LOCUS       Exported                5151 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Luciferase reporter construct for human ACE2 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    ACE2(-252)-luc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Senkel S, Lucas B, Klein-Hitpass L, Ryffel GU
  TITLE     Identification of target genes of the transcription factor HNF1beta 
            and HNF1alpha in a human embryonic kidney cell line.
  JOURNAL   Biochim Biophys Acta. 2005 Dec 20. 1731(3):179-90.
  PUBMED    16297991
REFERENCE   2  (bases 1 to 5151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      414..423
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             420..2072
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(486..504)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    2113..2234
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2124..2143)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2178..2197
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2482..2499)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2653..3241)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2733..2752)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3412..4272)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4035..4054
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4273..4377)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4404..4859
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4491..4510)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4701..4722
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    4990..5038
                     /note="synthetic polyadenylation signal"
     misc_feature    5052..5143
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5092..5111
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggtaccgagc tcattttccg tgtatcttta acagctttct aggaaaatat taaccaaaag
       61 taccggtttt gatttggcca taaagtgaca ggagaggtaa ggttctctag gattaaagaa
      121 taacgtattc ttatttgatt cactttaaaa aattattcta aaatctgtta catatctgtc
      181 ctctccagga tgaactttat attggctcag cagattgttt actgtgttct tcttcttttt
      241 ctttttttgg tctttcctgc tcagcgccca acccaagttc aaaggctgat aagagagaaa
      301 atctcatgag gaggttttag tctagggaaa gtcattcagt ggatgtgatc ttggctcaca
      361 ggggacgaga tctgcgatct aagtaagctt ggcattccgg tactgttggt aaagccacca
      421 tggaagacgc caaaaacata aagaaaggcc cggcgccatt ctatccgctg gaagatggaa
      481 ccgctggaga gcaactgcat aaggctatga agagatacgc cctggttcct ggaacaattg
      541 cttttacaga tgcacatatc gaggtggaca tcacttacgc tgagtacttc gaaatgtccg
      601 ttcggttggc agaagctatg aaacgatatg ggctgaatac aaatcacaga atcgtcgtat
      661 gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg cgcgttattt atcggagttg
      721 cagttgcgcc cgcgaacgac atttataatg aacgtgaatt gctcaacagt atgggcattt
      781 cgcagcctac cgtggtgttc gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa
      841 aaaagctccc aatcatccaa aaaattatta tcatggattc taaaacggat taccagggat
      901 ttcagtcgat gtacacgttc gtcacatctc atctacctcc cggttttaat gaatacgatt
      961 ttgtgccaga gtccttcgat agggacaaga caattgcact gatcatgaac tcctctggat
     1021 ctactggtct gcctaaaggt gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc
     1081 atgccagaga tcctattttt ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg
     1141 ttccattcca tcacggtttt ggaatgttta ctacactcgg atatttgata tgtggatttc
     1201 gagtcgtctt aatgtataga tttgaagaag agctgtttct gaggagcctt caggattaca
     1261 agattcaaag tgcgctgctg gtgccaaccc tattctcctt cttcgccaaa agcactctga
     1321 ttgacaaata cgatttatct aatttacacg aaattgcttc tggtggcgct cccctctcta
     1381 aggaagtcgg ggaagcggtt gccaagaggt tccatctgcc aggtatcagg caaggatatg
     1441 ggctcactga gactacatca gctattctga ttacacccga gggggatgat aaaccgggcg
     1501 cggtcggtaa agttgttcca ttttttgaag cgaaggttgt ggatctggat accgggaaaa
     1561 cgctgggcgt taatcaaaga ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt
     1621 atgtaaacaa tccggaagcg accaacgcct tgattgacaa ggatggatgg ctacattctg
     1681 gagacatagc ttactgggac gaagacgaac acttcttcat cgttgaccgc ctgaagtctc
     1741 tgattaagta caaaggctat caggtggctc ccgctgaatt ggaatccatc ttgctccaac
     1801 accccaacat cttcgacgca ggtgtcgcag gtcttcccga cgatgacgcc ggtgaacttc
     1861 ccgccgccgt tgttgttttg gagcacggaa agacgatgac ggaaaaagag atcgtggatt
     1921 acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg
     1981 aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa aatcagagag atcctcataa
     2041 aggccaagaa gggcggaaag atcgccgtgt aattctagag tcggggcggc cggccgcttc
     2101 gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     2161 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     2221 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     2281 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc
     2341 cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
     2401 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
     2461 ccggcagcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     2521 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     2581 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     2641 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2701 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2761 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2821 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2881 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2941 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     3001 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     3061 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     3121 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     3181 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     3241 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     3301 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     3361 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     3421 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     3481 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     3541 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     3601 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     3661 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     3721 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     3781 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     3841 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     3901 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     3961 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     4021 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     4081 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     4141 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     4201 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     4261 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     4321 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     4381 atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     4441 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     4501 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     4561 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     4621 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     4681 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     4741 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     4801 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttg
     4861 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     4921 attacgccag cccaagctac catgataagt aagtaatatt aaggtacggg aggtacttgg
     4981 agcggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa
     5041 tcgatagtac taacatacgc tctccatcaa aacaaaacga aacaaaacaa actagcaaaa
     5101 taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc tctatcgata a
//
