LOCUS       Exported                 849 bp ds-DNA     linear   SYN 29-MAY-2021
DEFINITION  Expresses kinase-inactive mutant of Akt  with HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    pCDNA3-HA-Akt1-K179M
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Zeng M, van der Donk WA, Chen J
  TITLE     Lanthionine synthetase C-like protein 2 (LanCL2) is a novel 
            regulator of Akt.
  JOURNAL   Mol Biol Cell. 2014 Dec 1;25(24):3954-61. doi: 
            10.1091/mbc.E14-01-0004. Epub 2014 Oct 1.
  PUBMED    25273559
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 29, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             132..158
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ctctctggct actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagcttggt accgagctcg gatccactag taacggccgc cagtgtgctg
      121 gaattgaaat gtacccatac gacgtcccag actacgctgg gaattcaagc gacgtggcta
      181 ttgtgaagga gggttggctg cacaaacgag gggagtacat caagacctgg cggccacgct
      241 acttcctcct caagaatgat ggcaccttca ttggctacaa ggagcggccg caggatgtgg
      301 accaacgtga ggctcccctc aacaacttct ctgtggcgca gtgccagctg atgaagacgg
      361 agcggccccg gcccaacacc ttcatcatcc gctgcctgca gtggaccact gtcatcgaac
      421 gcaccttcca tgtggagact cctgaggagc gggaggagtg gacaaccgcc atccagactg
      481 tggctgacgg cctcaagaag caggaggagg aggagatgga cttccggtcg ggctcaccca
      541 gtgacaactc aggggctgaa gagatggagg tgtccctggc caagcccaag caccgcgtga
      601 ccatgaacga gtttgagtac ctgaagctgc tgggcaaggg cactttcggc aaggtgatcc
      661 tggtgaagga gaaggccaca ggccgctact acgccatgat gatcctcaag aaggaagtca
      721 tcgtagccaa ggacgaagtg gcccacacac tcaccgagaa ccgcgtcctg cagaactcca
      781 ggcacccctt cctcacagcc ctgaagtact ctttccagac ccacgaccgc ctctgctttg
      841 tcatggagt
//
