LOCUS Exported 6461 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses Arabidopsis CIB1 (full length) fused to C-term fragment of Cre recombinase. ACCESSION . VERSION . KEYWORDS CIB1-CreC(N1) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6461) AUTHORS Taslimi A, Zoltowski B, Miranda JG, Pathak GP, Hughes RM, Tucker CL TITLE Optimized second-generation CRY2-CIB dimerizers and photoactivatable Cre recombinase. JOURNAL Nat Chem Biol. 2016 Apr 11. doi: 10.1038/nchembio.2063. PUBMED 27065233 REFERENCE 2 (bases 1 to 6461) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6461 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 61..364 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 365..568 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 519..539 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" CDS 628..1137 /codon_start=1 /product="N-terminal portion of the CIB1 transcription factor from Arabidopsis thaliana" /label=CIBN /note="binds to CRY2 (cryptochrome 2) that has absorbed blue light (Kennedy et al., 2010)" /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKKRKFDTETKDCN EKKKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE LEKTDYI" regulatory 2412..2421 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 2418..3128 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(2563..2581) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 2831..2850 /label=mCherry-F /note="mCherry, forward primer" polyA_signal 3249..3370 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3286..3305) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3340..3359 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(3377..3832) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3514..3535) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 3726..3745 /label=F1ori-R /note="F1 origin, reverse primer" promoter 3859..3963 /gene="bla" /label=AmpR promoter promoter 3965..4322 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(3988..4008) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 4173..4308 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4235..4254 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 4357..5151 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(4411..4430) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 5021..5040 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(5339..5358) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 5383..5430 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 5759..6347 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6248..6267 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct cgccaccatg aatggagcta taggaggtga ccttttgctc 661 aattttcctg acatgtcggt cctagagcgc caaagggctc acctcaagta cctcaatccc 721 acctttgatt ctcctctcgc cggcttcttt gccgattctt caatgattac cggcggcgag 781 atggacagct atctttcgac tgccggtttg aatcttccga tgatgtacgg tgagacgacg 841 gtggaaggtg attcaagact ctcaatttcg ccggaaacga cgcttgggac tggaaatttc 901 aagaaacgga agtttgatac agagactaag gattgtaatg agaagaagaa gaagatgacg 961 atgaacagag atgacctagt agaagaagga gaagaagaga agtcgaaaat aacagagcaa 1021 aacaatggga gcacaaaaag catcaagaag atgaaacaca aagccaagaa agaagagaac 1081 aatttctcta atgattcatc taaagtgacg aaggaattgg agaaaacgga ttatattcat 1141 gttcgtgcac gacgaggcca agccactgat agtcacagca tagcagaacg agttagaaga 1201 gaaaagatca gtgagagaat gaagtttcta caagatttgg ttcctggatg cgacaagatc 1261 acaggcaaag cagggatgct tgatgaaatc attaactatg ttcagtctct tcagagacaa 1321 atcgagttct tatcgatgaa actagcaatt gtgaatccaa ggccggattt tgatatggat 1381 gacatttttg ccaaagaggt tgcctcaact ccaatgactg tggtgccatc tcctgaaatg 1441 gttctttccg gttattctca tgagatggtt cactctggtt attctagtga gatggttaac 1501 tccggttacc ttcatgtcaa tccaatgcag caagtgaata ccagttctga tccattgtca 1561 tgcttcaaca atggcgaagc tccttcgatg tgggactctc atgtgcagaa tctctatggc 1621 aatttaggag ttggtggcgg tggctctgga ggtggtgggt ccggaggagg cggccgccga 1681 ccaagtgaca gcaatgctgt ttcactggtt atgcggcgga tccgaaaaga aaacgttgat 1741 gccggtgaac gtgcaaaaca ggctctagcg ttcgaacgca ctgatttcga ccaggttcgt 1801 tcactcatgg aaaatagcga tcgctgccag gatatacgta atctggcatt tctggggatt 1861 gcttataaca ccctgttacg tatagccgaa attgccagga tcagggttaa agatatctca 1921 cgtactgacg gtgggagaat gttaatccat attggcagaa cgaaaacgct ggttagcacc 1981 gcaggtgtag agaaggcact tagcctgggg gtaactaaac tggtcgagcg atggatttcc 2041 gtctctggtg tagctgatga tccgaataac tacctgtttt gccgggtcag aaaaaatggt 2101 gttgccgcgc catctgccac cagccagcta tcaactcgcg ccctggaagg gatttttgaa 2161 gcaactcatc gattgattta cggcgctaag gatgactctg gtcagagata cctggcctgg 2221 tctggacaca gtgcccgtgt cggagccgcg cgagatatgg cccgcgctgg agtttcaata 2281 ccggagatca tgcaagctgg tggctggacc aatgtaaata ttgtcatgaa ctatatccgt 2341 aacctggata gtgaaacagg ggcaatggtg cgcctgctgg aagatggcga ttagcccggg 2401 atccaccggt cgccaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg 2461 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg 2521 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca 2581 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca 2641 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg 2701 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg 2761 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc 2821 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga 2881 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg 2941 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc 3001 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca 3061 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt 3121 acaagtagcg gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac 3181 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg 3241 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 3301 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 3361 atgtatctta aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt 3421 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca 3481 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta 3541 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta 3601 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg 3661 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga 3721 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg 3781 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc 3841 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat 3901 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag 3961 agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc 4021 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt 4081 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 4141 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg 4201 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 4261 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca 4321 aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac 4381 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca 4441 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt 4501 gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg 4561 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga 4621 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct 4681 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg 4741 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg 4801 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc 4861 gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat 4921 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac 4981 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt 5041 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct 5101 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc 5161 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca 5221 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga 5281 tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg 5341 aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga 5401 ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc 5461 actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt 5521 ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg 5581 caggccctgc catagcctca ggttactcat atatacttta gattgattta aaacttcatt 5641 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 5701 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 5761 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 5821 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 5881 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca 5941 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 6001 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 6061 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 6121 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 6181 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 6241 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 6301 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 6361 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 6421 tatcccctga ttctgtggat aaccgtatta ccgccatgca t //