LOCUS Exported 847 bp ds-DNA linear SYN 19-MAY-2021 DEFINITION sgRNA1. ACCESSION . VERSION . KEYWORDS pLH-sgRNA1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 847) AUTHORS Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T TITLE Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. JOURNAL Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526. PUBMED 27088723 REFERENCE 2 (bases 1 to 847) AUTHORS . TITLE Direct Submission JOURNAL Exported May 19, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..847 /organism="synthetic DNA construct" /mol_type="other DNA" misc_RNA complement(72..147) /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" CDS complement(168..473) /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind complement(270..289) /label=ccdB-fwd /note="ccdB gene, forward primer" ORIGIN 1 caatcccccc ttttctttta aaattgtgga tgaatactgc catttgtctc gaggtcgaga 61 attcaaaaaa agcaccgact cggtgccact ttttcaagtt gataacggac tagccttatt 121 tgaacttgct atttctagct ctcaaacggg tcttcgtcct gcagggttta tattccccag 181 aacatcaggt taatggcgtt tttgatgtca ttttcgcggt ggctgagatc agccacttct 241 tccccgataa cggagaccgg cacactggcc atatcggtgg tcatcatgcg ccagctttca 301 tccccgatat gcaccaccgg gtaaagttca cgggagactt tatctgacag cagacgtgca 361 ctggccaggg ggatcaccat ccgtcgcccg ggcgtgtcaa taatatcact ctgtacatcc 421 acaaacagac gataacggct ctctctttta taggtgtaaa ccttaaactg catttcacca 481 gtccctgttc tcgtcagcaa aagagccgtt catttcaata aaccgggcga cctcagccat 541 cccttcctga ttttccgctt tccagcgttc ggcacgcaga cgacgggctt cattctgcat 601 ggttgtgctt accagaccgg agatattgac atcatatatg ccttgagcaa ctgatagctg 661 tcgctgtcaa ctgtcactgt aatacgctgc ttcatagcac acctcttttt gacatacttc 721 gggtatacat atcagtatat attcttatac cgcaaaaatc agcgcgcaaa tacgcatact 781 gttatctggc ttttagtaag cctcgaagac accggtgttt cgtcctttcc acaagatata 841 taaagcc //