LOCUS       Exported                 862 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  Lentiviral expression of WT-STAT1.
ACCESSION   .
VERSION     .
KEYWORDS    pLV-WT-STAT1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Cheon H, Stark GR
  TITLE     Unphosphorylated STAT1 prolongs the expression of interferon-induced
            immune regulatory genes.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Jun 9;106(23):9373-8. doi: 
            10.1073/pnas.0903487106. Epub 2009 May 28.
  PUBMED    19478064
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        24..75
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     26..46
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     120..139
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        120..138
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
       61 tatataagca gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt
      121 aatacgactc actataggga gacccaagct tggtaccgag ctcggatcca ctagtaacgg
      181 ccgccagtgt gctggaattc tgcagatatc catcacactg gcggccgcac aaggtggcag
      241 gatgtctcag tggtacgaac ttcagcagct tgactcaaaa ttcctggagc aggttcacca
      301 gctttatgat gacagttttc ccatggaaat cagacagtac ctggcacagt ggttagaaaa
      361 gcaagactgg gagcacgctg ccaatgatgt ttcatttgcc accatccgtt ttcatgacct
      421 cctgtcacag ctggatgatc aatatagtcg cttttctttg gagaataact tcttgctaca
      481 gcataacata aggaaaagca agcgtaatct tcaggataat tttcaggaag acccaatcca
      541 gatgtctatg atcatttaca gctgtctgaa ggaagaaagg aaaattctgg aaaacgccca
      601 gagatttaat caggctcagt cggggaatat tcagagcaca gtgatgttag acaaacagaa
      661 agagcttgac agtaaagtca gaaatgtgaa ggacaaggtt atgtgtatag agcatgaaat
      721 caagagcctg gaagatttac aagatgaata tgacttcaaa tgcaaaacct tgcagaacag
      781 agaacacgag accaatggtg tggcaaagag tgatcagaaa caagaacagc tgttactcaa
      841 gaagatgtat ttaatgcttg ac
//
