LOCUS       Exported                 981 bp ds-DNA     linear   SYN 26-MAY-2021
DEFINITION  Mammalian expression of SLC8B1.
ACCESSION   .
VERSION     .
KEYWORDS    Human NCKX6-FlagTag (pcDNA3.1+)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Cai X, Lytton J
  TITLE     Molecular cloning of a sixth member of the K+-dependent Na+/Ca2+ 
            exchanger gene family, NCKX6.
  JOURNAL   J Biol Chem. 2004 Feb 13;279(7):5867-76. Epub 2003 Nov 18.
  PUBMED    14625281
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 26, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..981
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..366
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    732..745
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
ORIGIN
        1 cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag ctggctagcg tttaaactta agcttgatat ggccggcaga
      121 aggctgaatc tgcgctgggc actgagtgtg ctttgtgtgc tgctaatggc ggagacagtg
      181 tctgggacta ggggctcgtc tacaggagct cacattagcc cccagtttcc agcttcaggt
      241 gtgaaccaga cccccgtggt agactgccgc aaggtgtgtg gcctgaatgt ctctgaccgc
      301 tgtgacttca tccggaccaa ccctgactgc cacagtgatg gggactacaa ggacgacgat
      361 gacaaggggt acctggacta cctggaaggc atcttctgcc acttccctcc cagcctcctc
      421 cctctggctg tcactctcta cgtttcctgg ctgctctacc tgtttctgat tctgggagtc
      481 accgcagcca agtttttctg ccccaacttg tcggccattt ctaccacact gaagctctcc
      541 cacaacgtgg caggcgtcac cttcctggca tttgggaatg gtgcacctga catcttcagt
      601 gccctggtgg ccttctctga cccgcacaca gccggcctgg cccttggggc actgtttggc
      661 gctggcgtgc tggttaccac agtggtggcc ggaggcatta ccatcctaca ccccttcatg
      721 gctgcctcca ggcccttctt cagggacatc gttttctaca tggtggctgt gttcctgacc
      781 ttcctcatgc tcttccgtgg cagggtcacc ctggcatggg ctctgggtta cctgggcttg
      841 tatgtgttct atgtggtcac tgtgattctc tgcacctgga tctaccaacg gcaacggaga
      901 ggatctctgt tctgccccat gccagttact ccagagatcc tctcagactc cgaggaggac
      961 cgggtatctt ctaataccaa c
//
