LOCUS       Exported                 853 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  I-SceI endonuclease expression vector with mammalian promoter to 
            introduce a DSB at a genomic I-SceI site.
ACCESSION   .
VERSION     .
KEYWORDS    pCBASceI
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Richardson C, Moynahan ME, Jasin M
  TITLE     Double-strand break repair by interchromosomal recombination: 
            suppression of chromosomal translocations.
  JOURNAL   Genes Dev. 1998 Dec 15. 12(24):3831-42.
  PUBMED    9869637
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     311..330
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    329..384
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     430..449
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
ORIGIN
        1 gggatctcca taagagaaga gggacagcta tgactgggag tagtcaggag aggaggaaaa
       61 atctggctag taaaacatgt aaggaaaatt ttagggatgt taaagaaaaa aataacacaa
      121 aacaaaatat aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga
      181 cagcaggggg ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag
      241 ccagcatatg ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat
      301 gatttgccct cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg
      361 caatgaaaat aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt
      421 ccccataatt tttggcagag ggaaaaagat ctcagtggta tttgtgagcc agggcattgg
      481 ccacaccagc caccaccttc tgataggcag cctgcacctg aggagtgaat tcctcgatcg
      541 acttattatt tcaggaaagt ttcggaggag atagtgttcg gcagtttgta catcatctgc
      601 gggatcaggt acggtttgat caggttgtag aagatcaggt aagacataga atcgatgtag
      661 atgatcggtt tgtttttgtt gatttttacg taacagttca gttggaattt gttacgcaga
      721 cccttaacca ggtattctac ttcttcgaaa gtgaaagact gggtgttcag tacgatcgat
      781 ttgttggtag agtttttgtt gtaatcccat ttaccaccat catccatgaa ccagtatgcc
      841 agagacatcg ggg
//
