LOCUS       Exported                6056 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Express c-Myc-tagged human ASC in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA3-Myc-ASC
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6056)
  AUTHORS   Bryan NB, Dorfleutner A, Rojanasakul Y, Stehlik C
  TITLE     Activation of inflammasomes requires intracellular redistribution of
            the apoptotic speck-like protein containing a caspase recruitment 
            domain.
  JOURNAL   J Immunol. 2009 Mar 1;182(5):3173-82. doi: 10.4049/jimmunol.0802367.
  PUBMED    19234215
REFERENCE   2  (bases 1 to 6056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6056
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             909..938
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(1608..1626)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1609..1626)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1646..1663)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1652..1876
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1922..2350
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2009..2028)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2219..2240
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2359..2379)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2364..2694
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2545..2680
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2607..2626
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             2761..3555
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2815..2834)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3425..3444
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3729..3850
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3766..3785)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3820..3839
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3899..3915)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3899..3915)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3912..3934)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3923..3939
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3947..3977)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3992..4013
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4130..4147)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4301..4889)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4381..4400)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5060..5920)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5683..5702
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5921..6025)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttcgatcg
      901 tcgacatgga acagaaactc atctctgaag aggatctgtc tagaggatcc gaattcgatc
      961 ctggagccat ggggcgcgcg cgcgacgcca tcctggatgc gctggagaac ctgaccgccg
     1021 aggagctcaa gaagttcaag ctgaagctgc tgtcggtgcc gctgcgcgag ggctacgggc
     1081 gcatcccgcg gggcgcgctg ctgtccatgg acgccttgga cctcaccgac aagctggtca
     1141 gcttctacct ggagacctac ggcgccgagc tcaccgctaa cgtgctgcgc gacatgggcc
     1201 tgcaggagat ggccgggcag ctgcaggcgg ccacgcacca gggctctgga gccgcgccag
     1261 ctgggatcca ggcccctcct cagtcggcag ccaagccagg cctgcacttt atagaccagc
     1321 accgggctgc gcttatcgcg agggtcacaa acgttgagtg gctgctggat gctctgtacg
     1381 ggaaggtcct gacggatgag cagtaccagg cagtgcgggc cgagcccacc aacccaagca
     1441 agatgcggaa gctcttcagt ttcacaccag cctggaactg gacctgcaag gacttgctcc
     1501 tccaggccct aagggagtcc cagtcctacc tggtggagga cctggagcgg agctgaggct
     1561 ccttcccagc aacactccgg ctcgagcatg catctagagg gccctattct atagtgtcac
     1621 ctaaatgcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     1681 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     1741 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     1801 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     1861 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     1921 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     1981 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     2041 acgttcgccg gctttccccg tcaagctcta aatcggggca tccctttagg gttccgattt
     2101 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     2161 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     2221 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     2281 taagggattt tggggatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     2341 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     2401 caggcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     2461 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     2521 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     2581 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     2641 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     2701 ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc
     2761 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     2821 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     2881 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     2941 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     3001 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     3061 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     3121 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     3181 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     3241 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     3301 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     3361 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     3421 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     3481 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     3541 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     3601 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     3661 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     3721 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     3781 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     3841 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     3901 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     3961 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     4021 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     4081 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     4141 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     4201 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     4261 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     4321 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     4381 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     4441 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct
     4501 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     4561 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     4621 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     4681 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     4741 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     4801 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     4861 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     4921 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     4981 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     5041 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     5101 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     5161 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     5221 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     5281 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     5341 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     5401 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     5461 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     5521 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     5581 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     5641 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     5701 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     5761 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     5821 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     5881 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     5941 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     6001 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc
//
