LOCUS Exported 8775 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION DULIP Prey Destination Vector (Gateway, C-terminal). ACCESSION . VERSION . KEYWORDS pGW-FL-V5 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8775) AUTHORS Trepte P, Buntru A, Klockmeier K, Willmore L, Arumughan A, Secker C, Zenkner M, Brusendorf L, Rau K, Redel A, Wanker EE TITLE DULIP: A Dual Luminescence-Based Co-Immunoprecipitation Assay for Interactome Mapping in Mammalian Cells. JOURNAL J Mol Biol. 2015 Oct 23;427(21):3375-88. doi: 10.1016/j.jmb.2015.08.003. Epub 2015 Aug 8. PUBMED 26264872 REFERENCE 2 (bases 1 to 8775) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8775 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(44..63) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 769..789 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 863..882 /label=T7 /note="T7 promoter, forward primer" promoter 863..881 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 901..1025 /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 1050..1080 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" CDS 1134..1793 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(1200..1219) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" CDS 2135..2440 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind 2319..2338 /label=ccdB-fwd /note="ccdB gene, forward primer" protein_bind complement(2481..2605) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" CDS 2613..4262 /codon_start=1 /gene="luc+" /product="firefly luciferase" /label=luciferase /note="enhanced luc+ version of the luciferase gene" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV" primer_bind complement(2679..2697) /label=LucNrev /note="Firefly luciferase, reverse primer" CDS 4287..4328 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" primer_bind complement(4369..4386) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 4375..4599 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 4645..5073 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4732..4751) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4942..4963 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(5082..5102) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 5087..5416 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 5267..5402 /label=SV40 ori /note="SV40 origin of replication" primer_bind 5329..5348 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 5483..6277 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(5537..5556) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 6147..6166 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 6451..6572 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(6488..6507) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 6542..6561 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(6621..6637) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6621..6637) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(6634..6656) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 6645..6661 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6669..6699) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6714..6735 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(6852..6869) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7023..7608) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7103..7122) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7779..8639) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8402..8421 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8640..8744) /gene="bla" /label=AmpR promoter ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta 961 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca 1021 ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg 1081 tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga 1141 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg 1201 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg 1261 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc 1321 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg 1381 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt 1441 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag 1501 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt 1561 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata 1621 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg 1681 tgctgatgcc gctggcgatt caggttcatc atgccgtctg tgatggcttc catgtcggca 1741 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taatctagag 1801 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta 1861 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtgtg ctatgaagca 1921 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc 1981 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa 2041 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac 2101 ggctcttttg ctgacgagaa cagggactgg tgaaatgcag tttaaggttt acacctataa 2161 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg 2221 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga 2281 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc 2341 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga 2401 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca 2461 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag 2521 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc 2581 gttcagcttt cttgtacaaa gtggtgaagc ttatggaaga cgccaaaaac ataaagaaag 2641 gcccggcgcc attctatccg ctggaagatg gaaccgctgg agagcaactg cataaggcta 2701 tgaagagata cgccctggtt cctggaacaa ttgcttttac agatgcacat atcgaggtgg 2761 acatcactta cgctgagtac ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat 2821 atgggctgaa tacaaatcac agaatcgtcg tatgcagtga aaactctctt caattcttta 2881 tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac gacatttata 2941 atgaacgtga attgctcaac agtatgggca tttcgcagcc taccgtggtg ttcgtttcca 3001 aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaagct cccaatcatc caaaaaatta 3061 ttatcatgga ttctaaaacg gattaccagg gatttcagtc gatgtacacg ttcgtcacat 3121 ctcatctacc tcccggtttt aatgaatacg attttgtgcc agagtccttc gatagggaca 3181 agacaattgc actgatcatg aactcctctg gatctactgg tctgcctaaa ggtgtcgctc 3241 tgcctcatag aactgcctgc gtgagattct cgcatgccag agatcctatt tttggcaatc 3301 aaatcattcc ggatactgcg attttaagtg ttgttccatt ccatcacggt tttggaatgt 3361 ttactacact cggatatttg atatgtggat ttcgagtcgt cttaatgtat agatttgaag 3421 aagagctgtt tctgaggagc cttcaggatt acaagattca aagtgcgctg ctggtgccaa 3481 ccctattctc cttcttcgcc aaaagcactc tgattgacaa atacgattta tctaatttac 3541 acgaaattgc ttctggtggc gctcccctct ctaaggaagt cggggaagcg gttgccaaga 3601 ggttccatct gccaggtatc aggcaaggat atgggctcac tgagactaca tcagctattc 3661 tgattacacc cgagggggat gataaaccgg gcgcggtcgg taaagttgtt ccattttttg 3721 aagcgaaggt tgtggatctg gataccggga aaacgctggg cgttaatcaa agaggcgaac 3781 tgtgtgtgag aggtcctatg attatgtccg gttatgtaaa caatccggaa gcgaccaacg 3841 ccttgattga caaggatgga tggctacatt ctggagacat agcttactgg gacgaagacg 3901 aacacttctt catcgttgac cgcctgaagt ctctgattaa gtacaaaggc tatcaggtgg 3961 ctcccgctga attggaatcc atcttgctcc aacaccccaa catcttcgac gcaggtgtcg 4021 caggtcttcc cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg 4081 gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta acaaccgcga 4141 aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac 4201 tcgacgcaag aaaaatcaga gagatcctca taaaggccaa gaagggcgga aagatcgccg 4261 tggacgcgaa ttccgcgggg ctcgacggta agcctatccc taaccctctc ctcggtctcg 4321 attctacgtg actcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc 4381 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag 4441 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta 4501 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag 4561 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca 4621 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg 4681 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg 4741 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg 4801 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt 4861 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt 4921 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta 4981 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa 5041 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg 5101 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 5161 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 5221 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac 5281 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga 5341 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg 5401 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga 5461 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc 5521 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga 5581 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct 5641 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac 5701 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct 5761 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt 5821 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt 5881 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt 5941 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag 6001 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt 6061 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg 6121 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg 6181 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg 6241 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg 6301 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat 6361 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg 6421 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac 6481 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 6541 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc 6601 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 6661 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 6721 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 6781 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 6841 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 6901 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 6961 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 7021 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 7081 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 7141 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 7201 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 7261 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 7321 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 7381 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 7441 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt 7501 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 7561 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 7621 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 7681 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 7741 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 7801 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 7861 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 7921 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 7981 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 8041 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 8101 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 8161 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 8221 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 8281 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 8341 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 8401 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 8461 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 8521 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 8581 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 8641 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 8701 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 8761 gtgccacctg acgtc //