LOCUS       Exported                 516 bp ds-DNA     linear   SYN 25-MAY-2021
DEFINITION  AAV expression of eNpHR 3.0 fused to EYFP driven by humanized 
            Synapsin promoter for optogenetic inhibition.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-hSyn-eNpHR 3.0-EYFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 516)
  TITLE     eNpHR3.0-EYFP
REFERENCE   2  (bases 1 to 516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 25, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(76..327)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(118..139)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     467..486
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattctc attacacctc
       61 gttctcgtag cagaacttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac
      121 gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg
      181 gtagctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg
      241 ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt
      301 caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc tgttgtagtt
      361 gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc
      421 gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc
      481 gtcgtccttg aagaagatgg tgcgctcctg gacgta
//
