LOCUS Exported 6941 bp ds-DNA linear SYN 29-MAY-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS pJT118 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6941) AUTHORS Tabor JJ, Levskaya A, Voigt CA TITLE Multichromatic control of gene expression in Escherichia coli. JOURNAL J Mol Biol. 2011 Jan 14. 405(2):315-24. PUBMED 21035461 REFERENCE 2 (bases 1 to 6941) AUTHORS . TITLE Direct Submission JOURNAL Exported May 29, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6941 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(2936..5980) /codon_start=1 /gene="lacZ" /product="beta-galactosidase" /label=lacZ /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ SGSLSQMWIGDKNQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD SWSPSVSAEFQLSAGRYHYQLVWCQK" primer_bind 5956..5978 /label=M13/pUC Forward /note="In lacZ gene" ORIGIN 1 ctaagctcga ggcaaatggt tatagcgttt taaacagata gtaactgttg ttccctggtc 61 aactgcactt tttagcaaga tactgccact gtgtaagtca acacatttct tggcaaccat 121 taaaccgagt cctgttcccg taatatttct gacatttttg ccccgatgaa agggttcaaa 181 aatttgcttt tggtcctctg gcgaaatgcc aatgccctgg tcggtgactt caaaaataat 241 ctgttccgaa tctaggctta gggcaatttt aatctgccct cccccgggag agtatttaat 301 cgcattagat aacagattag ataaaataga ccgcactaac ctttcatcca ccaatgcctt 361 cgtatcttga gcgctacaaa taaagtcaaa ataatattgg tcactgacac ttaattgaat 421 ttcttcgata aattgctgga acaataattt caaatctaac caattaggat taaattccaa 481 tttgcccgct tcggcacggt taatgattaa aatatcatcc aggagctgta ccatattttt 541 cacggaattt tgaatacggt gtaagttccg gctacgctta tcgggatcaa gccaggccac 601 ttcagaattt tccagtaatt gggcagcagc taaggccgta ctgaggggag tacgaaattc 661 atgggaagcc atggagaaaa aacgagtttt taggcggctt aattcttttt cccgttctaa 721 agctaggcgg acttcctccg tgcgtcggcg ctcagtgaca tccctttgca cagcaatcca 781 atgggtgtaa aaacctgttt tattggcaac gggcaccaga ctaaattcca cccaaaactc 841 actgccatcc ttacgataat taatcacttc aacggtaact gattgccatt gactaatggc 901 ttgccgcacc ctatctaatt cagtgcgact agtttttggt ccctgtaaaa ctcggggggt 961 tttgcctagc atttcttcag cagtataacc ggtgatttta gtaaatgctt cattgacata 1021 gagaattctc ggcccgggat catcaatggg ctccgcttcc gtaatcacca ccgcatcatt 1081 agtattaacc acaacggatt gtagcaaacg aatttgttct gcctgccgct tttgctcggt 1141 aatatcggcg acaaccgcca ccaatccggc aatattttct tcactatcca ataggggagc 1201 agcggaaagg acaatttcaa tccaactacc atcttttttt tgacatttta attctaggct 1261 aggggaaacc atgcctgata aaactttctg tttaaattgc tgataatctt ccagcaaaat 1321 gttggatgtt aataattctg gatgggcaat aatttctgtt tctgtccaac caaaaatacg 1381 ttctgctgta ggattccaaa tctgaatttg gtctgctaaa ttaagggtaa aaatacccct 1441 gggggaggcc gcaatcaggg attgcagagt atggttagtg tggcggagag cggcttccgt 1501 tttttgtcgc tcactgattt ccattcttag ggattgattg gtggcggcaa gttgctgggt 1561 gcgtttttcg actcggtttt ccaaatcttt attgagttgc tgtaattgct catataattc 1621 cgattgttgg atggcgatcg cgacctgatt ggctagctgt ttcattaact ccacttccca 1681 cggttgccat ggccgggtaa aagcacattg atgggtaatt aacagccccc aaagataggg 1741 aaattctgat tcattatcta gggaagaagc acgattatgt tgaagaatgg gcactactaa 1801 ttttgatttc acgccaaatt gtttgacgaa atcagctagg caaatctcta tgtcatcctg 1861 gtcaatgtca ttaatggccc gtactttacc tttggtgtag gcttgatggt attcaacggg 1921 aaaaacttca tcggaaaagg tccgccctaa aatactagga taattggcat tcaccgattc 1981 cgtaatggcg ctgcccgtgc catcttgcca aatgcgataa attagaactc gatcaacttg 2041 caacagggtt ttaacttcag cgacggtggt atttaaaacc gtttccaagt ttaaagattg 2101 gcggatatgt tgggtaattt ggttaataaa tcgttcccgt tgggtttgtt gcttgatgct 2161 aatttcaatc ctgcggcgtt cttggttttg tctgtgccat aaataacagg tcatggcgat 2221 cgccagaaaa acaaattcga ttggaattag aaatttgccc atatattagc gaatcccagt 2281 agaaaaacct tacttaaaat atttttgttt ggcaataatt ttcatgactt ttaacaataa 2341 agctaaatca tgatccttgg caaaaacgcc gtcaatttgg ggcaggtaac tgcgagcttg 2401 taccgtgatc tgactgtcgg cacaatccgt aattagtagt agggccggcg gcttagcaga 2461 caatttttta ataatggtca ctaaatctaa agtagctgaa atatcaattg attcatattt 2521 aatcccgcaa ccaactagga tcgaatgata ttctccaacg tcaacattgt taaacaaaat 2581 atcttggaag ttgatagcag aaatttcaaa acttggacat aaaaatcgct tgactgattg 2641 ataccactgc cgatccattt ctaggatacc aatttttgcc ttcgttttct tgcaggtcag 2701 ggcagaggct tttttaccgg ctagtttcaa cattggagtc tggcctcaaa tacaaagtct 2761 gcaaagtctc gaaatattaa accagagaat aaataattaa acgccaaaat atttctagca 2821 aaaaccccag ttattaaacc gcctaagtcc cccaggaaag ggggatataa cagtatagat 2881 tttgtcagcc ttcagcttgg ctttaccgtc aaaaaaatta gactcgagcg gccgcttttt 2941 gacaccagac caactggtaa tggtagcgac cggcgctcag ctggaattcc gccgatactg 3001 acgggctcca ggagtcgtcg ccaccaatcc ccatatggaa accgtcgata ttcagccatg 3061 tgccttcttc cgcgtgcagc agatggcgat ggctggtttc catcagttgc tgttgactgt 3121 agcggctgat gttgaactgg aagtcgccgc gccactggtg tgggccataa ttcaattcgc 3181 gcgtcccgca gcgcagaccg ttttcgctcg ggaagacgta cggggtatac atgtctgaca 3241 atggcagatc ccagcggtca aaacaggcgg cagtaaggcg gtcgggatag ttttcttgtg 3301 gccctaatcc gagccagttt acccgctctg ctacctgcgc cagctggcag ttcaggccaa 3361 tccgcgccgg atgcggtgta tcgctcgcca cttcaacatc aacggtaatc gccatttgac 3421 cactaccatc aatccggtag gttttccggc tgataaataa ggttttcccc tgatgctgcc 3481 acgcgtgagc ggtcgtaatc agcaccgcat cagcaagtgt atctgccgtg cactgcaaca 3541 acgctgcttc ggcctggtaa tggcccgccg ccttccagcg ttcgacccag gcgttagggt 3601 caatgcgggt cgcttcactt acgccaatgt cgttatccag cggtgcacgg gtgaactgat 3661 cgcgcagcgg cgtcagcagt tggtttttat cgccaatcca catctgtgaa agagagcctg 3721 actggcggtt aaattgccaa cgcttattac ccagctcgat gcaaaaatcc atttcgctgg 3781 tggtcagatg cgggatggcg tgggacgcgg cggggagcgt cacactgagg ttttccgcca 3841 gacgccactg ctgccaggcg ctgatgtgcc cggcttctga ccatgcggtc gcgttcggtt 3901 gcactacgcg tactgtgagc cagagttgcc cggcgctctc cggctgcggt agttcaggca 3961 gttcaatcaa ctgtttacct tgtggagcga catccagagg cacttcaccg cttgccagcg 4021 gcttaccatc cagcgccacc atccagtgca ggagctcgtt atcgctatga cggaacaggt 4081 attcgctggt cacttcgatg gtttgcccgg ataaacggaa ctggaaaaac tgctgctggt 4141 gttttgcttc cgtcagcgct ggatgcggcg tgcggtcggc aaagaccaga ccgttcatac 4201 agaactggcg atcgttcggc gtatcgccaa aatcaccgcc gtaagccgac cacgggttgc 4261 cgttttcatc atatttaatc agcgactgat ccacccagtc ccagacgaag ccgccctgta 4321 aacggggata ctgacgaaac gcctgccagt atttagcgaa accgccaaga ctgttaccca 4381 tcgcgtgggc gtattcgcaa aggatcagcg ggcgcgtctc tccaggtagc gaaagccatt 4441 ttttgatgga ccatttcggc acagccggga agggctggtc ttcatccacg cgcgcgtaca 4501 tcgggcaaat aatatcggtg gccgtggtgt cggctccgcc gccttcatac tgcaccgggc 4561 gggaaggatc gacagatttg atccagcgat acagcgcgtc gtgattagcg ccgtggcctg 4621 attcattccc cagcgaccag atgatcacac tcgggtgatt acgatcgcgc tgcaccattc 4681 gcgttacgcg ttcgctcatc gccggtagcc agcgcggatc atcggtcaga cgattcattg 4741 gcaccatgcc gtgggtttca atattggctt catccaccac atacaggccg tagcggtcgc 4801 acagcgtgta ccacagcgga tggttcggat aatgcgaaca gcgcacggcg ttaaagttgt 4861 tctgcttcat cagcaggata tcctgcacca tcgtctgctc atccatgacc tgaccatgca 4921 gaggatgatg ctcgtgacgg ttaacgcctc gaatcagcaa cggcttgccg ttcagcagca 4981 gcagaccatt ttcaatccgc acctcgcgga aaccgacatc gcaggcttct gcttcaatca 5041 gcgtgccgtc ggcggtgtgc agttcaacca ccgcacgata gagattcggg atttcggcgc 5101 tccacagttt cgggttttcg acgttcagac gtagtgtgac gcgatcggca taaccaccac 5161 gctcatcgat aatttcaccg ccgaaaggcg cggtgccgct ggcgacctgc gtttcaccct 5221 gccataaaga aactgttacc cgtaggtagt cacgcaactc gccgcacatc tgaacttcag 5281 cctccagtac agcgcggctg aaatcatcat taaagcgagt ggcaacatgg aaatcgctga 5341 tttgtgtagt cggtttatgc agcaacgaga cgtcacggaa aatgccgctc atccgccaca 5401 tatcctgatc ttccagataa ctgccgtcac tccagcgcag caccatcacc gcgaggcggt 5461 tttctccggc gcgtaaaaat gcgctcaggt caaattcaga cggcaaacga ctgtcctggc 5521 cgtaaccgac ccagcgcccg ttgcaccaca gatgaaacgc cgagttaacg ccatcaaaaa 5581 taattcgcgt ctggccttcc tgtagccagc tttcatcaac attaaatgtg agcgagtaac 5641 aacccgtcgg attctccgtg ggaacaaacg gcggattgac cgtaatggga taggtcacgt 5701 tggtgtagat gggcgcatcg taaccgtgca tctgccagtt tgaggggacg acgacagtat 5761 cggcctcagg aagatcgcac tccagccagc tttccggcac cgcttctggt gccggaaacc 5821 aggcaaagcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg 5881 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg 5941 taacgccagg gttttcccag tcacgacgtt gtaaaacgac gggatctatc atagataaag 6001 ttagtaatta aacttaaaag ttgtttaatg tccagccaat aactggtatg tgttggcatt 6061 aaaaatgcga tcctaacaaa gtaaaattga agaaaagggg gagaaaatcg taaagaaatc 6121 ggaaagttag ctgatgcgac taaaactgtt acatttcgca acatgaatta acccagacct 6181 gttcacccct tcaggtaagc tgaggttgat agagaaaagc acaatgggct atgagaattc 6241 ttttagtgga ggatgatttg ccgctggcgg aaacccttgc tgaagcattg agtgaccagc 6301 tttacaccgt tgatattgcc accgacgctt ccctcgcctg ggactatgcc tcccgactgg 6361 aatatgacct cgttattttg gatgtgatgc tgccggagtt ggacgggatt accctctgtc 6421 aaaaatggcg atcgcacagt tatttaatgc caattttgat gatgacagcc agggatacga 6481 tcaatgataa aatcacgggc ttggatgcgg gggcggatga ttatgtggtc aagccagtgg 6541 atttggggga gttatttgcc agggtgcgag ctttgttgcg tcggggttgt gcaacgtgcc 6601 aaccagtttt agagtggggg ccaatcaggt tggatccaag cacctatgaa gttagttatg 6661 acaatgaggt tttgtctttg acccgcaagg aatacagcat tctggaatta ctactccgca 6721 atggccgtcg ggtgctaagt cggagcatga ttatcgatag tatctggaag ttggagagtc 6781 ccccagagga agatacggtt aaggtgcatg tgcggagttt gcgacaaaaa ttaaaaagtg 6841 ccggtttatc agcagatgcc attgaaacgg tccatggcat tgggtatcgt ctggccaatt 6901 taacggaaaa atctttgtgc caagggaaaa actagtctag a //