LOCUS       Exported                 827 bp ds-DNA     linear   SYN 21-MAY-2021
DEFINITION  Mammalian expression of miR34.
ACCESSION   .
VERSION     .
KEYWORDS    miR34a in pcDNA3.1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Eichelser C, Stuckrath I, Muller V, Milde-Langosch K, Wikman H, 
            Pantel K, Schwarzenbach H
  TITLE     Increased serum levels of circulating exosomal microRNA-373 in 
            receptor-negative breast cancer patients.
  JOURNAL   Oncotarget. 2014 Oct 30;5(20):9650-63.
  PUBMED    25333260
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 21, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(335..352)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    341..565
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(698..717)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact
       61 cactataggg agacccaagc tggctagcgt ttaaacttaa gcttggtacc gagctcggat
      121 ccactagtcc agtgtggtgg aattctgcag atatccagca cagtggcggc cgctcgagat
      181 ctggccagct gtgagtgttt ctttggcagt gtcttagctg gttgttgtga gcaatagtaa
      241 ggaagcaatc agcaagtata ctgccctaga agtgctgcac gttgtggggc ccggatcctc
      301 gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
      361 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
      421 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
      481 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
      541 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
      601 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
      661 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
      721 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
      781 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatta
//
