LOCUS       Exported                 884 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  a plasmid derived from pKHR5: in addition to Venus reporter gene, it
            also contains cmlc2::mCherry (red heart) reporter gene at 3' end of 
            the mcs as pKHR7. Genbank accession number is KU144825..
ACCESSION   .
VERSION     .
KEYWORDS    pKHR8
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Hoshijima K, Jurynec MJ, Grunwald DJ
  TITLE     Precise Editing of the Zebrafish Genome Made Simple and Efficient.
  JOURNAL   Dev Cell. 2016 Mar 21;36(6):654-67. doi: 
            10.1016/j.devcel.2016.02.015.
  PUBMED    27003937
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        25..43
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    173..307
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(210..229)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     264..283
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        312..329
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(425..448)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(623..642)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tgaccatgat tacgccaagc tcgaaattaa ccctcactaa agggaacaaa agctggtacc
       61 ggtcctttag tcgggtattc gggtagggat aacagggtaa tggcggctcg aaaaaaacct
      121 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
      181 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
      241 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
      301 ctggatctac gtaatacgac tcactatagt tctagaggcg cgccttactt gtacagctcg
      361 tccatgccgc cggtggagtg gcggccctcg gcgcgctcgt actgttccac gatggtgtag
      421 tcctcgttgt gggaggtgat gtccagcttg atgttgacgt tgtaggcgcc gggcagctgc
      481 acgggcttct tggccttgta ggtggtcttg acctcggcgt cgtagtggcc gccgtccttc
      541 agcttcagcc tctgcttgat ctcgcccttc agggcgccgt cctcggggta catccgctcg
      601 gaggaggcct cccagcccat ggtcttcttc tgcattacgg ggccgtcgga ggggaagttg
      661 gtgccgcgca gcttcacctt gtagatgaac tcgccgtcct gcagggagga gtcctgggtc
      721 acggtcacca cgccgccgtc ctcgaagttc atcacgcgct cccacttgaa gccctcgggg
      781 aaggacagct tcaagtagtc ggggatgtcg gcggggtgct tcacgtaggc cttggagccg
      841 tacatgaact gaggggacag gatgtcccag gcgaagggca gggg
//
