LOCUS       Exported                5507 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Can be used to drive expression specifically in zebrafish motor 
            neurons.
ACCESSION   .
VERSION     .
KEYWORDS    p5E--3mnx1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5507)
  AUTHORS   Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan 
            A, Chow S, Gwee SS, Stoddart JJ, Nicholson G, Chung R, Cole NJ
  TITLE     A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous 
            System and Neurodegenerative Disease.
  JOURNAL   Zebrafish. 2016 Sep 15.
  PUBMED    27631880
REFERENCE   2  (bases 1 to 5507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5507
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      268..295
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      387..473
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    3552..3676
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3748..3767)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3749..3767)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3772..3788)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3772..3788)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3890..3909)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3901..4710
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3968..3987)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4857..5445
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5346..5365
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttgct gaggaaaacg ctttcgacta cagaaggact
      721 ttgtattatg caatgttttc aaaaaaatat cttcatattc agtgtgggag ataataagaa
      781 aggaaaaagc atgaagcact gaatcggtgt cctttaatga cttttatagt tgggaacaat
      841 tagccgttct ccacaaatta aattgataaa aagattacga gatccaaatg cgtttaaaag
      901 ctgtggtacg cacggcccat ttgtgcaatt atgatcgaaa atatttattg caataaaaga
      961 gacagtgcag gtcccgttaa attggctttt cataatgaac acatttaaca tggataatta
     1021 atatataaac tatttcgatt tgtcacgatg atatgtgttg cacttaattg tgaaagtaat
     1081 taccgagcga atgaacatct ttgaggagag tccctggttt caggccctcg tatcatcggg
     1141 tgcaataata attttactct ctcctgatgt gatgaaaaag agccataaat ctctcagatc
     1201 gctgcaggtc cacggcttca tatttcaccg ctgctttaat agaaatattc atgcgatccc
     1261 ttaatgaaat taaaactgag aagcggacaa agcggcccta ggctatgtgc aacgtgtaaa
     1321 gacacaagag catttgattg ggaaaatgtt gcatccttca aatatgagga tgttgttgca
     1381 ctgtaaacca cgtaaaacaa tttttttctt acagtccagt attggccaca cttgataatt
     1441 gttacatttg ttatcaatta cacgcgaggc tcattgaatg tcatttcaga attggtcagt
     1501 gcatgcgtca ttacggcacg cttttattct catcctttcg gccggtgact tgttgatttc
     1561 cctcgagcaa ataaacgcat gcgaccagtc tctcgagagt ggttagctga tgaattgagg
     1621 aaaactaatc cgctttattg gtagacaggt taagggcaac agagtgtcaa taattctctc
     1681 cctgacctct ttctccatta gcttaaagag gcttataaga ggaacagacc ccacaacatg
     1741 acaccgatcc tccacatcac catctcacat gtagccaacc taggcttgga aaatcatttc
     1801 gatttataaa gctcaagatc caacatttgc caaaaattca tgaaaacagg gcacaaatag
     1861 ggcatcattt tctcacaaag tattatatag gcctaattag atagatagat agatagatag
     1921 atagatagat agatagatag atagatagat agatagatag atagatagat agatagatag
     1981 atagatagat agatagatag atagatagat agatagatag atagatagat agatagatag
     2041 atagatagat agatagacag atagatagat agaaaaaaaa caagattttg cttgtgattc
     2101 attatgcata ataatttgcc tatgtttatt aataaacatc gtgttaggat tataaatgtg
     2161 ttgtgatgaa tgtgattaaa tgtttaaata tttactgaca gttgttagcc tatatttaca
     2221 gatgtaaaaa atctacgtct aattagtatg tgtttcttaa atctataata aggaaaggct
     2281 tattaagttt atctgtacaa tttagaacaa ttttacgaat ttatccttta gagaaaaaaa
     2341 atgtaataac accatattta aatatcctgt tgagaaatta ttaaatcaaa tttttacatt
     2401 ctaagagcaa ggttaaatat tgagtgaaag gttgaattgt ttttaagaca gttgttttat
     2461 ttttttaagc atattgtggt gggtgagttt tcaaagattt acacatttca aacaatttat
     2521 taaaacaata tttgttgcat aaaacactga tttggtcagt tttaccactg actttaactt
     2581 atttatttaa ttaattatat ttctattgtt cattgttatt aaattttgcc tacctaatgc
     2641 tctctaacaa ataaggaata acttgaatat ttcctctgcg caaaatgttc atcgtaatgt
     2701 aaaagatccg tttgacattt aaaatggttc tcttctcata aagatagtaa ttcttttaag
     2761 aactgtcctc taaaaggttc tttggggaaa caaaaaaata tacgtgtaca acatcaatgt
     2821 gaaaatcaaa tagttttttt tattatcgca ggctgttcag ataaaaaaat acaattgaat
     2881 aaaaaagtat ggacattaaa atggtgtcat agcaaaatga caaatgcgtg tatagactat
     2941 ggttttttgt taattaattt gatgaaataa aaaaaaatta tacaaatgaa aaaaatgtca
     3001 tgcctctaca tctatgcgaa taaaatgcat ggctgttttt tttttttttt tttttgttca
     3061 aacaaactat ttcgccaaaa taatattttc gagttaacgt gctttatcaa tcttcgttta
     3121 tattaagatt ataggtcggt gtttaaataa tgaaatcttg ataactttat aaatgtttca
     3181 tgtttcaaag ttgcatgtta ataattgccc tttagactct acaaatctgg aattattcat
     3241 aaattaagta gatgtaaaat gtatgtgtgc atgttgtgtt tcccacttaa aaagctttca
     3301 ttttatggga gaacatactt atgacaactc ttgaacttct acaagcacac cgtgaacact
     3361 ggaatcactg tccaatcgga aatgcacaag aatgacgtga atgaccaatc cactccctct
     3421 cgggcagcag ttgacaagtg gacttctggc ttgcacacct tggccaagat cataacttta
     3481 cgaagaggag cggtgacact cctgacagcc ttttaaagtt acgaacgacg cgttaatctg
     3541 tttgtgagtt ggcaagtttg tacaaaaaag ttgaacgaga aacgtaaaat gatataaata
     3601 tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac
     3661 atatgcagtc actatgaacc aactacttag atggtattag tgacctgtag aattcgagct
     3721 ctagagctgc agggcggccg cgatatcccc tatagtgagt cgtattacat ggtcatagct
     3781 gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa
     3841 aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt
     3901 atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat
     3961 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc
     4021 ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc
     4081 aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg
     4141 accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc
     4201 ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat
     4261 gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac
     4321 agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat
     4381 gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg
     4441 cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat
     4501 aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc
     4561 gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca
     4621 ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag
     4681 tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg
     4741 cagagcatta cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg
     4801 agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     4861 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     4921 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     4981 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     5041 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     5101 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     5161 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     5221 accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga
     5281 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     5341 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     5401 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     5461 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgtt
//
