LOCUS       Exported                8748 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses APEX in the mitochondrial intermembrane space..
ACCESSION   .
VERSION     .
KEYWORDS    IMS-APEX2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8748)
  AUTHORS   Hung V, Zou P, Rhee HW, Udeshi ND, Cracan V, Svinkina T, Carr SA, 
            Mootha VK, Ting AY
  TITLE     Proteomic mapping of the human mitochondrial intermembrane space in 
            live cells via ratiometric APEX tagging.
  JOURNAL   Mol Cell. 2014 Jul 17;55(2):332-41. doi: 
            10.1016/j.molcel.2014.06.003. Epub 2014 Jul 4.
  PUBMED    25002142
REFERENCE   2  (bases 1 to 8748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8748
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        65..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     523..543
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    595..619
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             850..891
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             892..1638
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     protein_bind    complement(1646..1670)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1672..1713
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    1755..2343
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1808..1828)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2226..2237)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2415..2648
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2726..2847
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2763..2782)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2817..2836
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2887..3022
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2949..2968
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(3042..3061)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3043..3061)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3071..3088)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3071..3087)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3080..3102)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3229..3684
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3316..3335)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3526..3547
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3710..3814
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3815..4675
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4033..4052)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4846..5434
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5335..5354
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5588..5605
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5722..5743
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5758..5788
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5796..5812
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5801..5823
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5820..5836
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5820..5836
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5855..5875
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5857..5875
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5903..6129
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6130..6310
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6357..6482
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6975..7208
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7393..7437
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7612..8112
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8031..8049
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8124..8519
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8579..8696
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt
      601 ttgtacaaaa aagcaggctt cgaaggagat agaaccatgt accggctcct gtcaagcgtg
      661 acagctcggg ctgcggccac cgcaggccca gcctgggacg gagggcggcg cggggcgcac
      721 aggcgaccgg gcctgcctgt gctgggcctt ggttgggccg gcggcctggg gctcgggctg
      781 gggctggctc tcggcgcgaa gctggtggtc gggctgcggg gcgccgtccc cattcagtcc
      841 agggatccag gcaagcccat ccccaacccc ctgctgggcc tggacagcac cggaaagtct
      901 tacccaactg tgagtgctga ttaccaggac gccgttgaga aggcgaagaa gaagctcaga
      961 ggcttcatcg ctgagaagag atgcgctcct ctaatgctcc gtttggcatt ccactctgct
     1021 ggaacctttg acaagggcac gaagaccggt ggacccttcg gaaccatcaa gcaccctgcc
     1081 gaactggctc acagcgctaa caacggtctt gacatcgctg ttaggctttt ggagccactc
     1141 aaggcggagt tccctatttt gagctacgcc gatttctacc agttggctgg cgttgttgcc
     1201 gttgaggtca cgggtggacc taaggttcca ttccaccctg gaagagagga caagcctgag
     1261 ccaccaccag agggtcgctt gcccgatccc actaagggtt ctgaccattt gagagatgtg
     1321 tttggcaaag ctatggggct tactgaccaa gatatcgttg ctctatctgg gggtcacact
     1381 attggagctg cacacaagga gcgttctgga tttgagggtc cctggacctc taatcctctt
     1441 attttcgaca actcatactt cacggagttg ttgagtggtg agaaggaagg tctccttcag
     1501 ctaccttctg acaaggctct tttgtctgac cctgtattcc gccctctcgt tgataaatat
     1561 gcagcggacg aagatgcctt ctttgctgat tacgctgagg ctcaccaaaa gctttccgag
     1621 cttgggtttg ctgatgcctg ataagaccca gctttcttgt acaaagtggt tggtaagcct
     1681 atccctaacc ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg
     1741 cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     1801 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     1861 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     1921 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     1981 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     2041 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     2101 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     2161 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     2221 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     2281 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     2341 cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     2401 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     2461 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     2521 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     2581 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     2641 ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     2701 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     2761 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     2821 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     2881 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     2941 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     3001 tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt
     3061 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     3121 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     3181 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     3241 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     3301 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     3361 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     3421 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     3481 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     3541 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     3601 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     3661 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     3721 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     3781 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     3841 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     3901 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     3961 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     4021 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     4081 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     4141 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     4201 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     4261 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     4321 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     4381 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     4441 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     4501 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     4561 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     4621 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     4681 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     4741 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     4801 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     4861 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     4921 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     4981 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     5041 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     5101 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     5161 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     5221 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     5281 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     5341 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     5401 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     5461 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     5521 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     5581 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     5641 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     5701 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     5761 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     5821 aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca
     5881 aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg
     5941 gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg
     6001 gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg
     6061 attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga
     6121 tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     6181 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     6241 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     6301 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     6361 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     6421 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     6481 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     6541 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     6601 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     6661 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     6721 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     6781 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct
     6841 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     6901 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     6961 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     7021 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     7081 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     7141 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     7201 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     7261 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     7321 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     7381 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     7441 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     7501 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     7561 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cttgcgcctt
     7621 ttccaaggca gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa
     7681 cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg
     7741 atcttcgccg ctacccttgt gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg
     7801 gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact
     7861 agtaccctcg cagacggaca gcgccaggga gcaatggcag cgcgccgacc gcgatgggct
     7921 gtggccaata gcggctgctc agcagggcgc gccgagagca gcggccggga aggggcggtg
     7981 cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca
     8041 ttctgcaagc ctccggagcg cacgtcggca gtcggctccc tcgttgaccg aatcaccgac
     8101 ctctctcccc agggggtacc accatggcca agcctttgtc tcaagaagaa tccaccctca
     8161 ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca
     8221 gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg
     8281 ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc
     8341 tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt
     8401 gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg
     8461 gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggcc
     8521 tgcagctgca gtagtaagaa ttctagatct tgagacaaat ggcagtattc atccacaatt
     8581 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     8641 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     8701 tttattacag ggacagcaga gatccacttt ggcgccggct cgaggggg
//
