LOCUS       Exported                9367 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pACU2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9367)
  AUTHORS   Han C, Jan LY, Jan YN
  TITLE     Enhancer-driven membrane markers for analysis of nonautonomous 
            mechanisms reveal neuron-glia interactions in Drosophila.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 May 23. ():.
  PUBMED    21606367
REFERENCE   2  (bases 1 to 9367)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9367
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(315..334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(994..1854)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1617..1636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1855..1959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2027..2045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2083..2105)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2205..2224
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    2434..2503
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    3046..3278
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    3340..3434
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        3453..3691
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     3638..3659
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             3702..3713
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     intron          3764..3896
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     3879..3898
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     complement(4644..4662)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(5122..5193,5588..5861,5936..6590,6652..6967,7188..7319,
                     7390..8004)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    8782..9367
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tcgacatgcc cgccgtgacc gtcgagaacc cgctgacgct
     2281 gccccgcgta tccgcacccg ccgacgccgt cgcacgtccc gtgctcaccg tgaccaccgc
     2341 gcccagcggt ttcgagggcg agggcttccc ggtgcgccgc gcgttcgccg ggatcaacta
     2401 ccgccacctc gacccgttca tcatgatgga ccagatgggt gaggtggagt acgcgcccgg
     2461 ggagcccaag ggcacgccct ggcacccgca ccgcggcttc gagaccgtga cctacatcgt
     2521 cgatatgcgg tgtgaaatac cgcaccgaat cgcgcggaac taacgacagt cgctccaagg
     2581 tcgtcgaaca aaaggtgaat gtgttgcgga gagcgggtgg gagacagcga aagagcaact
     2641 acgaaacgtg gtgtggtgga ggtgaattat gaagagggcg cgcgatttga aaagtatgta
     2701 tataaaaaat atatcccggt gttttatgta gcgataaacg agtttttgat gtaaggtatg
     2761 caggtgtgta agtcttttgg ttagaagaca aatccaaagt ctacttgtgg ggatgttcga
     2821 aggggaaata cttgtattct ataggtcata tcttgttttt attggcacaa atataattac
     2881 attagctttt tgagggggca ataaacagta aacacgatgg taataatggt aaaaaaaaaa
     2941 acaagcagtt atttcggata tatgtcggct actccttgcg tcgggcccga agtcttagag
     3001 ccagatatgc gagcacccgg aagctcacga tgagaatggc cagaccatga tgaaataaca
     3061 taaggtggtc ccgtcggcaa gagacatcca cttaacgtat gcttgcaata agtgcgagtg
     3121 aaaggaatag tattctgagt gtcgtattga gtctgagtga gacagcgata tgattgttga
     3181 ttaaccctta gcatgtccgt ggggtttgaa ttaactcata atattaatta gacgaaatta
     3241 tttttaaagt tttattttta ataatttgcg agtacgcaaa gcttggctgc atccaacgcg
     3301 ttgggagctc tccggatcca agcttgcatg cctgcaggtc ggagtactgt cctccgagcg
     3361 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag
     3421 tactgtcctc cgagcggaga ctctagcgag cgccggagta taaatagagg cgcttcgtct
     3481 acggagcgac aattcaattc aaacaagcaa agtgaacacg tcgctaagcg aaagctaagc
     3541 aaataaacaa gcgcagctga acaagctaaa caatctgcag taaagtgcaa gttaaagtga
     3601 atcaattaaa agtaaccagc aaccaagtaa atcaactgca actactgaaa tctgccaaga
     3661 agtaattatt gaatacaaga agagaactct gaatagggaa ttgggaattg cagtgactct
     3721 cttaaggtag ccttgcagaa gttggtcgtg aggcactggg caggtaagta tcaaggttac
     3781 aagacaggtt taaggagacc aatagaaact gggcttgtcg agacagagaa gactcttgcg
     3841 tttctgatag gcacctattg gtcttactga catccacttt gcctttctct ccacaggtgt
     3901 ccactcccag ttcaattaca gctcgaattc gttaacagat ctgcggccgc ggctcgaggg
     3961 tacctctaga ctaagccagt cggcaatcgg acgccttcga aacatgcaac actaatgttt
     4021 aattcagatt tcagcagaga caagctaaaa caccgacgag ttgtaatcat ttctgtgcgc
     4081 cagcatatat ttcttatata caacgtaata cataattatg taattctagc atctccccaa
     4141 cactcacata caaacaaaca aaaaatacaa acacacaaaa cgtatttacc cgcacgcatc
     4201 cttggcgagg ttgagtatga aacaaaaaca aaacttaatt tagagcaaag taattacacg
     4261 aataaattta ataaaaaaaa actataataa aaacgccaat catgttattt ccaaaaaaaa
     4321 aaaaaaaaaa aaaaactaga cgagatgttt gcgcttgtgt cgatgaatgt aaagaattgc
     4381 aactacatag atggtaatta atttcagctg taagaagtac aagtttttca taattgtgag
     4441 aaaagtgcag tagtatcgct taaagttcag ttttaatcag ggcatttagc agctcgaacc
     4501 caatgtattt gtaagcccaa taaacgtgta aatgcctaga gcaaaatatc acaactgtag
     4561 cttttcatcc gaaagataat aatgaataga ctgacctgac gtttttcaaa atgcgtagga
     4621 tccactagaa ggccttagta tgtatgtaag ttaataaaac ccttttttgg agaatgtaga
     4681 tttaaaaaaa catatttttt ttttattttt tactgcactg gacatcattg aacttatctg
     4741 atcagtttta aatttacttc gatccaaggg tatttgaagt accaggttct ttcgattacc
     4801 tctcactcaa aatgacattc cactcaaagt cagcgctgtt tgcctccttc tctgtccaca
     4861 gaaatatcgc cgtctctttc gccgctgcgt ccgctatctc tttcgccacc gtttgtagcg
     4921 ttacctagcg tcaatgtccg ccttcagttg cactttgtca gcggtttcgt gacgaagctc
     4981 caagcggttt acgccatcaa ttaaacacaa agtgctgtgc caaaactcct ctcgcttctt
     5041 atttttgttt gttttttgag tgattggggt ggtgattggt tttgggtggg taagcagggg
     5101 aaagtgtgaa aaatcccggc aatgggccaa gaggatcagg agctattaat tcgcggaggc
     5161 agcaaacacc catctgccga gcatctgaac aatgtgagta gtacatgtgc atacatctta
     5221 agttcacttg atctatagga actgcgattg caacatcaaa ttgtctgcgg cgtgagaact
     5281 gcgacccaca aaaatcccaa accgcaatcg cacaaacaaa tagtgacacg aaacagatta
     5341 ttctggtagc tgtgctcgct atataagaca atttttaaga tcatatcatg atcaagacat
     5401 ctaaaggcat tcattttcga ctacattctt ttttacaaaa aatataacaa ccagatattt
     5461 taagctgatc ctagatgcac aaaaaataaa taaaagtata aacctacttc gtaggatact
     5521 tcgttttgtt cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc
     5581 attgcagggt gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc
     5641 caaaaactac ggcacgctcc tgccacccag tccgccggag gactccggtt cagggagcgg
     5701 ccaactagcc gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa
     5761 tcagccgggc tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga
     5821 gcgacacata ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt
     5881 cggctgatct gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc
     5941 ggcgtggcct atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg
     6001 accctgctga atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg
     6061 atgcgactgc tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat
     6121 gtccagcagg atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccag
     6181 gccatggtgc ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag
     6241 gtgatccagg agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg
     6301 gtgaaaggtc tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggcactaacc
     6361 gatccgccgc ttctgatctg cgatgagccc acctccggac tggactcatt taccgcccac
     6421 agcgtcgtcc aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt
     6481 catcagccgt cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc
     6541 agggtagctt tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga
     6601 tgtgtttatt aagggtatct agcattacat tacatctcaa ctcctatcca gcgtgggtgc
     6661 ccagtgtcct accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc
     6721 cggacgggag atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgctattag
     6781 caaagtagcc cgggatatgg agcagttgtt ggccaccaaa aatttggaga agccactgga
     6841 gcagccggag aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct
     6901 gtggcgatcc tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca
     6961 gacaacggtg agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca
     7021 aattcgctag attttagtta gaattgcctg attccacacc cttcttagtt tttttcaatg
     7081 agatgtatag tttatagttt tgcagaaaat aaataaattt catttaactc gcgaacatgt
     7141 tgaagatatg aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct
     7201 tgattggcct catctttttg ggccaacaac tcacgcaagt gggcgtgatg aatatcaacg
     7261 gagccatctt cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg
     7321 taagtcttgt ttagaataca tttgcatatt aataatttac taactttcta atgaatcgat
     7381 tcgatttagg tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt
     7441 tatcgctgtg acacatactt tctgggcaaa acgattgccg aattaccgct ttttctcaca
     7501 gtgccactgg tcttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg
     7561 cacttcttca actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga
     7621 tatctaatat cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt
     7681 atcataccat tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac
     7741 ctcaaatggt tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac
     7801 caatgggcgg acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc
     7861 agttcgggca aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac
     7921 tacgtgggtc tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga
     7981 cttcgggccc gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt
     8041 ttaccattat taccatcgtg tttactgttt attgccccct caaaaagcta atgtaattat
     8101 atttgtgcca ataaaaacaa gatatgacct atagaataca agtatttccc cttcgaacat
     8161 ccccacaagt agactttgga tttgtcttct aaccaaaaga cttacacacc tgcatacctt
     8221 acatcaaaaa ctcgtttatc gctacataaa acaccgggat atatttttta tatacatact
     8281 tttcaaatcg cgcgccctct tcataattca cctccaccac accacgtttc gtagttgctc
     8341 tttcgctgtc tcccacccgc tctccgcaac acattcacct tttgttcgac gaccttggag
     8401 cgactgtcgt tagttccgcg cgattcggtt cgctcaaatg gttccgagtg gttcatttcg
     8461 tctcaataga aattagtaat aaatatttgt atgtacaatt tatttgctcc aatatatttg
     8521 tatatatttc cctcacagct atatttattc taatttaata ttatgacttt ttaaggtaat
     8581 tttttgtgac ctgttcggag tgattagcgt tacaatttga actgaaagtg acatccagtg
     8641 tttgttcctt gtgtagatgc atctcaaaaa aatggtgggc ataatagtgt tgtttatata
     8701 tatcaaaaat aacaactata ataataagaa tacatttaat ttagaaaatg cttggatttc
     8761 actggaacta gaattaattc ggctgctgct ctaaacgacg catttcgtac tccaaagtac
     8821 gaattttttc cctcaagctc ttattttcat taaacaatga acaggaccta acgcacagtc
     8881 acgttattgt ttacataaat gatttttttt actattcaaa cttactctgt ttgtgtactc
     8941 ccactggtat agccttcttt tatcttttct ggttcaggct ctatcacttt actaggtacg
     9001 gcatctgcgt tgagtcgcct ccttttaaat gtctgacctt ttgcaggtgc agccttccac
     9061 tgcgaatcat taaagtgggt atcacaaatt tgggagtttt caccaaggct gcacccaagg
     9121 ctctgctccc acaattttct cttaatagca cacttcggca cgtgaattaa ttttactcca
     9181 gtcacagctt tgcagcaaaa tttgcaatat ttcatttttt tttattccac gtaagggtta
     9241 atgttttcaa aaaaaaattc gtccgcacac aacctttcct ctcaacaagc aaacgtgcac
     9301 tgaatttaag tgtatacttc ggtaagcttc ggctatcgac gggaccacct tatgttattt
     9361 catcatg
//
