LOCUS Exported 5972 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Can be used to drive pan neuronal expression in zebrafish.. ACCESSION . VERSION . KEYWORDS p5E-elavl3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5972) AUTHORS Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan A, Chow S, Gwee SS, Stoddart JJ, Nicholson G, Chung R, Cole NJ TITLE A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous System and Neurodegenerative Disease. JOURNAL Zebrafish. 2016 Sep 15. PUBMED 27631880 REFERENCE 2 (bases 1 to 5972) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5972 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 593..688 /gene="mutant version of attL" /label=attL4 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 690..708 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(3946..3962) /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3946..3962) /label=pBluescriptSK /note="For pBluescript vector" promoter complement(3999..4017) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" protein_bind 4017..4141 /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter complement(4214..4232) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(4237..4253) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4237..4253) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(4355..4374) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 4366..5175 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(4433..4452) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 5322..5910 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5811..5830 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg 601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt 661 ataatgccaa ctttgtatag aaaagttggt aatacgactc actatagggc gaattgggta 721 ccgggccccc cctcgagctc aagcttcgaa ttcactaatt tgaatttaaa tgcattatct 781 ttctattcct aaagaccttg ggtgaccaaa atcttatttt aataaataaa actgtttatt 841 aaaacttttt tgtttcaaag aaccatatgt atagtgaaat ttataaaaat atcaattttt 901 aaaaagctgg tgtactcatt tatgttatga actctaaaac catatactga ctgcaagtga 961 tgatgtatag agtgatgttt acgagtaaac atatttagtt gtatacatcc tactgagcac 1021 attttgatgt atgaaataac attacaagct ttatccaaat taagccattt taaaacactg 1081 ccaattgaaa atacaaatcc tggaaaaaat cgtctttagc gcagtcattt gagccatcct 1141 aatccgttac ctcagaccat aataagaagg gataacacta gctgtagcaa tggaacacat 1201 ctgtttcaca caatcatatc tcctgcgccg gtgctaagca gattcagcgt gatcataaca 1261 tgctttccac tcataaatgt aaatttacaa tttgcacatg taaaacagac acttttgaga 1321 tattggataa aaaaacaaga gtatattgct tagtttcatc caccagtcat ccccacagcg 1381 tttggaaggc cataaaaagt gtctaaaatc aatgatcatt gaaagagcac aagagagact 1441 cttacgctgt aatgccactg gggacaaaag tgacagtctc ttaatgggct cttctggagg 1501 ggctcctgaa cattaaaaat tatcagcgaa attaccgaaa gagcttcaag caactggcat 1561 gcttgatcct ctgcgtcggg gcggtgaata ggtgcttcag atgccctctt acccacgggc 1621 tggattcagc tgccccgcta ccagcggaga ccccctaatg agcctctgca attaagttta 1681 ttcatgttaa gtgtgaacgg ggtgcgtgcg gaactgtggg cagctaacag acctgggttc 1741 tttgtgccac aagtgctgcc tttattcggc tcacaaagca gaaaacaaca cccgcaccta 1801 taatggcgcc ctcggctggg tctaagaaac gtggcgagtt gacagagcag agtgggcggg 1861 gttaagacag actgacagcg ggacccatct ccatcctctt attaacgctt aacgagtgcc 1921 ttcctcatgc aatattcatc gccactaata tcatccaagc tctgagctga gctggccact 1981 tatgtaaggc aattatgtaa aatatcagac agggcccaca ctcagaatct gactggggta 2041 gagacgcggg acgagaaccg agagcaagaa ctgaaagtga aagtgaccac taaagggagg 2101 agaggacaga ggggcaggat gtgtcaagat taccagagaa cacttggcca gaaatgcgca 2161 accattggag ctctccggat tacccaaagg ttaacgagtt tgaacgcctc tgcccactcg 2221 cccatctctg atggtttccc aagaactcct caagcaaaat atatataatt gtgtgtatta 2281 tgcacagaca cgagaaaatg ctgtttttct gatctgcatt acagcacatt tgcccgccaa 2341 cgacaatacc acccactcgg tacctcgctg actcctgatg cctgatacct gcgcggtgac 2401 tgtctacaat ctgcataatc aagagaagtt gtgttgaaga cgagcgccac acaaccgttt 2461 ccacaaggtc acccaaggcc ggtgcagatg taggtgaggt ctccataaac agactgaaat 2521 aaacacatcc tccgctggga acaacaaccc cctcacgcct catgcatttc cataagccta 2581 catgcatctc ttccaactta tggagactcg cacctaccaa catccgcaca acaaagatat 2641 acagagcgcg ctccctcagg tcaaggccct gtgggggtct gtgcagaaat aggtcatttg 2701 tcacacatca agtcctgggg caggagatgc attatagatg agaccaaaca gcctgtctcg 2761 gtgagctcta cccactccct gagactagaa atgggggaag ggagcttgag ataacaaccg 2821 ctgcaatcac tgtgtcgatg tttaatatca gcaccaaccg ggaacaataa ggagatgggt 2881 gcattcatgt tcacatctta ccagtcaagt atcatcgaac cggcttgata accacacctc 2941 gtgtaatagc tgagcagata gttgtcattt taaagcgttg gcctttgtcg attatgtaat 3001 gcgcacattc aacacatggt aatatagaaa cggttatgtc gaggttgttt tgtccagaga 3061 tgaccttcac acagttacag ccgctctgca tccacacaaa tggaggactt aatcgtggac 3121 tgcattctta gaaatgatct acaaagacaa ataatgtgaa atcaagaaag gacaaaattt 3181 aagtaagggg atgagggaga gagagaacga ggggcaagga gaaagcatgg ctcctgtctt 3241 tttctgcacc catctgttcg gagtgcaggt ggagctctat tcactcagct ctgcatgtgt 3301 gtttgggggg ggcaggaaga aagggagggc aaaaggaaga gtggagagat ggtgggggct 3361 ggagggatgg ggggttctcg gtgatctctc ctgaagggga taatgggaga gcagcgcttt 3421 gcaatggctg ccatgtagta ccctccctgc acaattagcc aatcagcagc aagctctgcc 3481 agccagaagg acacataaaa gaagaacatt gcagcagagg cacagaagga gcctgcgagg 3541 agctgggaaa tacacacaca acagcagaac cacaacaccc tcccctggac acaccctact 3601 ggggatcact gcttttcttt ttttctgaac catcgcccac gccacacgga gagaaatctc 3661 tctctcatca tcatcctgaa gaaaaccccc ttatcctcat tttcacactg ctgaggaaaa 3721 cctacaatcg cacgggctga gatttcctgg cgaagactgt cctttttcct ttttcttttt 3781 ttttcttttc ctttggaaac tgacatttgc atttctccat tccaagccac ggcgtaataa 3841 tatctgcaag ccagcctgaa gacctgcaaa tcgaaggacc tagatcacta tcatctttgt 3901 acgtcaagaa atcgaattct gcagtcgacg gtaccgcggg cccgggatcc actagttcta 3961 gagcggccgc caccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattcaa 4021 gtttgtacaa aaaagttgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt 4081 agattttgca taaaaaacag actacataat actgtaaaac acaacatatg cagtcactat 4141 gaaccaacta cttagatggt attagtgacc tgtagaattc gagctctaga gctgcagggc 4201 ggccgcgata tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc 4261 tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga 4321 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa 4381 cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa 4441 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc 4501 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat 4561 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt 4621 atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc 4681 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc 4741 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt 4801 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat 4861 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca 4921 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac 4981 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag 5041 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt 5101 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc 5161 gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg 5221 acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgtcgttc 5281 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 5341 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 5401 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 5461 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 5521 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 5581 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 5641 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 5701 ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 5761 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 5821 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 5881 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 5941 ctggcctttt gctggccttt tgctcacatg tt //