LOCUS       Exported                 830 bp ds-DNA     linear   SYN 06-SEP-2021
DEFINITION  pp32(1-149) cloned into pProEX HTb.
ACCESSION   .
VERSION     .
KEYWORDS    pp32-Delta-CT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Huyton T, Wolberger C
  TITLE     The crystal structure of the tumor suppressor protein pp32 (Anp32a):
            structural insights into Anp32 family of proteins.
  JOURNAL   Protein Sci. 2007 Jul;16(7):1308-15. Epub 2007 Jun 13.
  PUBMED    17567741
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 6, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             1..21
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(540..557)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      710..798
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gaaaacctgt attttcaggg cgccatggga tccatggaga tgggcagacg gattcattta
       61 gagctgcgga acaggacgcc ctctgatgtg aaagaacttg tcctggacaa cagtcggtcg
      121 aatgaaggca aactcgaagg cctcacagat gaatttgaag aactggaatt cttaagtaca
      181 atcaacgtag gcctcacctc aatcgcaaac ttaccaaagt taaacaaact taagaagctt
      241 gaactaagcg ataacagagt ctcagggggc ctggaagtat tggcagaaaa gtgtccgaac
      301 ctcacgcatc taaatttaag tggcaacaaa attaaagacc tcagcacaat agagccactg
      361 aaaaagttag aaaacctcaa gagcttagac cttttcaatt gcgaggtaac caacctgaac
      421 gactaccgag aaaatgtgtt caagctcctc ccgcaactca catatctcga cggctatgac
      481 taactcgagg catgcggtac caagcttggc tgttttggcg gatgagagaa gattttcagc
      541 ctgatacaga ttaaatcaga acgcagaagc ggtctgataa aacagaattt gcctggcggc
      601 agtagcgcgg tggtcccacc tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc
      661 gatggtagtg tggggtctcc ccatgcgaga gtagggaact gccaggcatc aaataaaacg
      721 aaaggctcag tcgaaagact gggccttttc gttttatctg ttgtttgtcg gtgaacgcct
      781 ctcctgagta ggacaaatcc cgccggggag cggatttgaa acgttgcgaa
//
