LOCUS Exported 13805 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Plant expression of Cas9 and gRNA empty backbone; Basta resistance. ACCESSION . VERSION . KEYWORDS pBAtC SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 13805) AUTHORS Kim H, Kim ST, Ryu J, Choi MK, Kweon J, Kang BC, Ahn HM, Bae S, Kim JS, Kim SG TITLE A simple, flexible and high-throughput cloning system for plant genome editing via CRISPR-Cas system. JOURNAL J Integr Plant Biol. 2016 Mar 4. doi: 10.1111/jipb.12474. PUBMED 26946469 REFERENCE 2 (bases 1 to 13805) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..13805 /organism="synthetic DNA construct" /mol_type="other DNA" misc_RNA complement(13..88) /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" promoter 1005..1349 /label=CaMV 35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" primer_bind 1293..1313 /label=35S promoter /note="CaMV 35S promoter, forward primer" protein_bind 1449..1473 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 1476..5579 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 5595..5615 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 5616..5642 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" protein_bind complement(5647..5671) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" misc_feature 5991..6015 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" CDS 7346..7975 /codon_start=1 /product="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 StaA /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" CDS 8404..9477 /codon_start=1 /product="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 RepA /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI LVMRYRNLIEGEASAGS" rep_origin 9543..9737 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" primer_bind complement(9973..9995) /label=pGEX 3' /note="pGEX vectors, reverse primer" misc_feature 10081..10221 /label=bom /note="basis of mobility region from pBR322" primer_bind 10136..10155 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(10236..10253) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(10407..10995) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(10487..10506) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(11241..12032) /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" misc_feature 12553..12577 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" terminator 12670..12922 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" CDS complement(12942..13493) /codon_start=1 /gene="Streptomyces hygroscopicus bar" /product="phosphinothricin acetyltransferase" /label=BlpR /note="confers resistance to bialophos or phosphinothricin" /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF WQLDFSLPVPPRPVLPVTEI" promoter complement(13535..13714) /label=NOS promoter /note="nopaline synthase promoter" ORIGIN 1 gagctcaaaa aagcaccgac tcggtgccac tttttcaagt tgataacgga ctagccttat 61 tttaacttgc tatttctagc tctaaaacct ctgcaggtga ctctcgagac acctgcctcc 121 aatcactact tcgactctag ctgtatataa actcagcttc gttttcttat ctaagcgatg 181 tgggactttt gaagattgtt ttcaacttaa atgggcctat ataagaaata ctattgttct 241 ttcccatata aatgggcctg cttctcttct ttcagattcc caggggcctt ttgaagatta 301 tcttcatatc ttaagaatga agatgtttta ttcaatcaaa ttcttgaagg ttcgatgcct 361 aatcattcga attcgagctc tcccatatgg tcgactagag ccaagctgat ctcctttgcc 421 ccggagatca ccatggacga ctttctctat ctctacgatc taggaagaaa gttcgacgga 481 gaaggtgacg ataccatgtt caccaccgat aatgagaaga ttagcctctt caatttcaga 541 aagaatgctg acccacagat ggttagagag gcctacgcgg caggtctcat caagacgatc 601 tacccgagta ataatctcca ggagatcaaa taccttccca agaaggttaa agatgcagtc 661 aaaagattca ggactaactg catcaagaac acagagaaag atatatttct caagatcaga 721 agtactattc cagtatggac gattcaaggc ttgcttcata aaccaaggca agtaatagag 781 attggagtct ctaagaaagt agttcctact gaatcaaagg ccatggagtc aaaaattcag 841 atcgaggatc taacagaact cgccgtgaag actggcgaac agttcataca gagtctttta 901 cgactcaatg acaagaagaa aatcttcgtc aacatggtgg agcacgacac tctcgtctac 961 tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa 1021 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa 1081 aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct 1141 atcgttcaag atgcccctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 1201 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc 1261 tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata 1321 taaggaagtt catttcattt ggagaggact ccggtatttt tacaacaatt accacaacaa 1381 aacaaacaac aaacaacatt acaatttact attctagtcg acctgcaggc ggccgcacta 1441 gtgatatcac aagtttgtac aaaaaagcag gcttcatgga caagaagtac agcatcggcc 1501 tggacatcgg taccaacagc gtgggctggg ccgtgatcac cgacgagtac aaggtgccca 1561 gcaagaagtt caaggtgctg ggcaacaccg accgccacag catcaagaag aacctgatcg 1621 gcgccctgct gttcgacagc ggcgagaccg ccgaggccac ccgcctgaag cgcaccgccc 1681 gccgccgcta cacccgccgc aagaaccgca tctgctacct gcaggagatc ttcagcaacg 1741 agatggccaa ggtggacgac agcttcttcc accgcctgga ggagagcttc ctggtggagg 1801 aggacaagaa gcacgagcgc caccccatct tcggcaacat cgtggacgag gtggcctacc 1861 acgagaagta ccccaccatc taccatctgc gcaagaagct ggtggacagc accgacaagg 1921 ccgacctgcg cctgatctac ctggccctgg cccacatgat caagttccgc ggccacttcc 1981 tgatcgaggg cgacctgaac cccgacaaca gcgacgtgga caagctgttc atccagctgg 2041 tgcagaccta caaccagctg ttcgaggaga accccatcaa cgccagcggc gtggacgcca 2101 aggccatcct gagcgcccgc ctgagcaaga gccgccgcct ggagaacctg atcgcccagc 2161 tgcccggcga gaagaagaac ggcctgttcg gcaacctgat cgccctgagc ctgggcctga 2221 cccccaactt caagagcaac ttcgacctgg ccgaggacgc caagctgcag ctgagcaagg 2281 acacctacga cgacgacctg gacaacctgc tggcccagat cggcgaccag tacgccgacc 2341 tgttcctggc cgccaagaac ctgagcgacg ccatcctgct gagcgacatc ctgcgcgtga 2401 acaccgagat caccaaggcc cccctgagcg ccagcatgat caagcgctac gacgagcacc 2461 accaggacct gaccctgctg aaggccctgg tgcgccagca gctgcccgag aagtacaagg 2521 agatcttctt cgaccagagc aagaacggct acgccggcta catcgacggc ggcgccagcc 2581 aggaggagtt ctacaagttc atcaagccca tcctggagaa gatggacggc accgaggagc 2641 tgctggtgaa gctgaaccgc gaggacctgc tgcgcaagca gcgcaccttc gacaacggca 2701 gcatccccca ccagatccac ctgggcgagc tgcacgccat cctgcgccgc caggaggact 2761 tctacccctt cctgaaggac aaccgcgaga agatcgagaa gatcctgacc ttccgcatcc 2821 cctactacgt gggccccctg gcccgcggca acagccgctt cgcctggatg acccgcaaga 2881 gcgaggagac catcaccccc tggaacttcg aggaggtggt ggacaagggc gccagcgccc 2941 agagcttcat cgagcgcatg accaacttcg acaagaacct gcccaacgag aaggtgctgc 3001 ccaagcacag cctgctgtac gagtacttca ccgtgtacaa cgagctgacc aaggtgaagt 3061 acgtgaccga gggcatgcgc aagcccgcct tcctgagcgg cgagcagaag aaggccatcg 3121 tggacctgct gttcaagacc aaccgcaagg tgaccgtgaa gcagctgaag gaggactact 3181 tcaagaagat cgagtgcttc gacagcgtgg agatcagcgg cgtggaggac cgcttcaacg 3241 ccagcctggg cacctaccac gacctgctga agatcatcaa ggacaaggac ttcctggaca 3301 acgaggagaa cgaggacatc ctggaggaca tcgtgctgac cctgaccctg ttcgaggacc 3361 gcgagatgat cgaggagcgc ctgaagacct acgcccacct gttcgacgac aaggtgatga 3421 agcagctgaa gcgccgccgc tacaccggct ggggccgcct gagccgcaag cttatcaacg 3481 gcatccgcga caagcagagc ggcaagacca tcctggactt cctgaagagc gacggcttcg 3541 ccaaccgcaa cttcatgcag ctgatccacg acgacagcct gaccttcaag gaggacatcc 3601 agaaggccca ggtgagcggc cagggcgaca gcctgcacga gcacatcgcc aacctggccg 3661 gcagccccgc catcaagaag ggcatcctgc agaccgtgaa ggtggtggac gagctggtga 3721 aggtgatggg ccgccacaag cccgagaaca tcgtgatcga gatggcccgc gagaaccaga 3781 ccacccagaa gggccagaag aacagccgcg agcgcatgaa gcgcatcgag gagggcatca 3841 aggagctggg cagccagatc ctgaaggagc accccgtgga gaacacccag ctgcagaacg 3901 agaagctgta cctgtactac ctgcagaacg gccgcgacat gtacgtggac caggagctgg 3961 acatcaaccg cctgagcgac tacgacgtgg accacatcgt gccccagagc ttcctgaagg 4021 acgacagcat cgacaacaag gtgctgaccc gcagcgacaa gaaccgcggc aagagcgaca 4081 acgtgcccag cgaggaggtg gtgaagaaga tgaagaacta ctggcgccag ctgctgaacg 4141 ccaagctgat cacccagcgc aagttcgaca acctgaccaa ggccgagcgc ggcggcctga 4201 gcgagctgga caaggccggc ttcatcaagc gccagctggt ggagacccgc cagatcacca 4261 agcacgtggc ccagatcctg gacagccgca tgaacaccaa gtacgacgag aacgacaagc 4321 tgatccgcga ggtgaaggtg atcaccctga agagcaagct ggtgagcgac ttccgcaagg 4381 acttccagtt ctacaaggtg cgcgagatca acaactacca ccacgcccac gacgcctacc 4441 tgaacgccgt ggtgggcacc gccctgatca agaagtaccc caagctggag agcgagttcg 4501 tgtacggcga ctacaaggtg tacgacgtgc gcaagatgat cgccaagagc gagcaggaga 4561 tcggcaaggc caccgccaag tacttcttct acagcaacat catgaacttc ttcaagaccg 4621 agatcaccct ggccaacggc gagatccgca agcgccccct gatcgagacc aacggcgaga 4681 ccggcgagat cgtgtgggac aagggccgcg acttcgccac cgtgcgcaag gtgctgagca 4741 tgccccaggt gaacatcgtg aagaagaccg aggtgcagac cggcggcttc agcaaggaga 4801 gcatcctgcc caagcgcaac agcgacaagc tgatcgcccg caagaaggac tgggacccca 4861 agaagtacgg cggcttcgac agccccaccg tggcctacag cgtgctggtg gtggccaagg 4921 tggagaaggg caagagcaag aagctgaaga gcgtgaagga gctgctgggc atcaccatca 4981 tggagcgcag cagcttcgag aagaacccca tcgacttcct ggaggccaag ggctacaagg 5041 aggtgaagaa ggacctgatc atcaagctgc ccaagtacag cctgttcgag ctggagaacg 5101 gccgcaagcg catgctggcc agcgccggcg agctgcagaa gggcaacgag ctggccctgc 5161 ccagcaagta cgtgaacttc ctgtacctgg ccagccacta cgagaagctg aagggcagcc 5221 ccgaggacaa cgagcagaag cagctgttcg tggagcagca caagcactac ctggacgaga 5281 tcatcgagca gatcagcgag ttcagcaagc gcgtgatcct ggccgacgcc aacctggaca 5341 aggtgctgag cgcctacaac aagcaccgcg acaagcccat ccgcgagcag gccgagaaca 5401 tcatccacct gttcaccctg accaacctgg gcgcccccgc cgccttcaag tacttcgaca 5461 ccaccatcga ccgcaagcgc tacaccagca ccaaggaggt gctggacgcc accctgatcc 5521 accagagcat caccggtctg tacgagaccc gcatcgacct gagccagctg ggcggcgacg 5581 gcggctccgg acctccaaag aaaaagagaa aagtataccc ctacgacgtg cccgactacg 5641 cctaacaccc agctttcttg tacaaagtgg tgatatcccg cggccatgct agagtccgca 5701 aaaatcacca gtctctctct acaaatctat ctctctctat ttttctccag aataatgtgt 5761 gagtagttcc cagataaggg aattagggtt cttatagggt ttcgctcatg tgttgagcat 5821 ataagaaacc cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa 5881 ttcctaaaac caaaatccag tgacctgcag gcatgcgacg tcgggcccaa gcttagcttg 5941 agcttggatc agattgtcgt ttcccgcctt cagtttaaac tatcagtgtt tgacaggata 6001 tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat atttaaaagg 6061 gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat gccaaccaca gggttcccct 6121 cgggatcaaa gtactttaaa gtactttaaa gtactttaaa gtactttgat ccaacccctc 6181 cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga aaacgacatg 6241 tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg gcgttttctt 6301 gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag acattacgcc 6361 atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac cgacgaccag 6421 gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct gttttccgag 6481 aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga ccacctacgc 6541 cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta 6601 ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg 6661 tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc cggcattgcc 6721 gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc cgccaaggcc 6781 cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc 6841 gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat 6901 cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac cgaggccagg 6961 cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc ggccgccgag 7021 aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac gaaccgtttt 7081 tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc gagccgcccg 7141 cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg 7201 cggcctggcc ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat 7261 gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa 7321 ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg 7381 gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg ccggggccga 7441 tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg ccgtgcggga 7501 agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc 7561 catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg acttggctgt 7621 gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc cttacgacat 7681 atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca cggatggaag 7741 gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt 7801 tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca cgcagcgcgt 7861 gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac ccgagggcga 7921 cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca tttgagttaa 7981 tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg tccgagcgca 8041 cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat gaagcgggtc 8101 aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt acgccaaggc 8161 aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta aatgagcaaa 8221 tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa atcaagaaca 8281 accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt ggccaggcgt 8341 aagcggctgg gttgtctgcc ggccctgcaa tggcactgga acccccaagc ccgaggaatc 8401 ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac 8461 ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca 8521 cgccccggtg aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg 8581 ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca accagatttt 8641 ttcgttccga tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc 8701 gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca 8761 gacgggcacg tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg ggattacgac 8821 ctggtactga tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag 8881 ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg 8941 cgagccgatg gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc 9001 acgcacgttg ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc 9061 gagggtgaag ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag 9121 tacatcgaga tcgagctagc tgattggatg taccgcgaga tcacagaagg caagaacccg 9181 gacgtgctga cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc 9241 taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc 9301 tacgaacgca gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg 9361 atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg 9421 atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt 9481 acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa aggtctcttt 9541 cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac 9601 attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa 9661 gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc 9721 cgcctggcct gtgcataact gtctggccag cgcacagccg aagagctgca aaaagcgcct 9781 acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct 9841 ggccgctcaa aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg 9901 ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga 9961 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc 10021 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg 10081 cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca 10141 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta 10201 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 10261 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 10321 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 10381 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 10441 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 10501 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 10561 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 10621 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 10681 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 10741 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 10801 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt 10861 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 10921 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 10981 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 11041 gaaaactcac gttaagggat tttggtcatg catgatatat ctcccaattt gtgtagggct 11101 tattatgcac gcttaaaaat aataaaagca gacttgacct gatagtttgg ctgtgagcaa 11161 ttatgtgctt agtgcatcta acgcttgagt taagccgcgc cgcgaagcgg cgtcggcttg 11221 aacgaatttc tagctagaca ttatttgccg actaccttgg tgatctcgcc tttcacgtag 11281 tggacaaatt cttccaactg atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga 11341 taagcctgtc tagcttcaag tatgacgggc tgatactggg ccggcaggcg ctccattgcc 11401 cagtcggcag cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg 11461 gacaacgtaa gcactacatt tcgctcatcg ccagcccagt cgggcggcga gttccatagc 11521 gttaaggttt catttagcgc ctcaaataga tcctgttcag gaaccggatc aaagagttcc 11581 tccgccgctg gacctaccaa ggcaacgcta tgttctcttg cttttgtcag caagatagcc 11641 agatcaatgt cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt gcgctgccat 11701 tctccaaatt gcagttcgcg cttagctgga taacgccacg gaatgatgtc gtcgtgcaca 11761 acaatggtga cttctacagc gcggagaatc tcgctctctc caggggaagc cgaagtttcc 11821 aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc 11881 agcaaatcaa tatcactgtg tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt 11941 acggccagca acgtcggttc gagatggcgc tcgatgacgc caactacctc tgatagttga 12001 gtcgatactt cggcgatcac cgcttccccc atgatgttta actttgtttt agggcgactg 12061 ccctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc 12121 gcttgctgct tggatgcccg aggcatagac tgtaccccaa aaaaacagtc ataacaagcc 12181 atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg 12241 cgtgagcgca tacgctactt gcattacagc ttacgaaccg aacaggctta tgtccactgg 12301 gttcgtgccc gaattgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta 12361 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag 12421 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg 12481 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg 12541 ttggctggct ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa 12601 taacacattg cggacgtttt taatgtactg aattaacgcc gaattgaatt atcagcttgc 12661 atgccggtcg atctagtaac atagatgaca ccgcgcgcga taatttatcc tagtttgcgc 12721 gctatatttt gttttctatc gcgtattaaa tgtataattg cgggactcta atcataaaaa 12781 cccatctcat aaataacgtc atgcattaca tgttaattat tacatgctta acgtaattca 12841 acagaaatta tatgataatc atcgcaagac cggcaacagg attcaatctt aagaaacttt 12901 attgccaaat gtttgaacga tctgcttgac tctaggggtc atcagatttc ggtgacgggc 12961 aggaccggac ggggcggcac cggcaggctg aagtccagct gccagaaacc cacgtcatgc 13021 cagttcccgt gcttgaagcc ggccgcccgc agcatgccgc ggggggcata tccgagcgcc 13081 tcgtgcatgc gcacgctcgg gtcgttgggc agcccgatga cagcgaccac gctcttgaag 13141 ccctgtgcct ccagggactt cagaaggtgg gtgtagagcg tggagcccag tcccgtccgc 13201 tggtggcggg gggagacgta cacggtcgac tcggccgtcc agtcgtaggc gttgcgtgcc 13261 ttccagggac ccgcgtaggc gatgccggcg acctcgccgt ccacctcggc gacgagccag 13321 ggatagcgct cccgcagacg gacgaggtcg tccgtccact cctgcggttc ctgcggctcg 13381 gtacggaagt tgaccgtgct tgtctcgatg tagtggttga cgatggtgca gaccgccggc 13441 atgtccgcct cggtggcacg gcggatgtcg gccgggcgtc gttctgggct catggtagat 13501 cccctcgatc gagttgagag tgaatatgag actctaattg gataccgagg ggaatttatg 13561 gaacgtcagt ggagcatttt tgacaagaaa tatttgctag ctgatagtga ccttaggcga 13621 cttttgaacg cgcaataatg gtttctgacg tatgtgctta gctcattaaa ctccagaaac 13681 ccgcggctca gtggctcctt caacgttgcg gttctgtcag ttccaaacgt aaaacggctt 13741 gtcccgcgtc atcggcgggg gtcataacgt gactccctta attctccgct catgatcgat 13801 aattc //