LOCUS Exported 7476 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pEF myc Gal cyclin T1 (1-726) (P#1026) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7476) AUTHORS Taube R, Lin X, Irwin D, Fujinaga K, Peterlin BM TITLE Interaction between P-TEFb and the C-terminal domain of RNA polymerase II activates transcriptional elongation from sites upstream or downstream of target genes. JOURNAL Mol Cell Biol. 2002 Jan . 22(1):321-31. PUBMED 11739744 REFERENCE 2 (bases 1 to 7476) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7476 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(10..28) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 96..200 /gene="bla" /label=AmpR promoter CDS 201..1061 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(419..438) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1232..1820 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1721..1740 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 1974..1991 /label=L4440 /note="L4440 vector, forward primer" protein_bind 2108..2129 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 2144..2174 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2182..2198 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2187..2209 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 2206..2222 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 2206..2222 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" promoter 2336..3514 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2567..3505 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3462..3482 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" CDS 3586..3603 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 3613..3642 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 3658..4098 /codon_start=1 /gene="Saccharomyces cerevisiae GAL4 (truncated)" /product="DNA binding domain of the GAL4 transcriptional activator" /label=GAL4 DNA binding domain /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS" polyA_signal 6377..6771 /label=-beta-globin poly(A) /note="human beta-globin polyadenylation signal" primer_bind complement(7082..7099) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(7082..7098) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7091..7113) /label=M13/pUC Forward /note="In lacZ gene" primer_bind complement(7307..7326) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 7426..7448 /label=pGEX 3' /note="pGEX vectors, reverse primer" ORIGIN 1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc 1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc 2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg 2221 acatgattac gaattaattc gagctcgccc agctttgcaa agatggataa agttttaaac 2281 agagaggaat ctttgcagct aatggacctt ctaggtcttg aaaggagtgg gaattggctc 2341 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg 2401 ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt 2461 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt 2521 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg 2581 tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc 2641 cacctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag 2701 ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg 2761 gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga 2821 taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga 2881 tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg 2941 cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg 3001 gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct 3061 cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc 3121 gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg 3181 gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc 3241 agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt 3301 ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt 3361 tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc 3421 cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt 3481 tcaaagtttt tttcttccat ttcaggtgtc gtgaggaatt agcttgctta catttgcttc 3541 tgacacaact gtgttcacta gcaacctcaa acagacacca tgccgcatca ccaccatcat 3601 cacgccatgc cggagcagaa gctgatatcc gaggaggacc tggccatggc cggatccatg 3661 aagctactgt cttctatcga acaagcatgc gatatttgcc gacttaaaaa gctcaagtgc 3721 tccaaagaaa aaccgaagtg cgccaagtgt ctgaagaaca actgggagtg tcgctactct 3781 cccaaaacca aaaggtctcc gctgactagg gcacatctga cagaagtgga atcaaggcta 3841 gaaagactgg aacagctatt tctactgatt tttcctcgag aagaccttga catgattttg 3901 aaaatggatt ctttacagga tataaaagca ttgttaacag gattatttgt acaagataat 3961 gtgaataaag atgccgtcac agatagattg gcttcagtgg agactgatat gcctctaaca 4021 ttgagacagc atagaataag tgcgacatca tcatcggaag agagtagtaa caaaggtcaa 4081 agacagttga ctgtatcgcc ggaattcccc atggagggag agaggaagaa caacaacaaa 4141 cggtggtatt tcactcgaga acagctggaa aatagcccat cccgtcgttt tggcgtggac 4201 ccagataaag aactttctta tcgccagcag gcggccaatc tgcttcagga catggggcag 4261 cgtcttaacg tctcacaatt gactatcaac actgctatag tatacatgca tcgattctac 4321 atgattcagt ccttcacaca gttccctgga aattctgtgg ctccagcagc cttgtttcta 4381 gcagctaaag tggaggagca gcccaaaaaa ttggaacatg tcatcaaggt agcacatact 4441 tgtctccatc ctcaggaatc ccttcctgat actagaagtg aggcttattt gcaacaagtt 4501 caagatctgg tcattttaga aagcataatt ttgcagactt taggctttga actaacaatt 4561 gatcacccac atactcatgt agtaaagtgc actcaacttg ttcgagcaag caaggactta 4621 gcacagactt cttacttcat ggcaaccaac agcctgcatt tgaccacatt tagcctgcag 4681 tacacacctc ctgtggtggc ctgtgtctgc attcacctgg cttgcaagtg gtccaattgg 4741 gagatcccag tctcaactga cgggaagcac tggtgggagt atgttgacgc cactgtgacc 4801 ttggaacttt tagatgaact gacacatgag tttctacaga ttttggagaa aactcccaac 4861 aggctcaaac gcatttggaa ttggagggca tgcgaggctg ccaagaaaac aaaagcagat 4921 gaccgaggaa cagatgaaaa gacttcagag cagacaatcc tcaatatgat ttcccagagc 4981 tcttcagaca caaccattgc aggtttaatg agcatgtcaa cttctaccac aagtgcagtg 5041 ccttccctgc cagtctccga agagtcatcc agcaacttaa ccagtgtgga gatgttgccg 5101 ggcaagcgtt ggctgtcctc ccaaccttct ttcaaactag aacctactca gggtcatcgg 5161 actagtgaga atttagcact tacaggagtt gatcattcct taccacagga tggttcaaat 5221 gcatttattt cccagaagca gaatagtaag agtgtgccat cagctaaagt gtcactgaaa 5281 gaataccgcg cgaagcatgc agaagaattg gctgcccaga agaggcaact ggagaacatg 5341 gaagccaatg tgaagtcaca atatgcatat gctgcccaga atctcctttc tcatcatgat 5401 agccattctt cagtcattct aaaaatgccc atagagggtt cagaaaaccc cgagcggcct 5461 tttctggaaa aggctgacaa aacagctctc aaaatgagaa tcccagtggc aggtggagat 5521 aaagctgcgt cttcaaaacc agaggagata aaaatgcgca taaaagtcca tgctgcagct 5581 gataagcaca attctgtaga ggacagtgtt acaaagagcc gagagcacaa agaaaagcac 5641 aagactcacc catctaatca tcatcgtcat cataatcacc actcacacaa gcactctcat 5701 tcccaacttc cagttggtac tgggaacaaa cgtcctggtg atccaaaaca tagtagccag 5761 acaagcaact tagcacataa aacctatagc ttgtctagtt cttttccctc ttccagttct 5821 actcgtaaaa ggggaccctc tgaagagact ggaggggctg tgtttgatca tccagccaag 5881 attgccaaga gtactaaatc ctcttcccta aatttctcct tcccttcact tcctacaatg 5941 ggtcagatgc ctgggcatag ctcagacaca agtggccttt ccttttcaca gcccagctgt 6001 aaaactcgtg tccctcattc gaaactggat aaagggccca ctggggccaa tggtcacaac 6061 acgacccaga caatagacta tcaagacact gtgaatatgc ttcactccct gctcagtgcc 6121 cagggtgttc agcccactca gcctactgca tttgaatttg ttcgtcctta tagtgactat 6181 ctgaatcctc ggtctggtgg aatctcctcg agatctggca atacagacaa accccggcca 6241 ccatctctgc catcagaacc tcctccacca cttccaccct tcctaagtaa tctagaaatt 6301 caccccacca gtgcaggctg cctatcagaa agtggtggct ggtgtggcta atgccctggc 6361 ccacaagtat cactaagctc gctttcttgc tgtccaattt ctattaaagg ttcctttgtt 6421 ccctaagtcc aactactaaa ctgggggata ttatgaaggg ccttgagcat ctggattctg 6481 cctaataaaa aacatttatt ttcattgcaa tgatgtattt aaattatttc tgaatatttt 6541 actaaaaagg gaatgtggga ggtcagtgca tttaaaacat aaagaaatga agagctagtt 6601 caaaccttgg gaaaatacac tatatcttaa actccatgaa agaaggtgag gctgcaaaca 6661 gctaatgcac attggcaaca gcccctgatg cctatgcctt attcatccct cagaaaagga 6721 ttcaagtaga ggcttgattt ggaggttaaa gttttgctat gctgtatttt acattactta 6781 ttgttttagc tgtcctcatg aatgtctttt cactacccat ttgcttatcc tgcatctctc 6841 agccttgact ccactcagtt ctcttgctta gagataccac ctttcccctg aagtgttcct 6901 tccatgtttt acggcgagat ggtttctcct cgcctggcca ctcagcctta gttgtctctg 6961 ttgtcttata gaggtctact tgaagaagga aaaacagggg gcatggtttg actgtcctgt 7021 gagcccttct tccctgcctc ccccactcac agtgacccgg aatctgcagc ccaagcttgg 7081 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc 7141 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc 7201 gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc 7261 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg 7321 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg 7381 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt 7441 gtcagaggtt ttcaccgtca tcaccgaaac gcgcga //