LOCUS Exported 11729 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pAd-Track Flag-SIRT1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11729) AUTHORS Rodgers JT, Lerin C, Haas W, Gygi SP, Spiegelman BM, Puigserver P TITLE Nutrient control of glucose homeostasis through a complex of PGC-1alpha and SIRT1. JOURNAL Nature 2005 Mar 3;434(7029):113-8. PUBMED 15744310 REFERENCE 2 (bases 1 to 11729) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11729 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 79..181 /label=ITR /note="inverted terminal repeat of human adenovirus serotype 5" enhancer 3440..3743 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 3744..3947 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 4013..4032 /label=T7 /note="T7 promoter, forward primer" promoter 4013..4031 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 4032..4056 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 4071..4093 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 4103..4126 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" CDS 4148..4180 /codon_start=1 /product="leader peptide from bacteriophage T7 gene 10" /label=T7 tag (gene 10 leader) /note="promotes efficient translation in E. coli" /translation="MASMTGGQQMG" CDS 4226..6439 /codon_start=1 /product="mouse NAD+-dependent protein deacetylase sirtuin-1" /label=SIRT1 /note="homolog of budding yeast Sir2" /translation="MADEVALALQAAGSPSAAAAMEAASQPADEPLRKRPRRDGPGLGR SPGEPSAAVAPAAAGCEAASAAAPAALWREAAGAAASAEREAPATAVAGDGDNGSGLRR EPRAADDFDDDEGEEEDEAAAAAAAAAIGYRDNLLLTDGLLTNGFHSCESDDDDRTSHA SSSDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDMTLWQIVINILSE PPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAVDFPD LPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNID TLEQVAGIQRILQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPLAIM KPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINR EPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRPQKELVHLS ELPPTPLHISEDSSSPERTVPQDSSVIATLVDQATNNNVNDLEVSESSCVEEKPQEVQT SRNVENINVENPDFKAVGSSTADKNERTSVAETVRKCWPNRLAKEQISKRLEGNQYLFV PPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPLEDESEIEEFYNGLED DTERPECAGGSGFGADGGDQEVVNEAIATRQELTDVNYPSDKS" regulatory 4280..4289 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" polyA_signal 6650..6771 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" enhancer 6857..7160 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 7161..7364 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" regulatory 7403..7412 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 7409..8125 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 7409..8125 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(7454..7475) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(7715..7734) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 8062..8083 /label=EGFP-C /note="EGFP, forward primer" polyA_signal 8264..8385 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" misc_signal 8528..8678 /label=Ad5 Psi /note="packaging signal for adenovirus serotype 5" repeat_region 8766..8868 /label=ITR /note="inverted terminal repeat of human adenovirus serotype 5" rep_origin 8906..9286 /direction=RIGHT /label=M13 ori /note="M13 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(8993..9012) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 9203..9224 /label=F1ori-F /note="F1 origin, forward primer" CDS complement(9334..10128) /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(9445..9464) /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind 10055..10074 /label=Neo-R /note="Neomycin resistance gene, reverse primer" rep_origin 10960..11548 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 11449..11468 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 11702..11719 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatgga 61 tccatgcatg ttaattaaca tcatcaataa tataccttat tttggattga agccaatatg 121 ataatgaggg ggtggagttt gtgacgtggc gcggggcgtg ggaacggggc gggtgacgta 181 ggttttaggg cggagtaact tgtatgtgtt gggaattgta gttttcttaa aatgggaagt 241 gacgtaacgt gggaaaacgg aagtgacgat ttgaggaagt tgtgggtttt ttggctttcg 301 tttctgggcg taggttcgcg tgcggttttc tgggtgtttt ttgtggactt taaccgttac 361 gtcatttttt agtcctatat atactcgctc tgcacttggc ccttttttac actgtgactg 421 attgagctgg tgccgtgtcg agtggtgttt ttttaatagg ttttcttttt tactggtaag 481 gctgactgtt atggctgccg ctgtggaagc gctgtatgtt gttctggagc gggagggtgc 541 tattttgcct aggcaggagg gtttttcagg tgtttatgtg tttttctctc ctattaattt 601 tgttatacct cctatggggg ctgtaatgtt gtctctacgc ctgcgggtat gtattccccc 661 gggctatttc ggtcgctttt tagcactgac cgatgtgaat caacctgatg tgtttaccga 721 gtcttacatt atgactccgg acatgaccga ggagctgtcg gtggtgcttt ttaatcacgg 781 tgaccagttt ttttacggtc acgccggcat ggccgtagtc cgtcttatgc ttataagggt 841 tgtttttcct gttgtaagac aggcttctaa tgtttaaatg tttttttgtt attttatttt 901 gtgtttatgc agaaacccgc agacatgttt gagagaaaaa tggtgtcttt ttctgtggtg 961 gttccggagc ttacctgcct ttatctgcat gagcatgact acgatgtgct ttcttttttg 1021 cgcgaggctt tgcctgattt tttgagcagc accttgcatt ttatatcgcc gcccatgcaa 1081 caagctattg aattcgttta aactccctct caagtctgta tacggggaca cggacagcct 1141 tttcgtcacc gagcgtggac accggctcat ggaaaccaga ggtaagaaac gcatcaaaaa 1201 gcatggggga aacctggttt ttgaccccga acggccagag ctcacctggc tcgtggaatg 1261 cgagaccgtc tgcggggcct gcggcgcgga tgcctactcc ccggaatcgg tatttctcgc 1321 gcccaagctc tacgccctca aaagtctgca ctgcccctcg tgcggcgcct cctccaaggg 1381 caagctgcgc gccaagggcc acgccgcgga ggggctggac tatgacacca tggtcaaatg 1441 ctacctggcc gacgcgcagg gcgaagaccg gcagcgcttc agcaccagca ggaccagcct 1501 caagcgcacc ctggccagcg cgcagcccgg agcgcacccc ttcaccgtga cccagactac 1561 gctgacgagg accctgcgcc cgtggaaaga catgaccctg gcccgtctgg acgagcaccg 1621 actactgccg tacagcgaaa gccgccccaa cccgcgaaac gaggagatat gctggatcga 1681 gatgccgtag agcaggtgac cgagctgtgg gaccgcctgg aactgcttgg tcaaacgctc 1741 aaaagcatgc ctacggcgga cggtctcaaa ccgttgaaaa actttgcttc cttgcaagaa 1801 ctgctatcgc tgggcggcga gcgccttctg gcggatttgg tcagggaaaa catgcgagtc 1861 agggacatgc ttaacgaagt ggcccccctg ctcagggatg acggcagctg cagctctctt 1921 aactaccagt tgcagccggt aataggtgtg atttacgggc ccaccggctg cggtaagtcg 1981 cagctgctca ggaacctgct ttcttcccag ctgatctccc ctaccccgga aaccgttttc 2041 ttcatcgccc cgcaggtaga catgatcccc ccatctgaac tcaaagcgtg ggaaatgcaa 2101 atctgtgagg gtaactacgc ccctgggccg gatggaacca ttataccgca gtctggcacc 2161 ctccgcccgc gctttgtaaa aatggcctat gacgatctca tcctggaaca caactatgac 2221 gttagtgatc ccagaaatat cttcgcccag gccgccgccc gtgggcccat tgccatcatt 2281 atggacgaat gcatggaaaa tcttggaggt cacaagggcg tctccaagtt cttccacgca 2341 tttccttcta agctacatga caaatttccc aagtgcaccg gatacactgt gctggtggtt 2401 ctgcacaaca tgaatccccg gagggatatg gctgggaaca tagccaacct aaaaatacag 2461 tccaagatgc atctcatatc cccacgtatg cacccatccc agcttaaccg ctttgtaaac 2521 acttacacca agggcctgcc cctggcaatc agcttgctac tgaaagacat ttttaggcac 2581 cacgcccagc gctcctgcta cgactggatc atctacaaca ccaccccgca gcatgaagct 2641 ctgcagtggt gctacctcca ccccagagac gggcttatgc ccatgtatct gaacatccag 2701 agtcaccttt accacgtcct ggaaaaaata cacaggaccc tcaacgaccg agaccgctgg 2761 tcccgggcct accgcgcgcg caaaacccct aaataaagac agcaagacac ttgcttgatc 2821 caaatccaaa cagagtctgg ttttttattt atgttttaaa ccgcattggg aggggaggaa 2881 gccttcaggg cagaaacctg ctggcgcaga tccaacagct gctgagaaac gacattaagt 2941 tcccgggtca aagaatccaa ttgtgccaaa agagccgtca acttgtcatc gcgggcggat 3001 gaacgggaag ctgcactgct tgcaagcggg ctcaggaaag caaagtcagt cacaatcccg 3061 cgggcggtgg ctgcagcggc tgaagcggcg gcggaggctg cagtctccaa cggcgttcca 3121 gacacggtct cgtaggtcaa ggtagtagag tttgcgggca ggacggggcg accatcaatg 3181 ctggagccca tcacattctg acgcaccccg gcccatgggg gcatgcgcgt tgtcaaatat 3241 gagctcacaa tgcttccatc aaacgagttg gtgctcatgg cggcggcggc tgctgcaaaa 3301 cagatacaaa actacataag acccccacct tatatattct ttcccaccct taaccacgcc 3361 cagatcctct agcagtgata aacgtctaat agtaatcaat tacggggtca ttagttcata 3421 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 3481 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 3541 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac 3601 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 3661 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 3721 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat 3781 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt 3841 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc 3901 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc 3961 gtcagatccg ctagagatct ggtaccgagc tcggatctcg atcccgcgaa attaatacga 4021 ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac 4081 tttaagaagg agatatacca tggactacaa agacgatgac gataaagcaa gaagagcatc 4141 tgtgcatatg gctagcatga ctggtggaca gcaaatgggt cgcggatccg aattcgagct 4201 ccgtcgacag agaggcagtt ggaagatggc ggacgaggtg gcgctcgccc ttcaggccgc 4261 cggctcccct tccgcggcgg ccgccatgga ggccgcgtcg cagccggcgg acgagccgct 4321 ccgcaagagg ccccgccgag acgggcctgg cctcgggcgc agcccgggcg agccgagcgc 4381 agcagtggcg ccggcggccg cggggtgtga ggcggcgagc gccgcggccc cggcggcgct 4441 gtggcgggag gcggcagggg cggcggcgag cgcggagcgg gaggccccgg cgacggccgt 4501 ggccggggac ggagacaatg ggtccggcct gcggcgggag ccgagggcgg ccgacgactt 4561 cgacgacgac gagggcgagg aggaggacga ggcggcggcg gcagcggcgg cggcagcgat 4621 cggctaccga gacaacctcc tgttgaccga tggactcctc actaatggct ttcattcctg 4681 tgaaagtgat gacgatgaca gaacgtcaca cgccagctct agtgactgga ctccgcggcc 4741 gcggataggt ccatatactt ttgttcagca acatctcatg attggcaccg atcctcgaac 4801 aattcttaaa gatttattac cagaaacaat tcctccacct gagctggatg atatgacgct 4861 gtggcagatt gttattaata tcctttcaga accaccaaag cggaaaaaaa gaaaagatat 4921 caatacaatt gaagatgctg tgaagttact gcaggagtgt aaaaagataa tagttctgac 4981 tggagctggg gtttctgtct cctgtgggat tcctgacttc agatcaagag acggtatcta 5041 tgctcgcctt gcggtggact tcccagacct cccagaccct caagccatgt ttgatattga 5101 gtattttaga aaagacccaa gaccattctt caagtttgca aaggaaatat atcccggaca 5161 gttccagccg tctctgtgtc acaaattcat agctttgtca gataaggaag gaaaactact 5221 tcgaaattat actcaaaata tagatacctt ggagcaggtt gcaggaatcc aaaggatcct 5281 tcagtgtcat ggttcctttg caacagcatc ttgcctgatt tgtaagtaca aagttgattg 5341 tgaagctgtt cgtggagaca tttttaatca ggtagttcct cggtgcccta ggtgcccagc 5401 tgatgagcca cttgccatca tgaagccaga gattgtcttc tttggtgaaa acttaccaga 5461 acagtttcat agagccatga agtatgacaa agatgaagtt gacctcctca ttgttattgg 5521 atcttctctg aaagtgagac cagtagcact aattccaagt tctatacccc atgaagtgcc 5581 tcaaatatta ataaataggg aacctttgcc tcatctacat tttgatgtag agctccttgg 5641 agactgcgat gttataatta atgagttgtg tcataggcta ggtggtgaat atgccaaact 5701 ttgttgtaac cctgtaaagc tttcagaaat tactgaaaaa cctccacgcc cacaaaagga 5761 attggttcat ttatcagagt tgccaccaac acctcttcat atttcggaag actcaagttc 5821 acctgaaaga actgtaccac aagactcttc tgtgattgct acacttgtag accaagcaac 5881 aaacaacaat gttaatgatt tagaagtatc tgaatcaagt tgtgtggaag aaaaaccaca 5941 agaagtacag actagtagga atgttgagaa cattaatgtg gaaaatccag attttaaggc 6001 tgttggttcc agtactgcag acaaaaatga aagaacttca gttgcagaaa cagtgagaaa 6061 atgctggcct aatagacttg caaaggagca gattagtaag cggcttgagg gtaatcaata 6121 cctgtttgta ccaccaaatc gttacatatt ccacggtgct gaggtatact cagactctga 6181 agatgacgtc ttgtcctcta gttcctgtgg cagtaacagt gacagtggca catgccagag 6241 tccaagttta gaagaaccct tggaagatga aagtgaaatt gaagaattct acaatggctt 6301 ggaagatgat acggagaggc ccgaatgtgc tggaggatct ggatttggag ctgatggagg 6361 ggatcaagag gttgttaatg aagctatagc tacaagacag gaattgacag atgtaaacta 6421 tccatcagac aaatcataac actattgaag ctgtccggat tcaggaattg gtcgacaagc 6481 ttgcggccgc actcgagcct aagcttctag ataagatatc cgatccaccg gatctagata 6541 actgatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc 6601 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat 6661 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 6721 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aacgcggagg 6781 tttatcgacg atctgctagt gattaatagt aatcaattac ggggtcatta gttcatagcc 6841 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 6901 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 6961 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 7021 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 7081 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat 7141 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc 7201 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt 7261 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa 7321 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc 7381 agatccgcta gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg 7441 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc 7501 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac 7561 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg 7621 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga 7681 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc 7741 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt 7801 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt 7861 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa 7921 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga 7981 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga 8041 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac 8101 tctcggcatg gacgagctgt acaagtccgg actcagatcc accggatcta gataactgat 8161 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct 8221 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc 8281 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 8341 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaacgcg atcaagctag 8401 cttgctagac tcgactgact ataataataa aacgccaact ttgacccgga acgcggaaaa 8461 cacctgagaa aaacacctgg gcgagtctcc acgtaaacgg tcaaagtccc cgcggcccta 8521 gacaaatatt acgcgctatg agtaacacaa aattattcag atttcacttc ctcttattca 8581 gttttcccgc gaaaatggcc aaatcttact cggttacgcc caaatttact acaacatccg 8641 cctaaaaccg cgcgaaaatt gtcacttcct gtgtacaccg gcgcacacca aaaacgtcac 8701 ttttgccaca tccgtcgctt acatgtgttc cgccacactt gcaacatcac acttccgcca 8761 cactactacg tcacccgccc cgttcccacg ccccgcgcca cgtcacaaac tccaccccct 8821 cattatcata ttggcttcaa tccaaaataa ggtatattat tgatgatgtt aattaagaat 8881 taattcgatc ctgaatggcg aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt 8941 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc 9001 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg 9061 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat 9121 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg 9181 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct 9241 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa 9301 aatgagctga tttaacaaaa attttaacaa aattcagaag aactcgtcaa gaaggcgata 9361 gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc 9421 ccattcgccg ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg 9481 gtccgccaca cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat 9541 gatattcggc aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgct 9601 cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc 9661 atcctgatcg acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc 9721 ttggtggtcg aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc 9781 catgatggat actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac 9841 ttcgcccaat agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca 9901 aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc tcgtcttgca gttcattcag 9961 ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa 10021 cacggcggca tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc 10081 cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc 10141 tcatcctgtc tcttgatcag agcttgatcc cctgcgccat cagatccttg gcggcaagaa 10201 agccatccag tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa 10261 ttccggttcg cttgctgtcc ataaaaccgc ccagtctagc tatcgccatg taagcccact 10321 gcaagctacc tgctttctct ttgcgcttgc gttttccctt gtccagatag cccagtagct 10381 gacattcatc cggggtcagc accgtttctg cggactggct ttctacgtga aaaggatcta 10441 ggtgaagatc ctttttgata atctcatggc tgcagcaatg gcaacaacgt tgcgcaaact 10501 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 10561 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 10621 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 10681 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 10741 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 10801 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 10861 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 10921 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 10981 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 11041 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 11101 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 11161 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 11221 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 11281 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 11341 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 11401 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 11461 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 11521 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 11581 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 11641 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 11701 cagcgagtca gtgagcgagg aagcggaag //