LOCUS Exported 867 bp ds-DNA linear SYN 20-MAY-2021 DEFINITION Mammalian expression of human NSF full-length (744 aa). ACCESSION . VERSION . KEYWORDS pcDNA3-Flag-NSF SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 867) AUTHORS Belluzzi E, Gonnelli A, Cirnaru MD, Marte A, Plotegher N, Russo I, Civiero L, Cogo S, Carrion MP, Franchin C, Arrigoni G, Beltramini M, Bubacco L, Onofri F, Piccoli G, Greggio E TITLE LRRK2 phosphorylates pre-synaptic N-ethylmaleimide sensitive fusion (NSF) protein enhancing its ATPase activity and SNARE complex disassembling rate. JOURNAL Mol Neurodegener. 2016 Jan 13;11(1):1. doi: 10.1186/s13024-015-0066-z. PUBMED 26758690 REFERENCE 2 (bases 1 to 867) AUTHORS . TITLE Direct Submission JOURNAL Exported May 20, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..867 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 52..71 /label=T7 /note="T7 promoter, forward primer" promoter 52..70 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 136..145 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 145..168 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" CDS 184..207 /codon_start=1 /product="peptide that binds Strep-Tactin(R), an engineered form of streptavidin" /label=Strep-Tag II /translation="WSHPQFEK" CDS 244..267 /codon_start=1 /product="peptide that binds Strep-Tactin(R), an engineered form of streptavidin" /label=Strep-Tag II /translation="WSHPQFEK" protein_bind 280..304 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" ORIGIN 1 cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac 61 tcactatagg gagacccaag cttggtaccg agctcggatc cactagtaac ggccgccagt 121 gtgctggaat tcgccgccac catggattat aaagatgatg atgataaagg gtcggccgcc 181 agctggagcc accctcagtt cgagaaggga ggaggaagcg gcggaggcag cggaggagga 241 agctggagcc acccgcagtt cgagaaagga gctagatcaa caagtttgta caaaaaagca 301 ggcttcgcgg gccggagcat gcaagcggca agatgtccta cagatgaatt atctttaacc 361 aattgtgcag ttgtgaatga aaaggatttc cagtctggcc agcatgtgat tgtgaggacc 421 tctcccaatc acaggtacac atttacactg aagacacatc catcggtggt tccagggagc 481 attgcattca gtttacctca gagaaaatgg gctgggcttt ctattgggca agaaatagaa 541 gtctccttat atacatttga caaagccaaa cagtgtattg gcacaatgac catcgagatt 601 gatttcctgc agaaaaaaag cattgactcc aacccttatg acaccgacaa gatggcagca 661 gaatttattc agcaattcaa caaccaggcc ttctcagtgg gacaacagct tgtctttagc 721 ttcaatgaaa agctttttgg cttactggtg aaggacattg aagccatgga tcctagcatc 781 ctgaagggag agcctgcgac agggaaaagg cagaagattg aagtaggact ggttgttgga 841 aacagtcaag ttgcatttga aaaagca //