LOCUS Exported 850 bp ds-DNA linear SYN 21-MAY-2021 DEFINITION Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG. ACCESSION . VERSION . KEYWORDS VP12 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 850) AUTHORS Kleinstiver BP, Pattanayak V, Prew MS, Tsai SQ, Nguyen NT, Zheng Z, Keith Joung J TITLE High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects. JOURNAL Nature. 2016 Jan 6. doi: 10.1038/nature16526. PUBMED 26735016 REFERENCE 2 (bases 1 to 850) AUTHORS . TITLE Direct Submission JOURNAL Exported May 21, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..850 /organism="synthetic DNA construct" /mol_type="other DNA" ORIGIN 1 aagcagcgga ctttcgacaa cggtagcatt ccacatcaaa tccacttagg cgaattgcat 61 gctatactta gaaggcagga ggatttttat ccgttcctca aagacaatcg tgaaaagatt 121 gagaaaatcc taacctttcg cataccttac tatgtgggac ccctggcccg agggaactct 181 cggttcgcat ggatgacaag aaagtccgaa gaaacgatta ctccctggaa ttttgaggaa 241 gttgtcgata aaggtgcgtc agctcaatcg ttcatcgaga ggatgaccgc ctttgacaag 301 aatttaccga acgaaaaagt attgcctaag cacagtttac tttacgagta tttcacagtg 361 tacaatgaac tcacgaaagt taagtatgtc actgagggca tgcgtaaacc cgcctttcta 421 agcggagaac agaagaaagc aatagtagat ctgttattca agaccaaccg caaagtgaca 481 gttaagcaat tgaaagagga ctactttaag aaaattgaat gcttcgattc tgtcgagatc 541 tccggggtag aagatcgatt taatgcgtca cttggtacgt atcatgacct cctaaagata 601 attaaagata aggacttcct ggataacgaa gagaatgaag atatcttaga agatatagtg 661 ttgactctta ccctctttga agatcgggaa atgattgagg aaagactaaa aacatacgct 721 cacctgttcg acgataaggt tatgaaacag ttaaagaggc gtcgctatac gggctgggga 781 gccttgtcgc ggaaacttat caacgggata agagacaagc aaagtggtaa aactattctc 841 gattttctaa //