LOCUS       Exported                  20 bp ds-DNA     linear   SYN 02-JUN-2021
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human ROCK2.
ACCESSION   .
VERSION     .
KEYWORDS    ROCK2 gRNA (BRDN0001147800)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 20)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 2, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..20
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
ORIGIN
        1 tgtttaggga ggtacgactt
//
