LOCUS       Exported               14231 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pDEST-HemmarG
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14231)
  AUTHORS   Han C, Jan LY, Jan YN
  TITLE     Enhancer-driven membrane markers for analysis of nonautonomous 
            mechanisms reveal neuron-glia interactions in Drosophila.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 May 23. ():.
  PUBMED    21606367
REFERENCE   2  (bases 1 to 14231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14231
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..233
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    631..1016
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, and
                     Mod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    1050..1174
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1199..1229
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1283..1942
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1349..1368)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2284..2589
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2468..2487
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2630..2754)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3897..4412
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIE"
     primer_bind     complement(3939..3960)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4200..4219)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4547..4568
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4659..5372
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label=GFP
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELY"
     primer_bind     complement(4692..4720)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     5315..5335
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    6085..6470
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, and
                     Mod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     primer_bind     complement(6480..6498)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(6950..7021,7423..7696,7771..8425,8487..8802,9006..9137,
                     9208..9822)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    10601..11186
                     /label=P element 5' end
     rep_origin      complement(11421..12009)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11501..11520)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12180..13040)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12803..12822
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13041..13145)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     13213..13231
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(13269..13291)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     13391..13410
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    13619..13688
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
       61 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
      121 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
      181 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttc
      241 ctaggtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
      301 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
      361 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
      421 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
      481 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
      541 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
      601 agccgaactg ttcgccaagc ttggctgcat cacgtaataa gtgtgcgttg aatttattcg
      661 caaaaacatt gcatattttc ggcaaagtaa aattttgttg cataccttat caaaaaataa
      721 gtgctgcata ctttttagag aaaccaaata attttttatt gcatacccgt ttttaataaa
      781 atacattgca taccctcttt taataaaaaa tattgcatac tttgacgaaa caaattttcg
      841 ttgcataccc aataaaagat tattatattg catacccgtt tttaataaaa tacattgcat
      901 accctctttt aataaaaaat attgcatacg ttgacgaaac aaattttcgt tgcataccca
      961 ataaaagatt attatattgc ataccttttc ttgccatacc atttagccga tcaattgtgc
     1021 tcggcaacag catgctgcag cagatcatca caagtttgta caaaaaagct gaacgagaaa
     1081 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
     1141 aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt
     1201 tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc cgtcgagatt
     1261 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
     1321 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
     1381 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
     1441 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
     1501 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
     1561 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
     1621 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
     1681 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
     1741 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
     1801 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
     1861 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
     1921 tgagtggcag ggcggggcgt aaacgcgtgg atccggctta ctaaaagcca gataacagta
     1981 tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag
     2041 tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc
     2101 tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc
     2161 agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg
     2221 ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac aggggctggt
     2281 gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat
     2341 gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca
     2401 cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa
     2461 agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa
     2521 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
     2581 ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg
     2641 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
     2701 attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggtgatgat
     2761 ctggtctagc gagcgccgga gtataaatag aggcgcttcg tctacggagc gacaattcaa
     2821 ttcaaacaag caaagtgaac acgtcgctaa gcgaaagcta agcaaataaa caagcgcagc
     2881 tgagcaagct aaacaatcgt aaagtgcaag ttaaagtgaa tcaattaaaa gtaaccagca
     2941 accaagtaaa tcaactgcaa ctactgaaat ctgccggtga gattgtacac ccttcttaaa
     3001 gatccccttc aaagtctaaa ttcttccttt tcgtttagcc aagactcgag agaaaaccgg
     3061 tacgcgtgaa ttccaaaatg aatcccaaga gcgaagtcct cattgcagcc gtgctgttca
     3121 tgctgctggc ctgcgtccag tgtcaagcta gcggtggcgg cggaagtgga ggtggaggct
     3181 cgttccagaa ggcctccagc atagtctata agaaagaggg ggaacaggtg gagttctcct
     3241 tcccactcgc ctttacagtt gaaaagctga cgggcagtgg cgagctgtgg tggcaggcgg
     3301 agagggcttc ctcctccaag tcttggatca cctttgacct gaagaacaag gaagtgtctg
     3361 taaaacgggt tacccaggac cctaagctcc agatgggcaa gaagctcccg ctccacctca
     3421 ccctgcccca ggccttgcct cagtatgctg gctctggaaa cctcaccctg gcccttgaag
     3481 cgaaaacagg aaagttgcat caggaagtga acctggtggt gatgagagcc actcagctcc
     3541 agaaaaattt gacctgtgag gtgtggggac ccacctcccc taagctgatg ctgagcttga
     3601 aactggagaa caaggaggca aaggtctcga agcgggagaa ggcggtgtgg gtgctgaacc
     3661 ctgaggcggg gatgtggcag tgtctgctga gtgactcggg acaggtcctg ctggaatcca
     3721 acatcaaggt tctgcccaca tggtccaccc cggtgcagcc aatggccctg attgtgctgg
     3781 ggggcgtcgc cggcctcctg cttttcattg ggctaggcat cttcttctgt gtcaggtgcc
     3841 ggcaccgaag gcgcggcgga agcggaggcg gatcccgtag tcgtgcccag gcttccgtga
     3901 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     3961 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     4021 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     4081 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     4141 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     4201 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     4261 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     4321 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     4381 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     4441 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     4501 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     4561 agttcgtgac cgccgccggg atcactctcg gcatggggca tggcaccggc agcaccggca
     4621 gcggcagctc cggaaccgcc tcctccgagg acaacaacat ggccagtaaa ggagaagaac
     4681 ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat
     4741 tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta
     4801 tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact ttaacttatg
     4861 gtgttcaatg cttttcaaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg
     4921 ccatgcccga aggttatgtc caggaaagaa ctatattttt caaagatgac gggaactaca
     4981 agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag
     5041 gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac aactataact
     5101 cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa
     5161 ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc
     5221 caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc
     5281 tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg
     5341 ctgggattac acatggcatg gatgaactct acgagaacgc aaacagcttt tgctatgaaa
     5401 atgaagttgc cctctaatct agaattaatt aactaagcca gtcggcaatc ggacgccttc
     5461 gaaacatgca acactaatgt ttaattcaga tttcagcaga gacaagctaa aacaccgacg
     5521 agttgtaatc atttctgtgc gccagcatat atttcttata tacaacgtaa tacataatta
     5581 tgtaattcta gcatctcccc aacactcaca tacaaacaaa caaaaaatac aaacacacaa
     5641 aacgtattta cccgcacgca tccttggcga ggttgagtat gaaacaaaaa caaaacttaa
     5701 tttagagcaa agtaattaca cgaataaatt taataaaaaa aaactataat aaaaacgcca
     5761 atcatgttat ttccaaaaaa aaaaaaaaaa aaaaaactag acgagatgtt tgcgcttgtg
     5821 tcgatgaatg taaagaattg caactacata gatggtaatt aatttcagct gtaagaagta
     5881 caagtttttc ataattgtga gaaaagtgca gtagtatcgc ttaaagttca gttttaatca
     5941 gggcatttag cagctcgaac ccaatgtatt tgtaagccca ataaacgtgt aaatgcctag
     6001 agcaaaatat cacaactgta gcttttcatc cgaaagataa taatgaatag actgacctga
     6061 cgtttttcaa aatgcgtaac tagtcacgta ataagtgtgc gttgaattta ttcgcaaaaa
     6121 cattgcatat tttcggcaaa gtaaaatttt gttgcatacc ttatcaaaaa ataagtgctg
     6181 catacttttt agagaaacca aataattttt tattgcatac ccgtttttaa taaaatacat
     6241 tgcataccct cttttaataa aaaatattgc atactttgac gaaacaaatt ttcgttgcat
     6301 acccaataaa agattattat attgcatacc cgtttttaat aaaatacatt gcataccctc
     6361 ttttaataaa aaatattgca tacgttgacg aaacaaattt tcgttgcata cccaataaaa
     6421 gattattata ttgcatacct tttcttgcca taccatttag ccgatcaatt ctagtatgta
     6481 tgtaagttaa taaaacccat ttttgcggaa agtagataaa aaaaacattt ttttttttta
     6541 ctgcactgga tatcattgaa cttatctgat cagttttaaa tttacttcga tccaagggta
     6601 tttgatgtac caggttcttt cgattacctc tcactcaaaa tgacattcca ctcaaagtca
     6661 gcgctgtttg cctccttctc tgtccacaga aatatcgccg tctctttcgc cgctgcgtcc
     6721 gctatctctt tcgccaccgt ttgtagcgtt acgtagcgtc aatgtccgcc ttcagttgca
     6781 ttttgtcagc ggtttcgtga cgaagctcca agcggtttac gccatcaatt aaacacaaag
     6841 tgctgtgcca aaactcctct cgcttcttat ttttgtttgt tttttgagtg attggggtgg
     6901 tgattggttt tgggtgggta agcaggggaa agtgtgaaaa atcccggcaa tgggccaaga
     6961 ggatcaggag ctattaattc gcggaggcag caaacaccca tctgccgagc atctgaacaa
     7021 tgtgagtagt acatgtgcat acatcttaag ttcacttgat ctataggaac tgcgattgca
     7081 acatcaaatt gtctgcggcg tgagaactgc gacccacaaa aatcccaaac cgcaatcgca
     7141 caaacaaata gtgacacgaa acagattatt ctggtagctg ttctcgctat ataagacaat
     7201 ttttgagatc atatcatgat caagacatct aaaggcattc attttcgact atattctttt
     7261 ttacaaaaaa tataacaacc agatatttta agctgatcct agatgcacaa aaaataaata
     7321 aaagtataaa cctacttcgt aggatacttc ggggtacttt ttgttcgggg ttagatgagc
     7381 ataacgcttg tagttgatat ttgagatccc ctatcattgc agggtgacag cggagcggct
     7441 tcgcagagct gcattaacca gggcttcggg caggccaaaa actacggcac gctccggcca
     7501 cccagtccgc cggaggactc cggttcaggg agcggccaac tagccgagaa cctcacctat
     7561 gcctggcaca atatggacat ctttggggcg gtcaatcagc cgggctccgg atggcggcag
     7621 ctggtcaacc ggacacgcgg actattctgc aacgagcgac acataccggc gcccaggaaa
     7681 catttgctca agaacggtga gtttctattc gcagtcggct gatctgtgtg aaatcttaat
     7741 aaagggtcca attaccaatt tgaaactcag tttgcggcgt ggcctatccg ggcgaacttt
     7801 tggccgtgat gggcagttcc ggtgccggaa agacgaccct gctgaatgcc cttgcctttc
     7861 gatcgccgca gggcatccaa gtatcgccat ccgggatgcg actgctcaat ggccaacctg
     7921 tggacgccaa ggagatgcag gccaggtgcg cctatgtcca gcaggatgac ctctttatcg
     7981 gctccctaac ggccagggaa cacctgattt tccaagccat ggtgcggatg ccacgacatc
     8041 tgacctatcg gcagcgagtg gcccgcgtgg atcaggtgat ccaggagctt tcgctcagca
     8101 aatgtcagca cacgatcatc ggtgtgcccg gcagggtgaa aggtctgtcc ggcggagaaa
     8161 ggaagcgtct ggcattcgcc tccgaggctc taaccgatcc gccgcttctg atctgcgatg
     8221 agcccacctc cggactggac tcctttaccg cccacagcgt cgtccaggtg ctgaagaagc
     8281 tgtcgcagaa gggcaagacc gtcatcctga ccattcatca gccgtcttcc gagctgtttg
     8341 agctctttga caagatcctt ctgatggccg agggcagggt agctttcttg ggcactccca
     8401 gcgaagccgt cgacttcttt tcctagtgag ttcgatgtgt ttattaaggg tatctagtat
     8461 tacataacat ctcaactcct atccagcgtg ggtgcccagt gtcctaccaa ctacaatccg
     8521 gcggactttt acgtacaggt gttggccgtt gtgcccggac gggagatcga gtcccgtgat
     8581 cggatcgcca agatatgcga caattttgcc attagcaaag tagcccggga tatggagcag
     8641 ttgttggcca ccaaaaatct ggagaagcca ctggagcagc cggagaatgg gtacacctac
     8701 aaggccacct ggttcatgca gttccgggcg gtcctgtggc gatcctggct gtcggtgctc
     8761 aaggaaccac tcctcgtaaa agtgcgactt attcagacaa cggtgagtgg ttccagtgga
     8821 aacaaatgat ataacgctta caattcttgg aaacaaattc gctagatttt agatagaatt
     8881 gcctgattcc acacccttct tagttttttt caatgagatg tatagtttat agttttgcag
     8941 aagataaata aatttcattt aactcgcgaa tattaatgag atgcgagtaa cattttaatt
     9001 tgcagatggt tgccatcttg attggcctca tctttttggg ccaacaactc acgcaagtgg
     9061 gtgtgatgaa tatcaacgga gccatcttcc tcttcctgac caacatgacc tttcaaaacg
     9121 tctttgccac gataaatgta agtcatgttt agaatacatt tgcatttcaa taatttacta
     9181 actttctaat gaatcgattc gatttaggtg ttcacctcag agctgccagt ttttatgagg
     9241 gaggcccgaa gtcgacttta tcgctgtgac acatactttc tgggcaaaac gattgccgaa
     9301 ttgccgcttt ttctcacagt gccactggtc ttcacggcga ttgcctatcc gatgatcgga
     9361 ctgcgggccg gagtgctgca cttcttcaac tgcctggcgc tggtcactct ggtggccaat
     9421 gtgtcaacgt ccttcggata tctaatatcc tgcgccagct cctcgacctc gatggcgctg
     9481 tctgtgggtc cgccggttat cataccattc ctgctctttg gcggcttctt cttgaactcg
     9541 ggctcggtgc cagtatacct caaatggttg tcgtacctct catggttccg ttacgccaac
     9601 gagggtctgc tgattaacca atgggcggac gtggagccgg gcgaaattag ctgcacatcg
     9661 tcgaacacca cgtgccccag ttcgggcaag gtcatcctgg agacgcttaa cttctccgcc
     9721 gccgatctgc cgctggacta cgtgggtctg gccattctca tcgtgagctt ccgggtgctc
     9781 gcatatctgg ctctaagact tcgggcccga cgcaaggagt agccgacata tatccgaaat
     9841 aactgcttgt tttttttttt taccattatt accatcgtgt ttactgttta ttgccccctc
     9901 aaaaagctaa tgtaattata tttgtgccaa taaaaacaag atatgaccta tagaatacaa
     9961 gtatttcccc ttcgaacatc cccacaagta gactttggat ttgtcttcta accaaaagac
    10021 ttacacacct gcatacctta catcaaaaac tcgtttatcg ctacataaaa caccgggata
    10081 tattttttat atacatactt ttcaaatcgc gcgccctctt cataattcac ctccaccaca
    10141 ccacgtttcg tagttgctct ttcgctgtct cccacccgct ctccgcaaca cattcacctt
    10201 ttgttcgacg accttggagc gactgtcgtt agttccgcgc gattcggttc gctcaaatgg
    10261 ttccgagtgg ttcatttcgt ctcaatagaa attagtaata aatatttgta tgtacaattt
    10321 atttgctcca atatatttgt atatatttcc ctcacagcta tatttattct aatttaatat
    10381 tatgactttt taaggtaatt ttttgtgacc tgttcggagt gattagcgtt acaatttgaa
    10441 ctgaaagtga catccagtgt ttgttccttg tgtagatgca tctcaaaaaa atggtgggca
    10501 taatagtgtt gtttatatat atcaaaaata acaactataa taataagaat acatttaatt
    10561 tagaaaatgc ttggatttca ctggaactag aattaattcg gctgctgctc taaacgacgc
    10621 atttcgtact ccaaagtacg aattttttcc ctcaagctct tattttcatt aaacaatgaa
    10681 caggacctaa cgcacagtca cgttattgtt tacataaatg atttttttta ctattcaaac
    10741 ttactctgtt tgtgtactcc cactggtata gccttctttt atcttttctg gttcaggctc
    10801 tatcacttta ctaggtacgg catctgcgtt gagtcgcctc cttttaaatg tctgaccttt
    10861 tgcaggtgca gccttccact gcgaatcatt aaagtgggta tcacaaattt gggagttttc
    10921 accaaggctg cacccaaggc tctgctccca caattttctc ttaatagcac acttcggcac
    10981 gtgaattaat tttactccag tcacagcttt gcagcaaaat ttgcaatatt tcattttttt
    11041 ttattccacg taagggttaa tgttttcaaa aaaaaattcg tccgcacaca acctttcctc
    11101 tcaacaagca aacgtgcact gaatttaagt gtatacttcg gtaagcttcg gctatcgacg
    11161 ggaccacctt atgttatttc atcatgggcc agacccacgt agtccagcgg cagatcggcg
    11221 gcggagaagt taagcgtctc caggatgacc ttgcccgaac tggggcacgt ggtgttcgac
    11281 gatgtgcagc taatttcgcc cggctccacg tccgcccatt ggttaatcag cagaccctcg
    11341 ttggcgtaac ggaaccatga gaggtacgac aaccatttga ggtatactgg caccgagccc
    11401 gagttcaaga agaaggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
    11461 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    11521 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    11581 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
    11641 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    11701 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
    11761 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    11821 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
    11881 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    11941 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    12001 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    12061 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
    12121 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
    12181 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
    12241 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
    12301 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
    12361 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
    12421 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
    12481 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    12541 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    12601 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    12661 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    12721 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    12781 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    12841 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    12901 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    12961 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    13021 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    13081 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    13141 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
    13201 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
    13261 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
    13321 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
    13381 cttaactatg cggcatcaga gcagattgta ctgagagtgc accattcgac atgcccgccg
    13441 tgaccgtcga gaacccgctg acgctgcccc gcgtatccgc acccgccgac gccgtcgcac
    13501 gtcccgtgct caccgtgacc accgcgccca gcggtttcga gggcgagggc ttcccggtgc
    13561 gccgcgcgtt cgccgggatc aactaccgcc acctcgaccc gttcatcatg atggaccaga
    13621 tgggtgaggt ggagtacgcg cccggggagc ccaagggcac gccctggcac ccgcaccgcg
    13681 gcttcgagac cgtgacctac atcgtcgata tgcggtgtga aataccgcac cgaatcgcgc
    13741 ggaactaacg acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg
    13801 ggtgggagac agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga
    13861 gggcgcgcga tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat
    13921 aaacgagttt ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc
    13981 aaagtctact tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg
    14041 tttttattgg cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac
    14101 gatggtaata atggtaaaaa aaaaaaacaa gcagttattt cggatatatg tcggctactc
    14161 cttgcgtcgg gcccgaagtc ttagagccag atatgcgagc acccggaagc tcacgatgag
    14221 aatggccaga c
//
