LOCUS Exported 12442 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION To express the inhibitory scaffold gephyrin tagged to teal fluorescent protein. Expression is Cre dependant. Plasmid can also be used to make lentivirus.. ACCESSION . VERSION . KEYWORDS FU- dio Teal-gephyrin - W SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12442) AUTHORS Villa KL, Berry KP, Subramanian J, Cha JW, Oh WC, Kwon HB, Kubota Y, So PT, Nedivi E TITLE Inhibitory Synapses Are Repeatedly Assembled and Removed at Persistent Sites In Vivo. JOURNAL Neuron. 2016 Feb 3. pii: S0896-6273(16)00011-8. doi: 10.1016/j.neuron.2016.01.010. PUBMED 26853302 REFERENCE 2 (bases 1 to 12442) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12442 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(47..66) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 238..617 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 619..817 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 772..792 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 835..1015 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1062..1187 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1680..1913 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2098..2142 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2440..2557 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2611..3821 /label=UbC promoter /note="human ubiquitin C promoter" primer_bind 3658..3677 /label=hUBCpro-F /note="Human Ubiquitin C (UbC) promoter, forward primer" protein_bind 3874..3907 /label=lox2272 /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with each other, but incompatible with loxP or loxN sites (Lee and Saito, 1988)." protein_bind 3958..3991 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." CDS complement(6271..6978) /codon_start=1 /product="monomeric teal (cyan) fluorescent protein (Ai et al., 2006)" /label=mTFP1 /note="mammalian codon-optimized" /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA VARNSTDGMDELYK" regulatory 6975..6984 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" protein_bind complement(6992..7025) /label=lox2272 /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with each other, but incompatible with loxP or loxN sites (Lee and Saito, 1988)." protein_bind complement(7076..7109) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." misc_feature 7134..7722 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(7187..7207) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7605..7616) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(7725..7741) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7726..7742) /label=pBluescriptKS /note="For pBluescript vector" LTR 8247..8427 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(8453..8470) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 8459..8683 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 8729..9157 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(8816..8835) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 9026..9047 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(9166..9186) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 9171..9500 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 9351..9486 /label=SV40 ori /note="SV40 origin of replication" primer_bind 9413..9432 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 9548..9595 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 9614..9988 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 10118..10239 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(10155..10174) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 10209..10228 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(10288..10304) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(10288..10304) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(10301..10323) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 10312..10328 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(10336..10366) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10381..10402 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(10519..10536) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(10690..11278) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(10770..10789) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(11449..12309) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 12072..12091 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(12310..12414) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta agggtgcagc ggcctccgcg ccgggttttg gcgcctcccg 2641 cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc aggagcgttc 2701 ctgatccttc cgcccggacg ctcaggacag cggcccgctg ctcataagac tcggccttag 2761 aaccccagta tcagcagaag gacattttag gacgggactt gggtgactct agggcactgg 2821 ttttctttcc agagagcgga acaggcgagg aaaagtagtc ccttctcggc gattctgcgg 2881 agggatctcc gtggggcggt gaacgccgat gattatataa ggacgcgccg ggtgtggcac 2941 agctagttcc gtcgcagccg ggatttgggt cgcggttctt gtttgtggat cgctgtgatc 3001 gtcacttggt gagttgcggg ctgctgggct ggccggggct ttcgtggccg ccgggccgct 3061 cggtgggacg gaagcgtgtg gagagaccgc caagggctgt agtctgggtc cgcgagcaag 3121 gttgccctga actgggggtt ggggggagcg cacaaaatgg cggctgttcc cgagtcttga 3181 atggaagacg cttgtaaggc gggctgtgag gtcgttgaaa caaggtgggg ggcatggtgg 3241 gcggcaagaa cccaaggtct tgaggccttc gctaatgcgg gaaagctctt attcgggtga 3301 gatgggctgg ggcaccatct ggggaccctg acgtgaagtt tgtcactgac tggagaactc 3361 gggtttgtcg tctggttgcg ggggcggcag ttatgcggtg ccgttgggca gtgcacccgt 3421 acctttggga gcgcgcgcct cgtcgtgtcg tgacgtcacc cgttctgttg gcttataatg 3481 cagggtgggg ccacctgccg gtaggtgtgc ggtaggcttt tctccgtcgc aggacgcagg 3541 gttcgggcct agggtaggct ctcctgaatc gacaggcgcc ggacctctgg tgaggggagg 3601 gataagtgag gcgtcagttt ctttggtcgg ttttatgtac ctatcttctt aagtagctga 3661 agctccggtt ttgaactatg cgctcggggt tggcgagtgt gttttgtgaa gttttttagg 3721 caccttttga aatgtaatca tttgggtcaa tatgtaattt tcagtgttag actagtaaat 3781 tgtccgctaa attctggccg tttttggctt ttttgttaga cgaagcttgg gctgcaggtc 3841 gactctagag gatcctctag agtcgactcc ggaataactt cgtataggat actttatacg 3901 aagttatgca gaatggtagc tggattgtag ctgctattag caatatgaaa cctcttaata 3961 acttcgtata gcatacatta tacgaagtta tggcgcgccc gtacgtcata gccgtccgat 4021 gaccatgacg tccaccacct cgcctttgtg aagttccaca tactgctctg tctttggagg 4081 tagcatcagc aatccattgg cactgcgcat gctcatcaga cggctgctca tctgattacc 4141 tgtactctgg gcccagggca gtggttcttg gtgatgccaa gtcagtatac accggtggta 4201 ttctgggcga gggtccagct ttacgtcaca tgataaccta gctttgatga tggttggccg 4261 aggatccaag ataccctgca tttttctcag tgcgggtaca acaaagaggt tgcaggtgac 4321 cacagctgat acaggattcc ctggtagtgc aaaaataatt tttcttacac catcaatatc 4381 caaagttgca aatgttgttg gcaagcctgg cttcataaaa accctgccaa aatggatctg 4441 agcatgaaga tcgatgtcca gcacctgctt gagatagtcc ttttccccca tggatacacc 4501 tcctgacgtg atgatgacat cagcacggct gataccctca ttcaaggcat tgagtaagtc 4561 atctgggttg tctcctacaa ttcccaagtt gattgttggg taaccatgtt cctgaattgt 4621 tgctagaagt gttgatcgat tgctgtcccg aatctttcct ggtaagaggt catcttcagg 4681 atttagtagc tcattccctg ttgacataac ggcaaccact ggaaacttat taacttcaac 4741 ttctgtgaca cctacagttg caaggagacc aatttctgaa gggcccatat gggttccttt 4801 tgccaaaaca cactcccctc ttttaatgtc gtggccgatg ggtctgatgt cttggcctgg 4861 ccgagcttgc accagaattc gcacttcaag ttcttcagta ccatcatctg attccctgat 4921 gagttcagta tcttcaactt gcactactgc atcagcaccg caggggattg gagcacctgt 4981 tgtaacccgc atcacttgtc cgggcattac tgtctgagtt ggctgttcac cagcttggga 5041 ttccccaatg atgaaacgat ctcctggacc atcagcagct cgaacagcat agccatcttt 5101 tactgatgct gggaatgggg gtaggttgtc ttttgcatac acatcttgag caaggactcg 5161 ccccattcca tctcggtaat tgatgatttc tgtaccaagc accggagtca tctccagaac 5221 tgtaatgaag gctttgtcca tagacgtcag aggaaaagga gacatgcgat gtcttctagc 5281 caccttggtg atatctacag cactgtgact ggctcttaga atgttctcct tgctgctgca 5341 cctggactgg acttttggtg ttggacaaga agctgtagag aggcgagatg tagcctgggc 5401 acggggcgac tctgaaggag tagtgctaag ggaggctgtg tctcgtggga gcacctgaac 5461 gccacgagaa atgatggagt ctggaatctt tgcagcaaga gctgctgcag taatatgtga 5521 tgaggaacta tcttctgtcg aagctacacc actgtctttc ttttcttctt cctcctcttc 5581 acactgaact cctttgtctt ctgtctgctt atgtgggcta gttgtaggag gtggagaaag 5641 aggaggtggt ggggaaggta aatcttcaag ttcatcatgc acctccttta cttttacaat 5701 ggcatcacgt aaaaggtcaa tggcatgagg tagagctggc agtatgaact gaaagcattc 5761 ctgagatcct ttcttgctac caggcaggtt aattatcaga gttttccctc ttattccaca 5821 cactggtcta gagagcatgc ccagaggtgt aacattaagt gatcccatca gcattgccag 5881 ggccatccct ggcgcttccc gttctattac ttcttttgtg gcctctggag tgacatctcg 5941 tggtgcaaac cctgttccgc cagttgttaa tatcaaatta agttccttct catcacacca 6001 gtctatgagt gtttccttga tttcttctat ttcatctggt actatcttgt atgctgatat 6061 agtcccaccc aacaaagaag gatcctggac gagatctttt agatttatcc cactgcggtc 6121 ttctgcaaga ttcctgaagc agctatcact cactgtgagg actccgacac ggatttgatg 6181 gtcgtggttt gtgaggatca ttccctcggt cgccatgttt cccctcgtgc cgaattcgaa 6241 gcttgagctc gagatctgag tccggccgga cttgtacagc tcgtccatgc cgtcggtgga 6301 gttgcgggcc acggcgctct cgtaaacggt caccttgttg tagtccttgt cgtggttcag 6361 gatctcgatg cggtggtcca caaagtgata gtcgggcagc ttcaccgcct tcttggccct 6421 gtagatggtc ttgaagtcaa cgcggtggtg gccgccgccc tccagcagca gcttgtgctt 6481 gacgtcgccc ttcagcacgc cgtcgcgcac gtacatcctc tcggtggagg cgtcccagcc 6541 ggtggtcttc ttctgcatca cggggccgtt gggggggaag ttctcgccct tgaggtgtat 6601 ctcgtagatg aaggagtcct cctccatgga gatgtcggac ttcaccttca cgatgccctt 6661 gtcctcgaag gtcatggtgc gctcccaaga gtagccctcg gggaaggact gcttgaagta 6721 gttggggatg tcgtcggggt acttggtgaa ggccctgttg ccgtaggcga acgcggtggt 6781 cagaatgtcg taggagaagg gcaggggggc tccctccttc acctccaggt tgatggtgtt 6841 ggtgccgtcg tagggcttgc cctcgccctc gccctcgatc acgaaggcgt ggccattcac 6901 gttgccctcc atcttcagct tgatcttcat gtcgggcttg attacgccca ttgtggtctc 6961 ctcgcccttg ctcaccatgg tggcggctag cataacttcg tataaagtat cctatacgaa 7021 gttatttgcc ttaacccaga aattatcact gttattcttt agaatggtgc aaagaataac 7081 ttcgtataat gtatgctata cgaagttatg aattcgatat caagcttatc gataatcaac 7141 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta 7201 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt 7261 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg 7321 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg 7381 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca 7441 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca 7501 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg 7561 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag 7621 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc 7681 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc 7741 gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt 7801 gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac 7861 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg 7921 ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct 7981 accacacaca aggctacttc cctgattggc agaactacac accagggcca gggatcagat 8041 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag aaggtagaag 8101 aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat gggatggatg 8161 acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt catcacatgg 8221 cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc tgagcctggg 8281 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc 8341 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct 8401 tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc agcctcgact 8461 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg 8521 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg 8581 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg 8641 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga 8701 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg 8761 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct 8821 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat 8881 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt 8941 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg 9001 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac 9061 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta 9121 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt 9181 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 9241 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 9301 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc 9361 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 9421 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg 9481 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc 9541 agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt 9601 gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt 9661 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga 9721 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca 9781 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc 9841 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga 9901 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt 9961 gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc 10021 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca 10081 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa 10141 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 10201 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac 10261 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 10321 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 10381 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 10441 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 10501 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 10561 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 10621 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 10681 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 10741 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 10801 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 10861 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 10921 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 10981 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 11041 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 11101 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 11161 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 11221 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 11281 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 11341 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 11401 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 11461 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 11521 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 11581 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 11641 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 11701 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 11761 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 11821 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 11881 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 11941 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 12001 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 12061 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 12121 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 12181 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 12241 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 12301 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 12361 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 12421 tccccgaaaa gtgccacctg ac //