LOCUS Exported 962 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION a plasmid derived from pKHR5: in addition to Venus reporter gene, it also contains cmlc2::mCherry (red heart) reporter gene at 3' end of the mcs as pKHR7. Genbank accession number is KU144825.. ACCESSION . VERSION . KEYWORDS pKHR8 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 962) AUTHORS Hoshijima K, Jurynec MJ, Grunwald DJ TITLE Precise Editing of the Zebrafish Genome Made Simple and Efficient. JOURNAL Dev Cell. 2016 Mar 21;36(6):654-67. doi: 10.1016/j.devcel.2016.02.015. PUBMED 27003937 REFERENCE 2 (bases 1 to 962) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..962 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 2..21 /label=T7 /note="T7 promoter, forward primer" promoter 2..20 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind 102..118 /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind 102..118 /label=pBluescriptSK /note="For pBluescript vector" protein_bind 138..171 /label=FRT (minimal) /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="supports FLP-mediated excision but not integration (Turan and Bode, 2011)" polyA_signal 235..369 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(272..291) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 326..345 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" polyA_signal 406..613 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind 602..619 /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" CDS complement(640..891) /codon_start=1 /product="C-terminal fragment of mVenus for use in bimolecular fluorescence complementation (BiFC) (Kodama and Hu, 2010)" /label=VC155 /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK" primer_bind complement(682..703) /label=EGFP-C /note="EGFP, forward primer" ORIGIN 1 gtaatacgac tcactatagg gcgaattgga gctccaccgc ggtggcggcc aggtccttta 61 gtcgggtatt cgggtaggga taacagggta atggcggccg ctctagaact agtggatccc 121 ccgggctgca ggaattcgaa gttcctattc tctagaaagt ataggaactt cttaaaaaac 181 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg 241 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa 301 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat 361 gtctggatca tcatcgatgg tacccaccgc ggtggcggcg atctctcccc agcatgcctg 421 ctattgtctt cccaatcctc ccccttgctg tcctgcccca ccccaccccc cagaatagaa 481 tgacacctac tcagacaatg cgatgcaatt tcctcatttt attaggaaag gacagtggga 541 gtggcacctt ccagggtcaa ggaaggcacg ggggaggggc aaacaacaga tggctggcaa 601 ctagaaggca cagtcgaggc tgatcagcgg gccgctttac ttgtacagct cgtccatgcc 661 gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt 721 ggggtctttg ctcagggcgg actggtagct caggtagtgg ttgtcgggca gcagcacggg 781 gccgtcgccg atgggggtgt tctgctggta gtggtcggcg agctgcacgc cgccgtcctc 841 gatgttgtgg cggatcttga agttggcctt gatgccgttc ttctgcttgt cggcggtgat 901 atagacgttg tggctgttgt agttgtactc cagcttgtgc cccaggatgt tgccgtcctc 961 ct //