LOCUS Exported 4722 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Localization: C1 Cloning Vector, Excitation: 462, Emission: 492. ACCESSION . VERSION . KEYWORDS mTFP1-C1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4722) AUTHORS Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE TITLE Hue-shifted monomeric variants of Clavularia cyan fluorescent protein: identification of the molecular determinants of color and applications in fluorescence imaging. JOURNAL BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13. PUBMED 18325109 REFERENCE 2 (bases 1 to 4722) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4722 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 68..371 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 372..575 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 526..546 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 614..623 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 620..1327 /codon_start=1 /product="monomeric teal (cyan) fluorescent protein (Ai et al., 2006)" /label=mTFP1 /note="mammalian codon-optimized" /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA VARNSTDGMDELYK" misc_feature 1328..1393 /label=MCS /note="multiple cloning site" polyA_signal 1517..1638 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1554..1573) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1608..1627 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(1645..2100) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1782..1803) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 1994..2013 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2127..2231 /gene="bla" /label=AmpR promoter promoter 2233..2590 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2256..2276) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2441..2576 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2503..2522 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2625..3419 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2679..2698) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3289..3308 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(3607..3626) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 3651..3698 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4027..4615 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4516..4535 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg 61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc 121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt 181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc 241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 601 agcgctaccg gtcgccacca tggtgagcaa gggcgaggag accacaatgg gcgtaatcaa 661 gcccgacatg aagatcaagc tgaagatgga gggcaacgtg aatggccacg ccttcgtgat 721 cgagggcgag ggcgagggca agccctacga cggcaccaac accatcaacc tggaggtgaa 781 ggagggagcc cccctgccct tctcctacga cattctgacc accgcgttcg cctacggcaa 841 cagggccttc accaagtacc ccgacgacat ccccaactac ttcaagcagt ccttccccga 901 gggctactct tgggagcgca ccatgacctt cgaggacaag ggcatcgtga aggtgaagtc 961 cgacatctcc atggaggagg actccttcat ctacgagata cacctcaagg gcgagaactt 1021 cccccccaac ggccccgtga tgcagaagaa gaccaccggc tgggacgcct ccaccgagag 1081 gatgtacgtg cgcgacggcg tgctgaaggg cgacgtcaag cacaagctgc tgctggaggg 1141 cggcggccac caccgcgttg acttcaagac catctacagg gccaagaagg cggtgaagct 1201 gcccgactat cactttgtgg accaccgcat cgagatcctg aaccacgaca aggactacaa 1261 caaggtgacc gtttacgaga gcgccgtggc ccgcaactcc accgacggca tggacgagct 1321 gtacaagtcc ggactcagat ctcgagctca agcttcgaat tctgcagtcg acggtaccgc 1381 gggcccggga tccaccggat ctagataact gatcataatc agccatacca catttgtaga 1441 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa 1501 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag 1561 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 1621 actcatcaat gtatcttaac gcgtaaattg taagcgttaa tattttgtta aaattcgcgt 1681 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt 1741 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc 1801 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg 1861 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac 1921 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg 1981 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag 2041 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt 2101 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 2161 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 2221 aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg 2281 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 2341 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 2401 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc 2461 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga 2521 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg 2581 cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg 2641 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac 2701 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg 2761 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc 2821 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg 2881 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc 2941 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc 3001 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta 3061 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg 3121 cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg 3181 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat 3241 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc 3301 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta 3361 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag 3421 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt 3481 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg 3541 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag 3601 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa 3661 aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca 3721 gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc 3781 ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt 3841 cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa 3901 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa 3961 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg 4021 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc 4081 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac 4141 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca 4201 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt 4261 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc 4321 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg 4381 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc 4441 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac 4501 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct 4561 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc 4621 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt 4681 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gc //