LOCUS       Exported                 770 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  Expression of Plum fluorescent protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    gWizPlum
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 770)
  TITLE     Loree Heller plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(78..758)
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     complement(140..163)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(338..357)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     regulatory      755..764
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
ORIGIN
        1 acacacagat ctctagagct ctgatctttt attagccaga agtgatctgg atccaggcgc
       61 ctggtctaga gcggccgcta ggcgccggtg gagtggcggc cctcggcgcg ctcgtactgt
      121 tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca gcttgatgtc ggtcttgtag
      181 gcgccgggca gctgcacggg cttcttggcc atgtaggtgg tcttgacctc ggcgtcgtag
      241 tggccgccgt ccttcagcct cagcctcatc ttcatctcgc ccttcagggc gccgtcctcg
      301 gggtacatcc gctcggagga ggcctcccag cccatggtct tcttctgcat tacggggccg
      361 tcggagggga agttggtgcc gcgcaccttc accttgtaga tgaactcgcc gtcctgcagg
      421 gaggagtcct gggtcacggt caccacgccg ccgtcctcga agttcatcac gcgctcccac
      481 ttgaagccct cggggaagga cagcttcaag tagtcgggga tgtcggcggg gtgcttcacg
      541 taggccttgg agccgtacat gatctgaggg gacaggatgt cccaggcgaa gggcaggggg
      601 ccacccttgg tcaccttcag cctggcggtc tgggtgccct cgtaggggcg gccctcgccc
      661 tcgccctcga tctcgaactc gtggccgttc acggagccct ccatgtgctc cttgaagcgc
      721 atgaactcct tgatgacctc ctcgcccttg ctcaccatgg tggcgaccgg
//
