LOCUS       Exported                6544 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Plasmid constitutively expressing phycocyanobilin (PCB) biosynthesis
            genes and UirS sensor kinase gene.
ACCESSION   .
VERSION     .
KEYWORDS    pPR220.08
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6544)
  AUTHORS   Ramakrishnan P, Tabor JJ
  TITLE     Repurposing Synechocystis PCC6803 UirS-UirR as a UV-violet/green 
            photoreversible transcriptional regulatory tool in E. coli.
  JOURNAL   ACS Synth Biol. 2016 Apr 27.
  PUBMED    27120220
REFERENCE   2  (bases 1 to 6544)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6544
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        2..35
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     RBS             52..63
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     RBS             795..806
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      1574..1645
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1661..1688
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             1888..2679
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     terminator      2691..2785
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      2899..3444
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     3345..3364
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3561..3578
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      6314..6385
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6401..6428
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 ctgacagcta gctcagtcct aggtataatg ctagctcaca cagaattcat taaagaggag
       61 aaaggtacca tgagtgtcaa cttagcttcc cagttgcggg aagggacgaa aaaatcccac
      121 tccatggcgg agaacgtcgg ctttgtcaaa tgcttcctca agggcgttgt cgagaaaaat
      181 tcctaccgta agctggttgg caatctctac tttgtctaca gtgccatgga agaggaaatg
      241 gcaaaattta aggaccatcc catcctcagc cacatttact tccccgaact caaccgcaaa
      301 caaagcctag agcaagacct gcaattctat tacggctcca actggcggca agaagtgaaa
      361 atttctgccg ctggccaagc ctatgtggac cgagtccggc aagtggccgc tacggcccct
      421 gaattgttgg tggcccattc ctacacccgt tacctggggg atctttccgg cggtcaaatt
      481 ctcaagaaaa ttgcccaaaa tgccatgaat ctccacgatg gtggcacagc tttctatgaa
      541 tttgccgaca ttgatgacga aaaggctttt aaaaatacct accgtcaagc tatgaatgat
      601 ctgcccattg accaagccac cgccgaacgg attgtggatg aagccaatga cgcctttgcc
      661 atgaacatga aaatgttcaa cgaacttgaa ggcaacctga tcaaggcgat cggcattatg
      721 gtgttcaaca gcctcacccg tcgccgcagt caaggcagca ccgaagttgg cctcgccacc
      781 tccgaaggct agttaaagag gagaaaggat ccatggccgt cactgattta agtttgacca
      841 attcttccct gatgcctacg ttgaacccga tgattcaaca gttggccctg gcgatcgccg
      901 ctagttggca aagtttaccc ctcaagccct atcaattgcc ggaggatttg ggctacgtag
      961 aaggccgcct ggaaggggaa aagttagtga ttgaaaatcg gtgctaccaa acgccccagt
     1021 ttcgcaaaat gcatttggag ttggccaagg tgggcaaagg gttggatatt ctccactgtg
     1081 taatgtttcc tgagccttta tacggtctac ctttgtttgg ctgtgacatt gtggccggcc
     1141 ccggtggagt aagtgcggct attgcggatc tatcccccac ccaaagcgat cgccaattgc
     1201 ccgcagcgta ccaaaaatca ttggcagagc taggccagcc agaatttgag caacaacggg
     1261 aattgccccc ctggggagaa atattttctg aatattgttt attcatccgt cccagcaatg
     1321 tcactgaaga agaaagattt gtacaaaggg tagtggactt tttgcaaatt cattgtcacc
     1381 aatccatcgt tgccgaaccc ttgtctgaag ctcaaacttt ggagcaccgt caggggcaaa
     1441 ttcattactg ccaacaacaa cagaaaaatg ataaaacccg tcgggtactg gaaaaagctt
     1501 ttggggaagc ttgggcggaa cggtatatga gccaagtctt atttgatgtt atccaataat
     1561 ctagaccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     1621 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
     1681 tttctgcgtt tataggtacc cggggatcct ctagagtcga cctgcaggca tgcaagctta
     1741 gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa gccctgcaaa
     1801 gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc aagatctgat
     1861 caagagacag gatgaggatc gtttcgcatg agggaagcgg tgatcgccga agtatcgact
     1921 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
     1981 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg
     2041 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
     2101 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
     2161 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
     2221 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
     2281 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
     2341 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
     2401 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
     2461 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     2521 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
     2581 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac
     2641 tacgtgaaag gcgagatcac caaggtagtc ggcaaataag agctcgcttg gactcctgtt
     2701 gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg
     2761 ccgggcgttt tttattggtg agaatccaag cactagtaac aacttatatc gtatggggct
     2821 gacttcaggt gctacatttg aagagataaa ttgcactgaa atctagtaat attttatctg
     2881 attaataaga tgatcttctt gagatcgttt tggtctgcgc gtaatctctt gctctgaaaa
     2941 cgaaaaaacc gccttgcagg gcggtttttc gaaggttctc tgagctacca actctttgaa
     3001 ccgaggtaac tggcttggag gagcgcagtc accaaaactt gtcctttcag tttagcctta
     3061 accggcgcat gacttcaaga ctaactcctc taaatcaatt accagtggct gctgccagtg
     3121 gtgcttttgc atgtctttcc gggttggact caagacgata gttaccggat aaggcgcagc
     3181 ggtcggactg aacggggggt tcgtgcatac agtccagctt ggagcgaact gcctacccgg
     3241 aactgagtgt caggcgtgga atgagacaaa cgcggccata acagcggaat gacaccggta
     3301 aaccgaaagg caggaacagg agagcgcacg agggagccgc cagggggaaa cgcctggtat
     3361 ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc gtcagatttc gtgatgcttg
     3421 tcaggggggc ggagcctatg gaaaaacggc tttgccgcgg ccctctcact tccctgttaa
     3481 gtatcttcct ggcatcttcc aggaaatctc cgccccgttc gtaagccatt tccgctcgcc
     3541 gcagtcgaac gaccgagcgt agcgagtcag tgagcgagga agcggaatat atcccgaagc
     3601 ggcatgcatt tacgttgaca ccatcgaatg ctgattgacg gctagctcag tcctaggtat
     3661 agtgctagcc agtgggccca agttcactta aaaaggagat caacaatgaa agcaattttc
     3721 gtactgaaac atcttaatca tgctttggag tttttctgat ggcaatcacc gcatttaccc
     3781 tgggggattt tttccaagcc aacagctata taccccacgg ccattgctac ctttggcaaa
     3841 ctcccctggt ctggctccac gtcagcgctg attttttcac ggcgatcgcc tactactcta
     3901 ttcccttaac tctgctctac tttttgcgga agcgccaaga tattcctttt cctaatatta
     3961 ttttcctgtt tagtactttt attttatgct gtggcaccag tcactttttt gacattatca
     4021 ccctttggta ccctatttat tggatatccg gtacagtcaa agcctccatg gcgatcgtct
     4081 ctattatcac tgtgtttgaa ttaattcaaa ttgtacccaa tgccctaaat ttaaaatccc
     4141 ccacggaatt agctaccctc aatctggctc taaatcaaga aattaaagaa aggcaaacag
     4201 cggaaatagc attgcaggag ttaaacaata atcttgaaaa aagagtggaa gaccgcacta
     4261 cccaattagc aaaaataaat caacaattag aacaggaaat tgaagataaa acccgggcaa
     4321 aagaagatct agaaaaaaat aaagatcaat tggcgcaatt ggctgctatt gtcgaatctt
     4381 cccaggacgc aattattagt aaaaccctag atggtaacat caccagttgg aacgagtctg
     4441 cggaaagatt atttggctac acagcagaag aaatgattgg aagtcatatc accaaactta
     4501 ttcctgagga attaatcttg gaagaagacc ttattgctga atgcatccgc caaggtcaga
     4561 gaattaacac ctacgaaact caaagacagc gtaaagatgg tactaaaatt gatgttgccc
     4621 tgaccatttc tcccattcga gatgaacaca aaaatgtagt gggagcctcc aaaattgtta
     4681 gggatattac cgccagattg gatgtggaaa atgccctacg ggaaagtcaa tattttattg
     4741 aaaaattagc taattattct ccccagattc tttatatttt agaccccatt gcttggaaaa
     4801 atatttatgt caattatcaa tccttcgaaa tcctaggcta cactccagaa gaatttaaaa
     4861 atgggggaac ggagctgttg ttaaatattg tgcatcccga tgacatccca accctttacg
     4921 aaaataagaa tttttggcaa aagtcccagg aaggccaagt gttgaccact gaatatcgaa
     4981 tgcgccacaa aaatggttct tggcggtggc tacgttctcg ggaagtggtt tttgctaggg
     5041 atgattatgg gcaagttact aaggttttgg gcacagccca ggatattagt gatagcaaag
     5101 aacaggaaca aagactttat gagcaaggcc gccgcgaatc attattaagg gaaattaccc
     5161 agcgtatccg tcagtcttta gatctgccaa ctatttttaa cacagtagtg caggaaattc
     5221 gtcagttttt ggaagcagat cgggtagtta tttttcagtt ttcacccgac tctgactttt
     5281 ccgttggtaa tattgtggca gagtcggtat tggctccatt taagccaatc attaatagtg
     5341 caattgaaga aacttgtttt agtaataact atgcccaaag gtatcagcag ggcagaattc
     5401 aggtcattga ggatattcac cagtcccatc ttaggcaatg ccacattgac tttcttgcca
     5461 ggctacaggt cagggcaaac ctagtgctac cactaattaa tgatgccatt ttgtggggct
     5521 tattgtgtat tcatcaatgt gacagttcta gagtttggga acaaacagaa attgatctgc
     5581 tcaagcagat cactaatcag tttgaaattg ccatccaaca ggctactttg tacgagcagg
     5641 ctcaacaaga attggcttct aaaaatcagc tttttgtaca attaaccaac gaattagagc
     5701 aaaaaaaagt attgcttaag gaaattcatc atcgagttaa aaacaatttg caaattatgt
     5761 ctagtttact ttatttgcaa tttagtaagg cttctccagc catccagcaa ttgagtgagg
     5821 aataccagaa tcgcattcaa tccatggcat tgattcatga gcagttatat cgtagtgaag
     5881 acttagctaa tattgatttt agtcaatatc ttaaaaattt gactcacaat atctgccaaa
     5941 gttacggctg taatactgat agtattaaaa ttaaattgtt ggttgaacaa gttaaagttc
     6001 ctttagagca gtccattcct ttgggattaa ttattcagga attagtttcc aatgctttaa
     6061 aacatgcttt cccaacgacg gaaggggaaa tttcgattaa attcacttca atgaacagtc
     6121 actatagttt acaggtgtgg gataacggag ttggaatttc cagggatata gatttggaaa
     6181 atacagatag tttgggcatg caacttatct acagcctaac tgaacaactc cagggagaat
     6241 tgcactatga atatgtcggt ggcgctcaat ttgggttgga attttcactt tagtaataat
     6301 ctagaccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     6361 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
     6421 tttctgcgtt tatagtaaat cactgcataa ttcgtgtcga ttaaaccgcc taagtccccc
     6481 aggaaagggg gatataacag tatagatttt gtcagccttc agcttggctt taccgtcaaa
     6541 aaaa
//
