LOCUS Exported 2353 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Provides the A. thaliana U6-1 RNA polIII promoter as a level 0 GoldenBraid part. ACCESSION . VERSION . KEYWORDS pU6-1 (GB1204) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2353) AUTHORS Vazquez-Vilar M, Bernabe-Orts JM, Fernandez-Del-Carmen A, Ziarsolo P, Blanca J, Granell A, Orzaez D TITLE A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard. JOURNAL Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016. PUBMED 26839579 REFERENCE 2 (bases 1 to 2353) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2353 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 294..351 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." primer_bind complement(375..392) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(546..1134) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(626..645) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 1317..1411 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(1432..2091) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 2006..2025 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" primer_bind 2253..2271 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 2275..2318 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 2321..2342 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" ORIGIN 1 ctcgggaggt cattgtttta gttccaccac gattatattt gaaatttacg tgagtgtgag 61 tgagacttgc ataagaaaat aaaatcttta gttgggaaaa aattcaataa tataaatggg 121 cttgagaagg aagcgaggga taggcctttt tctaaaatag gcccatttaa gctattaaca 181 atcttcaaaa gtaccacagc gcttaggtaa agaaagcagc tgagtttata tatggttaga 241 gtcgaagtag tgattgtgag accactcatc gcgactagta gcggccgctg cagtccggca 301 aaaaagggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag attacttcgc 361 gttatgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 421 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 481 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 541 gcgtttttcc acaggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 601 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 661 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 721 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 781 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 841 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 901 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 961 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 1021 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 1081 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 1141 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 1201 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 1261 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagctcgag gcttggattc 1321 tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct 1381 gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc 1441 gccctgccac tcgtcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc 1501 atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt 1561 ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa 1621 atcaaaactg gtgaaactca cccagggatt ggctgatacg aaaaacatat tctcaataaa 1681 ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg 1741 tagaaactgc cggaaatcgt cgtggtattc actccagagt gatgaaaacg tttcagtttg 1801 ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt 1861 cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc 1921 cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg 1981 aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg 2041 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc 2101 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt 2161 atggtgaaag ttggaacctc ttacgtgccc gatcaactcg agtgccacct gacgtctaag 2221 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca 2281 gataaaaaaa atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag 2341 agcgatgagt ggt //