LOCUS Exported 4475 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Figure 2. Expresses BxbI.. ACCESSION . VERSION . KEYWORDS PP43 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4475) AUTHORS Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, Ram R, Lu TK TITLE Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. JOURNAL Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211. PUBMED 27470089 REFERENCE 2 (bases 1 to 4475) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4475 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 56..464 /gene="S. cerevisiae TEF1" /label=TEF1 promoter /note="promoter for EF-1-alpha" primer_bind complement(2001..2019) /label=CYC1 /note="CYC1 transcription termination signal, reverse primer" terminator 2031..2220 /gene="S. cerevisiae CYC1" /label=CYC1 terminator /note="transcription terminator for CYC1" primer_bind complement(2299..2316) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 2305..2529 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" CDS 2702..3496 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2756..2775) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3366..3385 /label=Neo-F /note="Neomycin resistance gene, forward primer" rep_origin 3822..4410 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4311..4330 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 gactcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagtccaca 61 caccatagct tcaaaatgtt tctactcctt ttttactctt ccagattttc tcggactccg 121 cgcatcgccg taccacttca aaacacccaa gcacagcata ctaaatttcc cctctttctt 181 cctctagggt gtcgttaatt acccgtacta aaggtttgga aaagaaaaaa gagaccgcct 241 cgtttctttt tcttcgtcga aaaaggcaat aaaaattttt atcacgtttc tttttcttga 301 aaattttttt tttttgattt ttttctcttt cgatgacctc ccattgatat ttaagttaat 361 aaacggtctt caatttctca agtttcagtt tcatttttct tgttctatta caactttttt 421 tacttcttgc tcattagaaa gaaagcatag caatctaatc taaggctagc gtttaaacca 481 ccatgagggc ccttgtagtt atcaggttga gtagggttac ggatgcaacc accagcccgg 541 agcgccaact ggaatcatgt cagcagcttt gtgcgcagcg cggctgggac gtggtgggag 601 tggcggagga cttggacgtt agcggggccg ttgacccatt tgaccgaaag cggagaccta 661 acctggctcg atggctcgcc tttgaggagc agcccttcga tgtgatcgtc gcatacaggg 721 tcgacagact gaccagatcc attcgccatc tgcagcagct cgttcactgg gcggaggacc 781 acaaaaagct cgtggtgagt gcaacagaag cccactttga caccacaaca cccttcgcag 841 ccgtcgtgat cgctctgatg ggtaccgttg cccagatgga attggaggca atcaaggagc 901 ggaacagatc cgccgctcat ttcaatatcc gcgcgggcaa gtacaggggt agtctcccac 961 cctgggggta tttgcctacc cgggtggacg gcgaatggag gcttgttccc gatcccgtgc 1021 agcgagagcg aatactggaa gtttatcatc gagtcgtgga taaccatgaa ccactccacc 1081 tggtggccca cgaccttaac cgacgcggcg tgctgagccc taaggactat tttgctcaac 1141 ttcagggaag agagccacag ggtagggaat ggtcagccac agctctcaag cggtctatga 1201 tttccgaagc aatgctcggg tacgcaacac tcaatggcaa gacagttcga gacgacgacg 1261 gggcccccct ggttcgggcc gaacccatac ttacccgcga acaactggag gcacttcgcg 1321 cggaacttgt gaaaacaagc cgagccaaac ccgcagtgag caccccatca ctgctgctga 1381 gggtgctctt ctgtgccgtg tgcggcgaac cagcatacaa gttcgctggc gggggtcgaa 1441 aacacccccg ctaccggtgt cgctcaatgg gttttccaaa gcactgtggc aacggaacag 1501 ttgcaatggc cgaatgggac gctttttgtg aagaacaagt gctggatctt ctgggcgacg 1561 ctgagaggct ggaaaaagta tgggtggccg ggagcgacag cgccgttgag ctcgccgagg 1621 tgaacgccga attggtggac ctgacgagtc tcatcggatc tccagcatac cgagctggat 1681 ccccccagcg agaggctctg gacgctcgga tagccgccct ggcagcaagg caggaggagc 1741 ttgaggggtt ggaagcacgg ccttcaggat gggaatggcg ggaaacagga cagagatttg 1801 gagactggtg gagggaacag gataccgctg ctaagaacac ttggctcagg tccatgaatg 1861 ttcgactcac cttcgacgtg aggggtgggt tgacccgcac cattgatttc ggggatctgc 1921 aggagtatga acagcatctc cggcttggct ccgtggtaga aagacttcat acaggcatgt 1981 catgaagatc tattagttat gtcacgctta cattcacgcc ctccccccac atccgctcta 2041 accgaaaagg aaggagttag acaacctgaa gtctaggtcc ctatttattt ttttatagtt 2101 atgttagtat taagaacgtt atttatattt caaatttttc ttttttttct gtacagacgc 2161 gtgtacgcat gtaacattat actgaaaacc ttgcttgaga aggttttggg acgctcgaag 2221 gctttaattt gcaagctgga gaccaacatg tgagcaaaag gccagcatct agagggcccg 2281 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc 2341 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa 2401 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg 2461 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg 2521 gctctatggc ttctactggg cggttttatg gacagcaagc gaaccggaat tgccagctgg 2581 ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt tctcgccgcc 2641 aaggatctga tggcgcaggg gatcaagctc tgatcaagag acaggatgag gatcgtttcg 2701 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt 2761 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc 2821 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact 2881 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt 2941 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca 3001 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat 3061 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg 3121 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga 3181 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga 3241 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa 3301 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga 3361 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt 3421 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct 3481 tgacgagttc ttctgaatta ttaacgctta caatttcctg atgcggtatt ttctccttac 3541 gcatctgtgc ggtatttcac accgcataca ggtggcactt ttcggggaaa tgtgcgcgga 3601 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa 3661 ccctgataaa tgcttcaata atagcacgtg ctaaaacttc atttttaatt taaaaggatc 3721 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 3781 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 3841 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 3901 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 3961 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 4021 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 4081 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 4141 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 4201 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 4261 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 4321 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 4381 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 4441 ctgggctttt gctggccttt tgctcacatg ttctt //