LOCUS Exported 6036 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Localization: Gap Junctions, Excitation: 587, Emission: 610. ACCESSION . VERSION . KEYWORDS mCherry-Cx43-7 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6036) TITLE Davidson lab - mCherry constructs REFERENCE 2 (bases 1 to 6036) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6036 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 68..371 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 372..575 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 526..546 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 1992..2001 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1998..2708 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(2143..2161) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 2411..2430 /label=mCherry-F /note="mCherry, forward primer" polyA_signal 2831..2952 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2868..2887) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2922..2941 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(2959..3414) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3096..3117) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 3308..3327 /label=F1ori-R /note="F1 origin, reverse primer" promoter 3441..3545 /gene="bla" /label=AmpR promoter promoter 3547..3904 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(3570..3590) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 3755..3890 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3817..3836 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3939..4733 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3993..4012) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 4603..4622 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(4921..4940) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 4965..5012 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 5341..5929 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5830..5849 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg 61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc 121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt 181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc 241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 601 agcgctaccg gactcagatc tcgagctcaa gcttcgaatt cggcagcact tttctttcat 661 tggggggaag gcgtgaggaa agtaccaaac agcagcagac ttttaaactt taaacagaca 721 ggtctgagag cctgaactct catttttcct ttgacttcag cctccaagga gttccaccaa 781 ctttggcgcg ccggcttcac tttcattaag tgaaagagag gtgcccagac atgggtgact 841 ggagtgcctt ggggaagctt ctggacaagg tccaagccta ctccaccgct ggagggaagg 901 tgtggctgtc agtgctcttc atattcagaa tcctgctcct ggggacagct gttgagtcag 961 cttggggtga tgaacagtct gcctttcgct gtaacactca acaacctggc tgcgaaaacg 1021 tctgctatga caagtccttc cccatctctc acgtgcgctt ctgggtcctt cagatcatat 1081 tcgtgtctgt gcccacactc ctgtacttgg cccatgtgtt ctatgtgatg aggaaggaag 1141 agaagctaaa caagaaagaa gaggagctca aagtggccca gactgacggg gtcaacgtgg 1201 agatgcacct gaagcagatt gaaatcaaga agttcaagta cgggattgaa gagcacggca 1261 aggtgaaaat gaggggcggc ttgctgagaa cctacatcat cagcatcctc ttcaagtctg 1321 tcttcgaggt ggccttcctg ctcatccagt ggtacatcta tgggttcagc ttgagcgcgg 1381 tctacacctg caagagagat ccctgccccc accaggtaga ctgcttcctc tcacgtccca 1441 cggagaaaac catcttcatc atcttcatgc tggtggtgtc cttggtgtct ctcgctttga 1501 acatcattga gctcttctac gtcttcttca agggcgttaa ggatcgcgtg aagggaagaa 1561 gcgatcctta ccacgccacc actggcccac tgagcccatc aaaagactgc ggatctccaa 1621 aatacgccta cttcaatggc tgctcctcac caacggctcc actctcgcct atgtctcctc 1681 ctgggtacaa gctggttact ggtgacagaa acaattcctc gtgccgcaat tacaacaagc 1741 aagctagcga gcaaaactgg gcgaactaca gcgcagagca aaatcgcatg gggcaggccg 1801 gaagcaccat ctccaactcg cacgcccagc cgttcgattt ccccgacgac aaccagaatg 1861 ccaaaaaagt tgctgctgga catgaactcc agccattagc catcgtggac caacgacctt 1921 ccagcagagc cagcagccgc gccagcagca ggcctcggcc tgatgacctg gagattgcgg 1981 atccaccggt cgccaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg 2041 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg 2101 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca 2161 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca 2221 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg 2281 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg 2341 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc 2401 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga 2461 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg 2521 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc 2581 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca 2641 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt 2701 acaagtaaag cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt 2761 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat 2821 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 2881 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 2941 caatgtatct taaggcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt 3001 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat 3061 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat 3121 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac 3181 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc 3241 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga 3301 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca 3361 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg 3421 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 3481 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 3541 agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc 3601 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag 3661 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 3721 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc 3781 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 3841 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg 3901 caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc 3961 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga 4021 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt 4081 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat 4141 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg 4201 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg 4261 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc 4321 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga 4381 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag 4441 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc 4501 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg 4561 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata 4621 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg 4681 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac 4741 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc 4801 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat 4861 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac 4921 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca 4981 gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg 5041 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt 5101 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc 5161 ggcaggccct gccatagcct caggttactc atatatactt tagattgatt taaaacttca 5221 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc 5281 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 5341 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 5401 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 5461 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt 5521 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 5581 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 5641 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 5701 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 5761 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 5821 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 5881 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 5941 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 6001 gttatcccct gattctgtgg ataaccgtat taccgc //