LOCUS Exported 831 bp ds-DNA linear SYN 09-JUL-2021 DEFINITION Expresses HA tagged aa1-149 of AKT1 . ACCESSION . VERSION . KEYWORDS pCDNA3-HA-Akt1-aa1-149 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 831) AUTHORS Zeng M, van der Donk WA, Chen J TITLE Lanthionine synthetase C-like protein 2 (LanCL2) is a novel regulator of Akt. JOURNAL Mol Biol Cell. 2014 Dec 1;25(24):3954-61. doi: 10.1091/mbc.E14-01-0004. Epub 2014 Oct 1. PUBMED 25273559 REFERENCE 2 (bases 1 to 831) AUTHORS . TITLE Direct Submission JOURNAL Exported Jul 9, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..831 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 46..65 /label=T7 /note="T7 promoter, forward primer" promoter 46..64 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 132..158 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" promoter complement(642..660) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(643..660) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(680..697) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 686..797 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" ORIGIN 1 ctctctggct actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta 61 tagggagacc caagcttggt accgagctcg gatccactag taacggccgc cagtgtgctg 121 gaattgaaat gtacccatac gacgtcccag actacgctgg gaattcaagc gacgtggcta 181 ttgtgaagga gggttggctg cacaaacgag gggagtacat caagacctgg cggccacgct 241 acttcctcct caagaatgat ggcaccttca ttggctacaa ggagcggccg caggatgtgg 301 accaacgtga ggctcccctc aacaacttct ctgtggcgca gtgccagctg atgaagacgg 361 agcggccccg gcccaacacc ttcatcatcc gctgcctgca gtggaccact gtcatcgaac 421 gcaccttcca tgtggagact cctgaggagc gggaggagtg gacaaccgcc atccagactg 481 tggctgacgg cctcaagaag caggaggagg aggagatgga cttccggtcg ggctcaccca 541 gtgacaactc aggggctgaa gagatggagg tgtccctggc caagcccaag caccgcgtga 601 ccatgaacga gtagctcgag catgcatcta gagggcccta ttctatagtg tcacctaaat 661 gctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc 721 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa 781 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg g //