LOCUS       Exported                 863 bp ds-DNA     linear   SYN 20-MAY-2021
DEFINITION  Expresses biosensor for cAMP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    Flamindo2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Odaka H, Arai S, Inoue T, Kitaguchi T
  TITLE     Genetically-encoded yellow fluorescent cAMP indicator with an 
            expanded dynamic range for dual-color imaging.
  JOURNAL   PLoS One. 2014 Jun 24;9(6):e100252. doi: 
            10.1371/journal.pone.0100252. eCollection 2014.
  PUBMED    24959857
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 20, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(2..250)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELY"
     primer_bind     complement(41..62)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca
       61 tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga ctggtagctc aggtagtggt
      121 tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga
      181 gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg atgccgttct
      241 tctgcttgtc ggccatgata tagacgttgt ggctgttgaa ttcttcttcc agtctcatgg
      301 tttttgcttc cacatccttg atgatgcggt tgaagtcctg cttgtccaca cgcagaaagt
      361 gacagttatt ttctcgaagg atgatggtgg ctgcccgagg tgcgtcgttc accagagcca
      421 gctgtccaaa gtcatctccc tcgtgcaacg tggtcaccag ccccttgcca tgggtcacca
      481 cattgacaga tcccttccag ataatgtacc acgaggtacc cttgtccccc tggctgaaca
      541 acacagttcc tgccttgctg tgtggttcaa agagcagaac agcagctagt tcccgcttca
      601 ccgagttaga aaggtgtgcc accgccttga tatgcagcag ctcttcaaag atcaggtcca
      661 gttcttcatc cgtacgctga cctgggggct tccggagcgc cacggtgagt agggcatcag
      721 gccctcgctg ggacaggagg gccatagcct caacaagctc ctctttcttc agtgccccgc
      781 ggaggttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct
      841 tcagctcgat gcggttcacc agg
//
