LOCUS       Exported               11752 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses tdPP7-3xmCherry.
ACCESSION   .
VERSION     .
KEYWORDS    pHR-tdPP7-3xmCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11752)
  AUTHORS   Yan X, Hoek TA, Vale RD, Tanenbaum ME
  TITLE     Dynamics of Translation of Single mRNA Molecules In Vivo.
  JOURNAL   Cell. 2016 May 5;165(4):976-89. doi: 10.1016/j.cell.2016.04.034.
  PUBMED    27153498
REFERENCE   2  (bases 1 to 11752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11752
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(302..321)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     655..677
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(715..733)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        801..905
                     /gene="bla"
                     /label=AmpR promoter
     CDS             906..1766
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1124..1143)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1937..2525
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2426..2445
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2679..2696
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2766..2786)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2771..3100
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2951..3086
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3013..3032
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     intron          4234..4299
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4429..4449
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4874..5008
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4911..4930)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4965..4984
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5177..5810
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5857..5982
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6474..6707
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6892..6936
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7221..7338
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7491..7898
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             8797..9501
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     CDS             9547..9708
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     CDS             9733..10437
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     CDS             10483..10644
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     CDS             10702..11406
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     LTR             11488..11721
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(11746..12)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(11747..12)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tgtcacctaa atcgtatgtg tatgatacat aaggttatgt attaattgta gccgcgttct
       61 aacgacaata tgtacaagcc taattgtgta gcatctggct tactgaagca gaccctatca
      121 tctctctcgt aaactgccgt cagagtcggt ttggttggac gaaccttctg agtttctggt
      181 aacgccgtcc cgcacccgga aatggtcagc gaaccaatca gcagggtcat cgctagccag
      241 atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc
      301 gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc
      361 gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc
      421 tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc
      481 tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc tcagtacaat
      541 ctagctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
      601 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
      661 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
      721 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
      781 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
      841 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      901 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      961 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     1021 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     1081 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     1141 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     1201 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     1261 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     1321 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     1381 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     1441 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     1501 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     1561 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     1621 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     1681 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     1741 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1801 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1861 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1921 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1981 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     2041 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     2101 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     2161 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     2221 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     2281 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     2341 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     2401 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     2461 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     2521 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     2581 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     2641 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     2701 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     2761 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     2821 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     2881 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     2941 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     3001 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     3061 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttggac acaagacagg
     3121 cttgcgagat atgtttgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa
     3181 aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc
     3241 aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca
     3301 tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa
     3361 gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgt
     3421 accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc
     3481 cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg
     3541 cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgcgg ttgcgattcg
     3601 tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct
     3661 ggttgatgac tatgttgttg atatcccgca agatacctgg attgaacagc cgtgggatat
     3721 gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc
     3781 gggcgttgtt ctttttaact tcaggcgggt tacaatagtt tccagtaagt attctggagg
     3841 ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc
     3901 ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg
     3961 cccttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct
     4021 ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc acgtattgtg atgagcgatg
     4081 ccgaacgtac cgacgatgat ttatacgata cggtgattgg ctaccgtggc ggcaactgga
     4141 tttatgagtg ggccccggat ctttgtgaag gaaccttact tctgtggtgt gacataattg
     4201 gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa
     4261 tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat
     4321 gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg
     4381 ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag
     4441 agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct
     4501 gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca
     4561 ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt
     4621 tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat
     4681 aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa
     4741 tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt
     4801 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     4861 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     4921 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     4981 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc
     5041 caaacttccc accccatacc ctattaccac tgccaattac ctagtggttt catttactct
     5101 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
     5161 ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat
     5221 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca
     5281 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
     5341 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
     5401 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
     5461 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc
     5521 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
     5581 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg
     5641 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     5701 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     5761 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     5821 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     5881 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     5941 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     6001 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     6061 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     6121 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     6181 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     6241 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     6301 aagaccaccg cacagcaagc ggccggtgat cttcagacct ggacgatata tatgagggac
     6361 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     6421 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     6481 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     6541 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     6601 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     6661 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     6721 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     6781 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     6841 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     6901 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     6961 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     7021 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     7081 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     7141 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgccaa
     7201 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     7261 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     7321 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttggatcgat
     7381 aagcttgata tcgaattcct gcagccccga taaaataaaa gattttattt agtctccaga
     7441 aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagctgca gtaacgccat
     7501 tttgcaaggc atggaaaaat accaaaccaa gaatagagaa gttcagatca agggcgggta
     7561 catgaaaata gctaacgttg ggccaaacag gatatctgcg gtgagcagtt tcggccccgg
     7621 cccggggcca agaacagatg gtcaccgcag tttcggcccc ggcccgaggc caagaacaga
     7681 tggtccccag atatggccca accctcagca gtttcttaag acccatcaga tgtttccagg
     7741 ctcccccaag gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc agcctgcttc
     7801 tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa aagagctcac aacccctcac
     7861 tcggcgcgcc agtcctccga cagactgagt cgcccggggg ggatctggag ctctcgagaa
     7921 ttctcacgcg ccggatccgc caccatgtcc aaaaccatcg ttctttcggt cggcgaggct
     7981 actcgcactc tgactgagat ccagtccacc gcagaccgtc agatcttcga agagaaggtc
     8041 gggcctctgg tgggtcggct gcgcctcacg gcttcgctcc gtcaaaacgg agccaagacc
     8101 gcgtatcgcg tcaacctaaa actggatcag gcggacgtcg ttgattgctc caccagcgtc
     8161 tgcggcgagc ttccgaaagt gcgctacact caggtatggt cgcacgacgt gacaatcgtt
     8221 gcgaatagca ccgaggcctc gcgcaaatcg ttgtacgatt tgaccaagtc cctcgtcgcg
     8281 acctcgcagg tcgaagatct tgtcgtcaac cttgtgccgc tgggccgtcg tgcggacccg
     8341 ctagcctcct gcggccgctc caaaaccatc gttctttcgg tcggcgaggc tactcgcact
     8401 ctgactgaga tccagtccac cgcagaccgt cagatcttcg aagagaaggt cgggcctctg
     8461 gtgggtcggc tgcgcctcac ggcttcgctc cgtcaaaacg gagccaagac cgcgtatcgc
     8521 gtcaacctaa aactggatca ggcggacgtc gttgattgct ccaccagcgt ctgcggcgag
     8581 cttccgaaag tgcgctacac tcaggtatgg tcgcacgacg tgacaatcgt tgcgaatagc
     8641 accgaggcct cgcgcaaatc gttgtacgat ttgaccaagt ccctcgtcgc gacctcgcag
     8701 gtcgaagatc ttgtcgtcaa ccttgtgccg ctgggccgtc gtgcggaccc gctagcctcc
     8761 acgcgtgatt cgactagtgg aggaagcgga ggaggagtga gcaagggcga ggaggataac
     8821 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
     8881 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     8941 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
     9001 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
     9061 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     9121 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     9181 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     9241 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
     9301 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
     9361 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
     9421 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
     9481 ggcatggacg agctgtacaa gcatggcatg gatgagctct acaaaggtgg aggtcggacc
     9541 gaagagtaca agcttatcct gaacggtaaa accctgaaag gtgaaaccac caccgaagct
     9601 gttgacgctg ctaccgcgga aaaagttttc aaacagtacg ctaacgacaa cggtgttgac
     9661 ggtgaatgga cctacgacga cgctaccaaa accttcacgg taaccgaagc tagtggagga
     9721 agcggaggag gagtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
     9781 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     9841 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
     9901 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
     9961 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
    10021 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
    10081 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
    10141 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
    10201 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
    10261 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
    10321 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
    10381 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagcat
    10441 ggcatggatg agctctacaa aggtggaggt cggaccgaag agtacaagct tatcctgaac
    10501 ggtaaaaccc tgaaaggtga aaccaccacc gaagctgttg acgctgctac cgcggaaaaa
    10561 gttttcaaac agtacgctaa cgacaacggt gttgacggtg aatggaccta cgacgacgct
    10621 accaaaacct tcacggtaac cgaagctagt ggtggtggtg gtagcggtgg tggtagcagc
    10681 ttaccggtcg ccgaattcgg tgtgagcaag ggcgaggagg ataacatggc catcatcaag
    10741 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
    10801 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
    10861 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
    10921 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
    10981 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
    11041 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
    11101 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
    11161 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
    11221 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
    11281 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
    11341 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
    11401 tacaagcata tgtaagcggc cctttaagac caatgactta caaggcagct gtagatctta
    11461 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
    11521 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
    11581 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
    11641 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
    11701 cagtgtggaa aatctctagc agcatctaga attaattccg tgtattctat ag
//
