LOCUS       Exported               14160 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pDEST-HemmarR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14160)
  AUTHORS   Han C, Jan LY, Jan YN
  TITLE     Enhancer-driven membrane markers for analysis of nonautonomous 
            mechanisms reveal neuron-glia interactions in Drosophila.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 May 23. ():.
  PUBMED    21606367
REFERENCE   2  (bases 1 to 14160)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14160
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..233
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    631..1016
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, and
                     Mod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    1050..1174
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1199..1229
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1283..1942
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1349..1368)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2284..2589
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2468..2487
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2630..2754)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3876..5303
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     protein_bind    6016..6401
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, and
                     Mod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     primer_bind     complement(6411..6429)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     misc_feature    10530..11115
                     /label=P element 5' end
     rep_origin      complement(11350..11938)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11430..11449)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12109..12969)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12732..12751
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12970..13074)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     13142..13160
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(13198..13220)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     13320..13339
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    13548..13617
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
       61 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
      121 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
      181 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttc
      241 ctaggtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
      301 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
      361 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
      421 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
      481 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
      541 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
      601 agccgaactg ttcgccaagc ttggctgcat cacgtaataa gtgtgcgttg aatttattcg
      661 caaaaacatt gcatattttc ggcaaagtaa aattttgttg cataccttat caaaaaataa
      721 gtgctgcata ctttttagag aaaccaaata attttttatt gcatacccgt ttttaataaa
      781 atacattgca taccctcttt taataaaaaa tattgcatac tttgacgaaa caaattttcg
      841 ttgcataccc aataaaagat tattatattg catacccgtt tttaataaaa tacattgcat
      901 accctctttt aataaaaaat attgcatacg ttgacgaaac aaattttcgt tgcataccca
      961 ataaaagatt attatattgc ataccttttc ttgccatacc atttagccga tcaattgtgc
     1021 tcggcaacag catgctgcag cagatcatca caagtttgta caaaaaagct gaacgagaaa
     1081 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
     1141 aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt
     1201 tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc cgtcgagatt
     1261 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
     1321 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
     1381 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
     1441 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
     1501 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
     1561 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
     1621 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
     1681 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
     1741 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
     1801 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
     1861 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
     1921 tgagtggcag ggcggggcgt aaacgcgtgg atccggctta ctaaaagcca gataacagta
     1981 tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag
     2041 tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc
     2101 tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc
     2161 agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg
     2221 ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac aggggctggt
     2281 gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat
     2341 gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca
     2401 cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa
     2461 agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa
     2521 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
     2581 ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg
     2641 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
     2701 attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggtgatgat
     2761 ctggtctagc gagcgccgga gtataaatag aggcgcttcg tctacggagc gacaattcaa
     2821 ttcaaacaag caaagtgaac acgtcgctaa gcgaaagcta agcaaataaa caagcgcagc
     2881 tgagcaagct aaacaatcgt aaagtgcaag ttaaagtgaa tcaattaaaa gtaaccagca
     2941 accaagtaaa tcaactgcaa ctactgaaat ctgccggtga gattgtacac ccttcttaaa
     3001 gatccccttc aaagtctaaa ttcttccttt tcgtttagcc aagactcgag agaaaaccgg
     3061 tacgcgtgaa ttccaaaatg aatcccaaga gcgaagtcct cattgcagcc gtgctgttca
     3121 tgctgctggc ctgcgtccag tgtcaagcta gcggtggcgg cggaagtgga ggtggaggct
     3181 cgttccagaa ggcctccagc atagtctata agaaagaggg ggaacaggtg gagttctcct
     3241 tcccactcgc ctttacagtt gaaaagctga cgggcagtgg cgagctgtgg tggcaggcgg
     3301 agagggcttc ctcctccaag tcttggatca cctttgacct gaagaacaag gaagtgtctg
     3361 taaaacgggt tacccaggac cctaagctcc agatgggcaa gaagctcccg ctccacctca
     3421 ccctgcccca ggccttgcct cagtatgctg gctctggaaa cctcaccctg gcccttgaag
     3481 cgaaaacagg aaagttgcat caggaagtga acctggtggt gatgagagcc actcagctcc
     3541 agaaaaattt gacctgtgag gtgtggggac ccacctcccc taagctgatg ctgagcttga
     3601 aactggagaa caaggaggca aaggtctcga agcgggagaa ggcggtgtgg gtgctgaacc
     3661 ctgaggcggg gatgtggcag tgtctgctga gtgactcggg acaggtcctg ctggaatcca
     3721 acatcaaggt tctgcccaca tggtccaccc cggtgcagcc aatggccctg attgtgctgg
     3781 ggggcgtcgc cggcctcctg cttttcattg ggctaggcat cttcttctgt gtcaggtgcc
     3841 ggcaccgaag gcgcggcgga agcggaggcg gatccatggt gagcaagggc gaggaggtca
     3901 tcaaagagtt catgcgcttc aaggtgcgca tggagggctc catgaacggc cacgagttcg
     3961 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
     4021 tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag ttcatgtacg
     4081 gctccaaggc gtacgtgaag caccccgccg acatccccga ttacaagaag ctgtccttcc
     4141 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggtctg gtgaccgtga
     4201 cccaggactc ctccctgcag gacggcacgc tgatctacaa ggtgaagatg cgcggcacca
     4261 acttcccccc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctccaccg
     4321 agcgcctgta cccccgcgac ggcgtgctga agggcgagat ccaccaggcc ctgaagctga
     4381 aggacggcgg ccactacctg gtggagttca agaccatcta catggccaag aagcccgtgc
     4441 aactgcccgg ctactactac gtggacacca agctggacat cacctcccac aacgaggact
     4501 acaccatcgt ggaacagtac gagcgctccg agggccgcca ccacctgttc ctggggcatg
     4561 gcaccggcag caccggcagc ggcagctccg gcaccgcctc ctccgaggac aacaacatgg
     4621 ccgtcatcaa agagttcatg cgcttcaagg tgcgcatgga gggctccatg aacggccacg
     4681 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
     4741 tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttca
     4801 tgtacggctc caaggcgtac gtgaagcacc ccgccgacat ccccgattac aagaagctgt
     4861 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggtctggtga
     4921 ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg
     4981 gcaccaactt cccccccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     5041 ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac caggccctga
     5101 agctgaagga cggcggccac tacctggtgg agttcaagac catctacatg gccaagaagc
     5161 ccgtgcaact gcccggctac tactacgtgg acaccaagct ggacatcacc tcccacaacg
     5221 aggactacac catcgtggaa cagtacgagc gctccgaggg ccgccaccac ctgttcctgt
     5281 acggcatgga cgagctgtac aaggagaacg caaacagctt ttgctatgaa aatgaagttg
     5341 ccctctaatc tagaattaat taactaagcc agtcggcaat cggacgcctt cgaaacatgc
     5401 aacactaatg tttaattcag atttcagcag agacaagcta aaacaccgac gagttgtaat
     5461 catttctgtg cgccagcata tatttcttat atacaacgta atacataatt atgtaattct
     5521 agcatctccc caacactcac atacaaacaa acaaaaaata caaacacaca aaacgtattt
     5581 acccgcacgc atccttggcg aggttgagta tgaaacaaaa acaaaactta atttagagca
     5641 aagtaattac acgaataaat ttaataaaaa aaaactataa taaaaacgcc aatcatgtta
     5701 tttccaaaaa aaaaaaaaaa aaaaaaacta gacgagatgt ttgcgcttgt gtcgatgaat
     5761 gtaaagaatt gcaactacat agatggtaat taatttcagc tgtaagaagt acaagttttt
     5821 cataattgtg agaaaagtgc agtagtatcg cttaaagttc agttttaatc agggcattta
     5881 gcagctcgaa cccaatgtat ttgtaagccc aataaacgtg taaatgccta gagcaaaata
     5941 tcacaactgt agcttttcat ccgaaagata ataatgaata gactgacctg acgtttttca
     6001 aaatgcgtaa ctagtcacgt aataagtgtg cgttgaattt attcgcaaaa acattgcata
     6061 ttttcggcaa agtaaaattt tgttgcatac cttatcaaaa aataagtgct gcatactttt
     6121 tagagaaacc aaataatttt ttattgcata cccgttttta ataaaataca ttgcataccc
     6181 tcttttaata aaaaatattg catactttga cgaaacaaat tttcgttgca tacccaataa
     6241 aagattatta tattgcatac ccgtttttaa taaaatacat tgcataccct cttttaataa
     6301 aaaatattgc atacgttgac gaaacaaatt ttcgttgcat acccaataaa agattattat
     6361 attgcatacc ttttcttgcc ataccattta gccgatcaat tctagtatgt atgtaagtta
     6421 ataaaaccca tttttgcgga aagtagataa aaaaaacatt tttttttttt actgcactgg
     6481 atatcattga acttatctga tcagttttaa atttacttcg atccaagggt atttgatgta
     6541 ccaggttctt tcgattacct ctcactcaaa atgacattcc actcaaagtc agcgctgttt
     6601 gcctccttct ctgtccacag aaatatcgcc gtctctttcg ccgctgcgtc cgctatctct
     6661 ttcgccaccg tttgtagcgt tacgtagcgt caatgtccgc cttcagttgc attttgtcag
     6721 cggtttcgtg acgaagctcc aagcggttta cgccatcaat taaacacaaa gtgctgtgcc
     6781 aaaactcctc tcgcttctta tttttgtttg ttttttgagt gattggggtg gtgattggtt
     6841 ttgggtgggt aagcagggga aagtgtgaaa aatcccggca atgggccaag aggatcagga
     6901 gctattaatt cgcggaggca gcaaacaccc atctgccgag catctgaaca atgtgagtag
     6961 tacatgtgca tacatcttaa gttcacttga tctataggaa ctgcgattgc aacatcaaat
     7021 tgtctgcggc gtgagaactg cgacccacaa aaatcccaaa ccgcaatcgc acaaacaaat
     7081 agtgacacga aacagattat tctggtagct gttctcgcta tataagacaa tttttgagat
     7141 catatcatga tcaagacatc taaaggcatt cattttcgac tatattcttt tttacaaaaa
     7201 atataacaac cagatatttt aagctgatcc tagatgcaca aaaaataaat aaaagtataa
     7261 acctacttcg taggatactt cggggtactt tttgttcggg gttagatgag cataacgctt
     7321 gtagttgata tttgagatcc cctatcattg cagggtgaca gcggacgctt cgcagagctg
     7381 cattaaccag ggcttcgggc aggccaaaaa ctacggcacg ctccggccac ccagtccgcc
     7441 ggaggactcc ggttcaggga gcggccaact agccgagaac ctcacctatg cctggcacaa
     7501 tatggacatc tttggggcgg tcaatcagcc gggtcccgga tggcggcagc tggtcaaccg
     7561 gacacgcgga ctattctgca acgagcgaca cataccggcg cccaggaaac atttgctcaa
     7621 gaacggtgag tttctattcg cagtcggctg atctgtgtga aatcttaata aagggtccaa
     7681 ttaccaattt gaaactcagt ttgcggcgtg gcctatccgg gcgaactttt ggccgtgatg
     7741 ggcagttccg gtgccggaaa gacgaccctg ctgaatgccc ttgcctttcg atcgccgcag
     7801 ggcatccaag tatcgccatc cgggatgcga ctgctcaatg gccaacctgt ggacgccaag
     7861 gagatgcagg ccaggtgcgc ctatgtccag caggatgacc tctttatcgg ctccctaacg
     7921 gccagggaac acctgatttt ccaagccatg gtgcggatgc cacgacatct gacctatcgg
     7981 cagcgagtgg cccgcgtgga tcaggtgatc caggagcttt cgctcagcaa atgtcagcac
     8041 acgatcatcg gtgtgcccgg cagggtgaaa ggtctgtccg gcggagaaag gaagcgtctg
     8101 gcattcgcct ccgaggctct aaccgatccg ccgcttctga tctgcgatga gcccacctcc
     8161 ggactggact cctttaccgc ccacagcgtc gtccaggtgc tgaagaagct gtcgcagaag
     8221 ggcaagaccg tcatcctgac cattcatcag ccgtcttccg agctgtttga gctctttgac
     8281 aagatccttc tgatggccga gggcagggta gctttcttgg gcactcccag cgaagccgtc
     8341 gacttctttt cctagtgagt tcgatgtgtt tattaagggt atctagtatt acataacatc
     8401 tcaactccta tccagcgtgg gtgcccagtg tcctaccaac tacaatccgg cggactttta
     8461 cgtacaggtg ttggccgttg tgcccggacg ggagatcgag tcccgtgatc ggatcgccaa
     8521 gatatgcgac aattttgcca ttagcaaagt agcccgggat atggagcagt tgttggccac
     8581 caaaaatctg gagaagccac tggagcagcc ggagaatggg tacacctaca aggccacctg
     8641 gttcatgcag ttccgggcgg tcctgtggcg atcctggctg tcggtgctca aggaaccact
     8701 cctcgtaaaa gtgcgactta ttcagacaac ggtgagtggt tccagtggaa acaaatgata
     8761 taacgcttac aattcttgga aacaaattcg ctagatttta gatagaattg cctgattcca
     8821 cacccttctt agtttttttc aatgagatgt atagtttata gttttgcaga agataaataa
     8881 atttcattta actcgcgaat attaatgaga tgcgagtaac attttaattt gcagatggtt
     8941 gccatcttga ttggcctcat ctttttgggc caacaactca cgcaagtggg tgtgatgaat
     9001 atcaacggag ccatcttcct cttcctgacc aacatgacct ttcaaaacgt ctttgccacg
     9061 ataaatgtaa gtcatgttta gaatacattt gcatttcaat aatttactaa ctttctaatg
     9121 aatcgattcg atttaggtgt tcacctcaga gctgccagtt tttatgaggg aggcccgaag
     9181 tcgactttat cgctgtgaca catactttct gggcaaaacg attgccgaat tgccgctttt
     9241 tctcacagtg ccactggtct tcacggcgat tgcctatccg atgatcggac tgcgggccgg
     9301 agtgctgcac ttcttcaact gcctggcgct ggtcactctg gtggccaatg tgtcaacgtc
     9361 cttcggatat ctaatatcct gcgccagctc ctcgacctcg atggcgctgt ctgtgggtcc
     9421 gccggttatc ataccattcc tgctctttgg cggcttcttc ttgaactcgg gctcggtgcc
     9481 agtatacctc aaatggttgt cgtacctctc atggttccgt tacgccaacg agggtctgct
     9541 gattaaccaa tgggcggacg tggagccggg cgaaattagc tgcacatcgt cgaacaccac
     9601 gtgccccagt tcgggcaagg tcatcctgga gacgcttaac ttctccgccg ccgatctgcc
     9661 gctggactac gtgggtctgg ccattctcat cgtgagcttc cgggtgctcg catatctggc
     9721 tctaagactt cgggcccgac gcaaggagta gccgacatat atccgaaata actgcttgtt
     9781 tttttttttt accattatta ccatcgtgtt tactgtttat tgccccctca aaaagctaat
     9841 gtaattatat ttgtgccaat aaaaacaaga tatgacctat agaatacaag tatttcccct
     9901 tcgaacatcc ccacaagtag actttggatt tgtcttctaa ccaaaagact tacacacctg
     9961 cataccttac atcaaaaact cgtttatcgc tacataaaac accgggatat attttttata
    10021 tacatacttt tcaaatcgcg cgccctcttc ataattcacc tccaccacac cacgtttcgt
    10081 agttgctctt tcgctgtctc ccacccgctc tccgcaacac attcaccttt tgttcgacga
    10141 ccttggagcg actgtcgtta gttccgcgcg attcggttcg ctcaaatggt tccgagtggt
    10201 tcatttcgtc tcaatagaaa ttagtaataa atatttgtat gtacaattta tttgctccaa
    10261 tatatttgta tatatttccc tcacagctat atttattcta atttaatatt atgacttttt
    10321 aaggtaattt tttgtgacct gttcggagtg attagcgtta caatttgaac tgaaagtgac
    10381 atccagtgtt tgttccttgt gtagatgcat ctcaaaaaaa tggtgggcat aatagtgttg
    10441 tttatatata tcaaaaataa caactataat aataagaata catttaattt agaaaatgct
    10501 tggatttcac tggaactaga attaattcgg ctgctgctct aaacgacgca tttcgtactc
    10561 caaagtacga attttttccc tcaagctctt attttcatta aacaatgaac aggacctaac
    10621 gcacagtcac gttattgttt acataaatga ttttttttac tattcaaact tactctgttt
    10681 gtgtactccc actggtatag ccttctttta tcttttctgg ttcaggctct atcactttac
    10741 taggtacggc atctgcgttg agtcgcctcc ttttaaatgt ctgacctttt gcaggtgcag
    10801 ccttccactg cgaatcatta aagtgggtat cacaaatttg ggagttttca ccaaggctgc
    10861 acccaaggct ctgctcccac aattttctct taatagcaca cttcggcacg tgaattaatt
    10921 ttactccagt cacagctttg cagcaaaatt tgcaatattt catttttttt tattccacgt
    10981 aagggttaat gttttcaaaa aaaaattcgt ccgcacacaa cctttcctct caacaagcaa
    11041 acgtgcactg aatttaagtg tatacttcgg taagcttcgg ctatcgacgg gaccacctta
    11101 tgttatttca tcatgggcca gacccacgta gtccagcggc agatcggcgg cggagaagtt
    11161 aagcgtctcc aggatgacct tgcccgaact ggggcacgtg gtgttcgacg atgtgcagct
    11221 aatttcgccc ggctccacgt ccgcccattg gttaatcagc agaccctcgt tggcgtaacg
    11281 gaaccatgag aggtacgaca accatttgag gtatactggc accgagcccg agttcaagaa
    11341 gaaggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    11401 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    11461 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    11521 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    11581 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    11641 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    11701 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    11761 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
    11821 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    11881 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    11941 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    12001 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    12061 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    12121 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    12181 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    12241 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    12301 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    12361 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    12421 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    12481 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    12541 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    12601 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    12661 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    12721 gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    12781 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    12841 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    12901 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    12961 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    13021 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    13081 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
    13141 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
    13201 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
    13261 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
    13321 ggcatcagag cagattgtac tgagagtgca ccattcgaca tgcccgccgt gaccgtcgag
    13381 aacccgctga cgctgccccg cgtatccgca cccgccgacg ccgtcgcacg tcccgtgctc
    13441 accgtgacca ccgcgcccag cggtttcgag ggcgagggct tcccggtgcg ccgcgcgttc
    13501 gccgggatca actaccgcca cctcgacccg ttcatcatga tggaccagat gggtgaggtg
    13561 gagtacgcgc ccggggagcc caagggcacg ccctggcacc cgcaccgcgg cttcgagacc
    13621 gtgacctaca tcgtcgatat gcggtgtgaa ataccgcacc gaatcgcgcg gaactaacga
    13681 cagtcgctcc aaggtcgtcg aacaaaaggt gaatgtgttg cggagagcgg gtgggagaca
    13741 gcgaaagagc aactacgaaa cgtggtgtgg tggaggtgaa ttatgaagag ggcgcgcgat
    13801 ttgaaaagta tgtatataaa aaatatatcc cggtgtttta tgtagcgata aacgagtttt
    13861 tgatgtaagg tatgcaggtg tgtaagtctt ttggttagaa gacaaatcca aagtctactt
    13921 gtggggatgt tcgaagggga aatacttgta ttctataggt catatcttgt ttttattggc
    13981 acaaatataa ttacattagc tttttgaggg ggcaataaac agtaaacacg atggtaataa
    14041 tggtaaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
    14101 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
//
