LOCUS       Exported                3424 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  mCherry (red) fluorophore with multiple cloning site for use with 
            inducible N-terminal fusions..
ACCESSION   .
VERSION     .
KEYWORDS    p3E-mCherry-NS-MCS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan 
            A, Chow S, Gwee SS, Stoddart JJ, Nicholson G, Chung R, Cole NJ
  TITLE     A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous 
            System and Neurodegenerative Disease.
  JOURNAL   Zebrafish. 2016 Sep 15.
  PUBMED    27631880
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      268..295
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      387..473
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(626..750)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      752..761
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     primer_bind     1476..1492
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1477..1493
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1533..1628)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1665..1684)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1666..1684)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1689..1705)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1689..1705)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1807..1826)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1818..2627
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1885..1904)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2774..3362
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3263..3282
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct cctacaggtc
      601 actaatacca tctaagtagt tggttcatag tgactgcata tgttgtgttt tacagtatta
      661 tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat cattttacgt
      721 ttctcgttca actttcttgt acaaagtggc tgccaccatg gtgtccaagg gcgaagagga
      781 caacatggcc atcatcaaag agttcatgcg gttcaaggtg cacatggaag gcagcgtgaa
      841 cggccacgag ttcgagattg agggcgaggg cgaaggcaga ccctacgagg gaacacagac
      901 cgccaagctg aaagtgacca agggcggacc cctgcccttc gcctgggata ttctgagccc
      961 ccagtttatg tacggcagca aggcctacgt gaagcacccc gccgacatcc ccgactacct
     1021 gaagctgagc ttccccgagg gcttcaagtg ggagagagtg atgaacttcg aggacggcgg
     1081 cgtcgtgacc gtgacccagg atagctctct gcaggacggc gagttcatct acaaagtgaa
     1141 gctgcggggc accaacttcc ccagcgacgg acccgtgatg cagaaaaaga ccatgggctg
     1201 ggaggccagc agcgagagaa tgtaccctga ggatggcgcc ctgaagggcg agatcaagca
     1261 gcggctgaaa ctgaaggatg gcggccacta cgacgccgaa gtgaaaacca cctacaaggc
     1321 caagaaaccc gtgcagctgc ctggcgccta caacgtgaac atcaagctgg acatcaccag
     1381 ccacaacgag gactacacca tcgtggaaca gtacgagcgg gccgagggca gacactctac
     1441 aggcggaatg gacgagctgt acaagcacgg atccctcgag gtcgacggta tcgataagct
     1501 tgatatcgaa ttccccgggt ctagaactag tgcaacttta ttatacaaag ttggcattat
     1561 aaaaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
     1621 cattatttgg agctccatgg tagcgttaac gcggccgcga tatcccctat agtgagtcgt
     1681 attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt
     1741 acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca
     1801 gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt
     1861 ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag
     1921 gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg
     1981 gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg
     2041 aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac
     2101 tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag
     2161 gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt
     2221 gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga
     2281 ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac
     2341 aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg
     2401 gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg
     2461 ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg
     2521 gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg
     2581 atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta
     2641 attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac
     2701 caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga
     2761 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2821 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2881 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     2941 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     3001 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     3061 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     3121 tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca
     3181 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     3241 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     3301 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     3361 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     3421 tgtt
//
