LOCUS       Exported                 836 bp ds-DNA     linear   SYN 06-JUL-2021
DEFINITION  reprogrammed sortase A variant that recognizes alternative substrate
            LPXSG with high activity and specificity.
ACCESSION   .
VERSION     .
KEYWORDS    eSrtA(4S-9) pET29b
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Dorr BM, Ham HO, An C, Chaikof EL, Liu DR
  TITLE     Reprogramming the specificity of sortase enzymes.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Sep 16;111(37):13343-8. doi: 
            10.1073/pnas.1411179111. Epub 2014 Sep 3.
  PUBMED    25187567
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 6, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             492..514
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    529..553
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(553..572)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(554..572)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(660..679)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tagcagccgg atctcagtgg tggtggtggt ggtgggatcc tttcacttcg gtcgccacaa
       61 aaattttacg ggtttcccac acgccggttt caaagttata atcatcgcag gtcaccaggg
      121 tcagctgttt atctttgcct ttctgttcat ccagcacttc caccgcggtc ggtttcacgt
      181 tacgaatgct ggtcatttta tatttacggg tttcgttgcc cactttaaga tacaccatgc
      241 tgcctttttt cgccgccctc aggttggtaa actgatagtt cggacggtca atcgcggtat
      301 ggccggtaat gctaatgttc tgatcatcca ggctttcgtt ttcttccaca aagcacacac
      361 cacgatccag ctgttcacgg gtcgccgggc ccggatacac cggttcttta atatccgcat
      421 ccggaatttc aatatagccc gccactttgc ttttatcttt cggaatctgc ggtttcgcct
      481 gcatatgtat atctccttct taaagttaaa caaaattatt tctagagggg aattgttatc
      541 cgctcacaat tcccctatag tgagtcgtat taatttcgcg ggatcgagat cgatctcgat
      601 cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc
      661 ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc
      721 ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc
      781 cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgg
//
