LOCUS       Exported                5102 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Luciferase reporter construct for human ACE2 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    ACE2(-202)-luc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5102)
  AUTHORS   Senkel S, Lucas B, Klein-Hitpass L, Ryffel GU
  TITLE     Identification of target genes of the transcription factor HNF1beta 
            and HNF1alpha in a human embryonic kidney cell line.
  JOURNAL   Biochim Biophys Acta. 2005 Dec 20. 1731(3):179-90.
  PUBMED    16297991
REFERENCE   2  (bases 1 to 5102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5102
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      365..374
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             371..2023
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(437..455)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    2064..2185
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2075..2094)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2129..2148
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2433..2450)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2604..3192)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2684..2703)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3363..4223)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3986..4005
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4224..4328)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4355..4810
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4442..4461)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4652..4673
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    4941..4989
                     /note="synthetic polyadenylation signal"
     misc_feature    5003..5094
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5043..5062
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggtaccgagc tcaccggttt tgatttggcc ataaagtgac aggagaggta aggttctcta
       61 ggattaaaga ataacgtatt cttatttgat tcactttaaa aaattattct aaaatctgtt
      121 acatatctgt cctctccagg atgaacttta tattggctca gcagattgtt tactgtgttc
      181 ttcttctttt tctttttttg gtctttcctg ctcagcgccc aacccaagtt caaaggctga
      241 taagagagaa aatctcatga ggaggtttta gtctagggaa agtcattcag tggatgtgat
      301 cttggctcac aggggacgag atctgcgatc taagtaagct tggcattccg gtactgttgg
      361 taaagccacc atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatccgct
      421 ggaagatgga accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc
      481 tggaacaatt gcttttacag atgcacatat cgaggtggac atcacttacg ctgagtactt
      541 cgaaatgtcc gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag
      601 aatcgtcgta tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt
      661 tatcggagtt gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag
      721 tatgggcatt tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc aaaaaatttt
      781 gaacgtgcaa aaaaagctcc caatcatcca aaaaattatt atcatggatt ctaaaacgga
      841 ttaccaggga tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa
      901 tgaatacgat tttgtgccag agtccttcga tagggacaag acaattgcac tgatcatgaa
      961 ctcctctgga tctactggtc tgcctaaagg tgtcgctctg cctcatagaa ctgcctgcgt
     1021 gagattctcg catgccagag atcctatttt tggcaatcaa atcattccgg atactgcgat
     1081 tttaagtgtt gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat
     1141 atgtggattt cgagtcgtct taatgtatag atttgaagaa gagctgtttc tgaggagcct
     1201 tcaggattac aagattcaaa gtgcgctgct ggtgccaacc ctattctcct tcttcgccaa
     1261 aagcactctg attgacaaat acgatttatc taatttacac gaaattgctt ctggtggcgc
     1321 tcccctctct aaggaagtcg gggaagcggt tgccaagagg ttccatctgc caggtatcag
     1381 gcaaggatat gggctcactg agactacatc agctattctg attacacccg agggggatga
     1441 taaaccgggc gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga
     1501 taccgggaaa acgctgggcg ttaatcaaag aggcgaactg tgtgtgagag gtcctatgat
     1561 tatgtccggt tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg
     1621 gctacattct ggagacatag cttactggga cgaagacgaa cacttcttca tcgttgaccg
     1681 cctgaagtct ctgattaagt acaaaggcta tcaggtggct cccgctgaat tggaatccat
     1741 cttgctccaa caccccaaca tcttcgacgc aggtgtcgca ggtcttcccg acgatgacgc
     1801 cggtgaactt cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga
     1861 gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt
     1921 gtttgtggac gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga
     1981 gatcctcata aaggccaaga agggcggaaa gatcgccgtg taattctaga gtcggggcgg
     2041 ccggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag
     2101 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     2161 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     2221 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat
     2281 cgataaggat ccgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt
     2341 gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg
     2401 taggacaggt gccggcagcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     2461 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     2521 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     2581 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     2641 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2701 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2761 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2821 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2881 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2941 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     3001 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     3061 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     3121 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     3181 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     3241 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     3301 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     3361 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     3421 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     3481 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     3541 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     3601 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     3661 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     3721 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     3781 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     3841 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     3901 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     3961 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     4021 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     4081 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     4141 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     4201 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     4261 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     4321 gttccgcgca catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc
     4381 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     4441 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     4501 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     4561 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     4621 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     4681 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     4741 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     4801 cttacaattt gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     4861 gcctcttcgc tattacgcca gcccaagcta ccatgataag taagtaatat taaggtacgg
     4921 gaggtacttg gagcggccgc aataaaatat ctttattttc attacatctg tgtgttggtt
     4981 ttttgtgtga atcgatagta ctaacatacg ctctccatca aaacaaaacg aaacaaaaca
     5041 aactagcaaa ataggctgtc cccagtgcaa gtgcaggtgc cagaacattt ctctatcgat
     5101 aa
//
