LOCUS       Exported                 839 bp ds-DNA     linear   SYN 21-MAY-2021
DEFINITION  sgRNA1-2XPP7.
ACCESSION   .
VERSION     .
KEYWORDS    pLH-sgRNA1-2XPP7
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T
  TITLE     Multiplexed labeling of genomic loci with dCas9 and engineered 
            sgRNAs using CRISPRainbow.
  JOURNAL   Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
  PUBMED    27088723
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 21, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    250..367
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa
       61 ccctgcagga cgaagacccg tttgagagct accggagcag acgatatggc gtcgctccgg
      121 tagcaagttc aaataaggct agtccgttat caacttggag cagacgatat ggcgtcgctc
      181 caagtggcac cgagtcggtg ctttttttga attctcgacc tcgagacaaa tggcagtatt
      241 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
      301 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
      361 aaattttcgg gtttattaca gggacagcag agatccactt tggccgcggc tcgagggggt
      421 tggggttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc
      481 gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc
      541 gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc
      601 cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag
      661 ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc
      721 gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag agcagcggcc
      781 gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgccc
//
