVARLKK" CDS 1851..1907 /codon_start=1 /product="GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v4 /translation="EELLSKNYHLENEVARLKK" CDS 1923..1979 /codon_start=1 /product="GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v4 /translation="EELLSKNYHLENEVARLKK" CDS 1995..2051 /codon_start=1 /product="GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v4 /translation="EELLSKNYHLENEVARLKK" CDS 2067..2123 /codon_start=1 /product="GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v4 /translation="EELLSKNYHLENEVARLKK" CDS 2139..2195 /codon_start=1 /product="GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v4 /translation="EELLSKNYHLENEVARLKK" CDS 2211..2267 /codon_start=1 /product="GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v4 /translation="EELLSKNYHLENEVARLKK" CDS 2283..2339 /codon_start=1 /product="GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v4 /translation="EELLSKNYHLENEVARLKK" CDS 2355..2411 /codon_start=1 /product="GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v4 /translation="EELLSKNYHLENEVARLKK" CDS 2427..2483 /codon_start=1 /product="GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v4 /translation="EELLSKNYHLENEVARLKK" CDS 2499..2555 /codon_start=1 /product="GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v4 /translation="EELLSKNYHLENEVARLKK" CDS 2571..2627 /codon_start=1 /product="GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v4 /translation="EELLSKNYHLENEVARLKK" CDS 2715..2771 /codon_start=1 /product="GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v4 /translation="EELLSKNYHLENEVARLKK" primer_bind complement(6971..6988) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 6977..7201 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 7247..7675 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7334..7353) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7544..7565 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(7684..7704) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 7689..8018 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 7869..8004 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7931..7950 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 8066..8113 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 8132..8506 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 8636..8757 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8673..8692) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8727..8746 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(8806..8822) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8806..8822) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8819..8841) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8830..8846 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8854..8884) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8899..8920 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(9037..9054) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(9208..9793) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(9288..9307) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9964..10824) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10587..10606 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10825..10929) /gene="bla" /label=AmpR promoter ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc 841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga 901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga 961 ctctagcgtt taaacttaag cttgccacca tgggcgccat catacacgcg gccgcaatca 1021 tcggtccggg tggatctgga ggtggaggtt ctggaggaga agaacttttg agcaagaatt 1081 atcatcttga gaacgaagtg gctcgtctta agaaaggttc tggcagtgga gaagaactgc 1141 tttcaaagaa ttaccacctg gaaaatgagg tagctagact gaaaaagggg agcggaagtg 1201 gggaggagtt gctgagcaaa aattatcatt tggagaacga agtagcacga ctaaagaaag 1261 ggtccggatc gggtgaggag ttactctcga aaaattatca tctcgaaaac gaagtggctc 1321 ggctaaaaaa gggcagtggt tctggagaag agctattatc taaaaactac cacctcgaaa 1381 atgaggtggc acgcttaaaa aagggaagtg gcagtggtga agagctacta tccaagaatt 1441 atcatcttga gaacgaggta gcgcgtttga agaagggttc cggctcagga gaggaactgc 1501 tctcgaagaa ctatcatctt gaaaatgagg tcgctcgatt aaaaaaggga tcgggcagtg 1561 gtgaggaact actttcaaag aattaccacc tcgaaaacga agtagctcga ttaaagaaag 1621 gttcagggtc gggtgaagaa ttactgagta aaaattatca tctggaaaat gaggtagcga 1681 gactaaaaaa ggggagtggt tctggcgaag agttgctatc gaaaaattat catcttgaga 1741 acgaagttgc taggctcaaa aagggctcag gctcaggcga ggagttgctc tcgaaaaact 1801 accacttgga aaatgaggtc gcgaggttga aaaaggggag cgggtcgggc gaggagttat 1861 tgagcaaaaa ctatcattta gagaacgaag tcgcgcgctt aaagaaaggc tcgggctcgg 1921 gcgaagaact cttatcgaag aactaccacc tcgaaaatga ggtcgccagg ttgaaaaagg 1981 gcagtggcag cggggaggaa ctcttgagca agaactacca cttggagaat gaggtcgcga 2041 gattgaagaa agggtcgggg agcggcgagg aattgctcag caagaattat catttggaga 2101 acgaagtcgc caggctcaag aaaggctcgg ggtcggggga ggaattgttg agtaaaaact 2161 accacttgga aaatgaagtc gccaggctca aaaaagggag tgggagcggc gaagagttat 2221 tgagcaaaaa ttaccacttg gagaacgaag tggcaaggct caagaaaggg agcggcagcg 2281 gggaggagct cttatcgaag aactaccact tagagaatga agtcgcccgc ttgaagaaag 2341 gctcggggag cggggaagag ctcttgagca agaactacca cttggaaaat gaggtggcgc 2401 gcttgaagaa agggagcggg agcggggaag agttactatc taagaattat catctcgaga 2461 acgaggtggc tcgactaaag aagggctccg gcagtgggga ggaactcctg tcgaagaact 2521 atcatcttga aaatgaggtt gcaagactta aaaaggggtc cggatcaggt gaggaactac 2581 tcagtaagaa ttaccacctg gaaaacgaag ttgcacgttt gaagaaagga tcaggatcag 2641 gcgaagaact gctctcaaaa gattatcatt tggaaaatga ggttgcacgt ttaaaaaagg 2701 gaagtggcag tggtgaggaa cttctgtcga aaaattatca tctcgagaat gaagtagccc 2761 gacttaaaaa gggttctggc tcgggtcagc ggccgcaccg gtcagcagtg gaggacagca 2821 cgctgcaagt agtggtacgg gtgcggcccc ccacccctcg ggagctggac agtcagcggc 2881 ggccagtggt tcaggtggtg gacgagcggg tgctggtgtt taaccctgag gagcccgatg 2941 gagggttccc tggcctgaaa tggggtggca cccatgatgg ccccaagaag aagggcaaag 3001 acctgacgtt tgtctttgac cgggtctttg gcgaggcggc cacccaacag gacgtgttcc 3061 agcacaccac gcacagcgtc ctggacagct tcctccaggg ctacaactgc tcagtgtttg 3121 cctacggggc caccggggct gggaagacac acaccatgct gggaagggag ggggaccccg 3181 gcatcatgta cctgaccacc gtggaactgt acaggcgcct ggaggcccgc cagcaggaga 3241 agcacttcga ggtgctcatc agctaccagg aggtgtataa tgaacagatc catgacctcc 3301 tggagcccaa ggggcccctt gccatccgcg aggaccccga caagggggtg gtggtgcaag 3361 gactttcttt ccaccagcca gcctcagccg agcagctgct ggagatactg accaggggga 3421 accgtaaccg cacgcagcac cccactgatg ccaacgcgac ttcctcccgc tcccatgcca 3481 tcttccagat ctttgtgaag cagcaggacc gggttccagg actgacccag gctgtccagg 3541 tggccaagat gagcctgatt gacctggctg gctcagagcg ggcatccagc acccatgcga 3601 agggggagcg gctgcgggag ggggccaaca tcaaccgctc tctgctggcg ctcatcaacg 3661 tcctcaatgc cttggccgat gcaaagggcc gcaagaccgc tgtgccctac gcggacagcg 3721 cactgacccg cctgctcaaa gactccctcg ggggcaactg ccgcacagtg atgatcgctg 3781 ccatcagccc ctccagcctg acctacgagg acacgtataa taccctcaaa tatgccgacc 3841 gggccaagga gatcaggctc tcgctgaaga gcaatgtgac cagcctggac tgtcacatca 3901 gccagtatgc taccatctgc caacagctcc aggctgaggt agccgctctg aggaagaagc 3961 tccaagtgta tgagggggga ggccagcccc caccacagga cctcccagga tctcccaagt 4021 cgggaccacc accagaacac cttcccagct cccccttgcc accccaccct cccagccagc 4081 cctgcacccc agagctccct gcagggccta gagcccttca agaggagagt ctggggatgg 4141 aggcccaggt ggagagggcc atggaaggga actcttcaga ccaggagcag tccccagagg 4201 atgaggatga aggcccagct gaggaggttc caacccagat gccagagcag aaccccacac 4261 atgcactgcc agagtcccct cgcctgaccc tgcagcccaa gccagtcgtg ggccacttct 4321 cagcacggga actggatggg gaccgttcta agcggttggc cctaaaggtg ctgtgcgttg 4381 cccagcggca gtactccctg ctccaagcag ccaacctcct gacgcccgac atgatcacag 4441 agtttgagac cctacagcag ctggtgcaag aggaaaaaat tgagcctggg gcagaggcct 4501 tgaggacttc aggcctggcc aggggggcac ctctggctca ggagctgtgt tcagagtcaa 4561 agcctccagg atacactggc cctgtgaccc ggactatggc gaggcgactg agtggccccc 4621 tgcacaccct gggaatcccg cctggaccca actgcacccc agcccagggg tcccgatggc 4681 ccatggagaa gaagaggagg agaccaagcg ccttggaggc agacagtccc atggccccaa 4741 agcggggcac caagcgccag cgccagtcct tcctgccctg cctaaggaga gggtctctgc 4801 ctgacaccca accttcacag gggcccagca cccccaaagg agaaagggcc tcctccccct 4861 gccattcccc tcgcgtttgc ccagccacag tcatcaaaag ccgggtgccc ctgggccctt 4921 ccgccatgca gaactgctcc accccgctgg ctctgcccac tcgagacctc aatgccacct 4981 ttgatctctc tgaggagcct ccctcaaagc ccagtttcca tgaatgcatt ggctgggaca 5041 aaatacccca ggagctgagc aggctggacc agcccttcat ccccagggca cctgtgcccc 5101 tgttcaccat gaagggcccc aagccaacat cttccctccc tgggacctct gcctgcaaga 5161 agaagcgcgt tgcgagttcc tcagtctccc atggccgcag ccgcatcgcc cgcctcccca 5221 gcagcacttt gaagaggcca gctgggcccc ttgtactccc agagctgccc ttgagtcccc 5281 tgtgccctag caaccggagg aatggaaagg acctcatcag ggtggggaga gcactctcag 5341 cagggaacgg cgtcaccaag gtgtccgata tctaacgaca cgattcgaag atcgcatcgc 5401 tagcccggtt gtacaggtac cggatcctaa ggtacctaat tgcctagaaa ggagcagacg 5461 atatggcgtc gctccctgca ggtcgactct agaaaccagc agagcatatg ggctcgctgg 5521 ctgcagtatt cccgggttca ttagatccta aggtacctaa ttgcctagaa aggagcagac 5581 gatatggcgt cgctccctgc aggtcgactc tagaaaccag cagagcatat gggctcgctg 5641 gctgcagtat tcccgggttc attagatcct aaggtaccta attgcctaga aaggagcaga 5701 cgatatggcg tcgctccctg caggtcgact ctagaaacca gcagagcata tgggctcgct 5761 ggctgcagta ttcccgggtt cattagatcc taaggtacct aattgcctag aaaggagcag 5821 acgatatggc gtcgctccct gcaggtcgac tctagaaacc agcagagcat atgggctcgc 5881 tggctgcagt attcccgggt tcattagatc ctaaggtacc taattgccta gaaaggagca 5941 gacgatatgg cgtcgctccc tgcaggtcga ctctagaaac cagcagagca tatgggctcg 6001 ctggctgcag tattcccggg ttcattagat cctaaggtac ctaattgcct agaaaggagc 6061 agacgatatg gcgtcgctcc ctgcaggtcg actctagaaa ccagcagagc atatgggctc 6121 gctggctgca gtattcccgg gttcattaga tcctaaggta cctaattgcc tagaaaggag 6181 cagacgatat ggcgtcgctc cctgcaggtc gactctagaa accagcagag catatgggct 6241 cgctggctgc agtattcccg ggttcattag atcctaaggt acctaattgc ctagaaagga 6301 gcagacgata tggcgtcgct ccctgcaggt cgactctaga aaccagcaga gcatatgggc 6361 tcgctggctg cagtattccc gggttcatta gatcctaagg tacctaattg cctagaaagg 6421 agcagacgat atggcgtcgc tccctgcagg tcgactctag aaaccagcag agcatatggg 6481 ctcgctggct gcagtattcc cgggttcatt agatcctaag gtacctaatt gcctagaaag 6541 gagcagacga tatggcgtcg ctccctgcag gtcgactcta gaaaccagca gagcatatgg 6601 gctcgctggc tgcagtattc ccgggttcat tagatcctaa ggtacctaat tgcctagaaa 6661 ggagcagacg atatggcgtc gctccctgca ggtcgactct agaaaccagc agagcatatg 6721 ggctcgctgg ctgcagtatt cccgggttca ttagatccta aggtacctaa ttgcctagaa 6781 aggagcagac gatatggcgt cgctccctgc aggtcgactc tagaaaccag cagagcatat 6841 gggctcgctg gctgcagtat tcccgggttc attagatctc gcgaagggcg aattcacgag 6901 gcgcgcccgg atcgcgatcg caatcttaat taactcgagt ctagagggcc cgtttaaacc 6961 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc 7021 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa 7081 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac 7141 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg 7201 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc 7261 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc 7321 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt 7381 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac 7441 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag 7501 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa 7561 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg 7621 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc 7681 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta 7741 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag 7801 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa 7861 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 7921 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt 7981 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat 8041 ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat 8101 agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt 8161 gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc 8221 ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat 8281 cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg 8341 cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc 8401 cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga 8461 cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga 8521 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc 8581 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt 8641 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 8701 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 8761 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc 8821 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 8881 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 8941 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 9001 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 9061 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 9121 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 9181 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 9241 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 9301 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 9361 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 9421 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 9481 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 9541 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 9601 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg 9661 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 9721 caaacaaacc accgctggta gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa 9781 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 9841 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 9901 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 9961 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 10021 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 10081 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 10141 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 10201 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 10261 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 10321 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 10381 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 10441 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 10501 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 10561 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 10621 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 10681 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 10741 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 10801 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 10861 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 10921 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc //