LOCUS       Exported                 865 bp ds-DNA     linear   SYN 25-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCAG_PSD95.FingR-eGFP-CCR5TC
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Gross GG, Junge JA, Mora RJ, Kwon HB, Olson CA, Takahashi TT, Liman 
            ER, Ellis-Davies GC, McGee AW, Sabatini BL, Roberts RW, Arnold DB
  TITLE     Recombinant probes for visualizing endogenous synaptic proteins in 
            living neurons.
  JOURNAL   Neuron. 2013 Jun 19;78(6):971-85. doi: 10.1016/j.neuron.2013.04.017.
  PUBMED    23791193
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 25, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             282..305
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             330..845
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIE"
     primer_bind     complement(372..393)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(633..652)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ccgggattac aatgctcgaa gtcaaggaag catcaccaac cagcatccag atcagctggg
       61 tgctccactt gcgccacgtt cgctactacc gcatcaccta cggtgaaact ggtggcaata
      121 gccctgtcca ggaattcacc gtgcctggca gcaagtccac tgctaccatc agcggcctga
      181 aacctggtgt cgactatacc atcacggtgt acgccgtcac gatcttcagc gcctaccgct
      241 ccgcctggcc gccgatctcc atcaactacc gcaccggaac cgattacaag gatgacgacg
      301 ataagggtag cggctccagt agatctgggg tgagcaaggg cgaggagctg ttcaccgggg
      361 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
      421 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
      481 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
      541 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
      601 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
      661 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
      721 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
      781 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
      841 tcgaggacgg cagcgtgcag ctcgc
//
