LOCUS Exported 7399 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pACAGW-oChiEFtdTomato-AAV SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7399) AUTHORS Chris W. D. Jurgens, Karen A. Bell, A. Rory McQuiston, William Guido. TITLE Optogenetic Stimulation of the Corticothalamic Pathway Affects Relay Cells and GABAergic Neurons Differently in the Mouse Visual Thalamus. JOURNAL PLoS ONE 7(9): e45717 REFERENCE 2 (bases 1 to 7399) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7399 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 38..167 /label=AAV2 ITR promoter 478..734 /label=chicken beta-actin promoter /note="contains a 21-bp deletion relative to the standard chicken beta-actin promoter" primer_bind 1013..1035 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" primer_bind 1097..1116 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" CDS 2248..3678 /codon_start=1 /product="tandem dimeric (pseudo-monomeric) derivative of DsRed (Shaner et al., 2004)" /label=tdTomato /note="mammalian codon-optimized" /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE RSEGRHHLFLYGMDELYK" intron 3692..3824 /label=chimeric intron /note="chimera between introns from human beta-globin and immunoglobulin heavy chain genes" primer_bind 3807..3826 /label=pMT2-F /note="Synthetic intron, forward primer" misc_feature 3880..4468 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(3933..3953) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(4351..4362) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 4511..4632 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4522..4541) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 4576..4595 /label=SV40pA-R /note="SV40 polyA, reverse primer" repeat_region 4794..4923 /label=AAV2 ITR primer_bind complement(4989..5006) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(5160..5748) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5240..5259) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5919..6779) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6542..6561 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6780..6884) /gene="bla" /label=AmpR promoter rep_origin complement(6910..7365) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7047..7068) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 7259..7278 /label=F1ori-R /note="F1 origin, reverse primer" ORIGIN 1 agggcgatcg gtgcgggcct cttcgctatt acgccagctg cgcgctcgct cgctcactga 61 ggccgcccgg gcaaagcccg ggcgtcgggc gacctttggt cgcccggcct cagtgagcga 121 gcgagcgcgc agagagggag tggccaactc catcactagg ggttccttgt agttaatgat 181 taacccgcca tgctacttat ctacgtagcc atgctctagg aagagtacca ttgacgtcaa 241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg 301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc 361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct 421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg 481 aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt 541 tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggggc 601 ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg 661 cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa 721 gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg 781 cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg 841 acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc 901 tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct 961 cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc 1021 ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt 1081 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa 1141 ttggatcggg atccaccggt gaccatggtg agcagaagac cctggctgct ggccctggcc 1201 ctggccgtgg ccctggccgc cggcagcgcc ggcgccagca ccggcagcga cgccaccgtg 1261 cccgtggcca cccaggacgg ccccgactac gtgttccaca gagcccacga gagaatgctg 1321 ttccagacca gctacaccct ggagaacaac ggcagcgtga tctgcatccc caacaacggc 1381 cagtgcttct gcctggcctg gctgaagagt aacggcacca acgccgagaa gctggccgcc 1441 aacatcctgc agtggatcac cttcgccctg agcgccctgt gcctgatgtt ctacggctac 1501 cagacctgga agagtacctg cggctgggag gagatctacg tggccaccat cgagatgatc 1561 aagttcatca tagagtactt ccacgagttc gacgagcccg ccgtgatcta cagcagcaac 1621 ggcaacaaga ccgtgtggct gagatacgcc gagtggctgc tgacctgccc cgtggtcctg 1681 atccacctga gcaacctgac cggcctggcc aacgactaca acaagagaac catgggcctg 1741 ctggtgagcg acatcggcac catcgtgtgg ggcaccaccg ccgccctgag caagggctac 1801 gtgagagtga tcttcttcct gatgggcctg tgctacggca tctacacctt cttcaacgcc 1861 gccaaggtgt acatcgaggc ctaccacacc gtgcccaagg gcagatgcag acaggtggtg 1921 accggcatgg cctggctgtt cttcgtgagc tggggcatgt tccccatcct gttcatcctg 1981 ggccccgagg gcttcggcgt gctgagcgtg tacggcagca ccgtgggcca caccatcatc 2041 gacctgatga gcaagaactg ctggggcctg ctgggccact acctgagagt gctgatccac 2101 gagcacatcc tgatccacgg cgacatcaga aagaccacca agctgaacat cggcggcacc 2161 gagatcgagg tggagaccct ggtggaggac gaggccgagg ccggcgccgt gaacaagggc 2221 accggcaagt acgagagcag catcgatatg gtgagcaagg gcgaggaggt catcaaagag 2281 ttcatgcgct tcaaggtgcg catggagggc tccatgaacg gccacgagtt cgagatcgag 2341 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag 2401 ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttcatgta cggctccaag 2461 gcgtacgtga agcaccccgc cgacatcccc gattacaaga agctgtcctt ccccgagggc 2521 ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc tggtgaccgt gacccaggac 2581 tcctccctgc aggacggcac gctgatctac aaggtgaaga tgcgcggcac caacttcccc 2641 cccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccac cgagcgcctg 2701 tacccccgcg acggcgtgct gaagggcgag atccaccagg ccctgaagct gaaggacggc 2761 ggccactacc tggtggagtt caagaccatc tacatggcca agaagcccgt gcaactgccc 2821 ggctactact acgtggacac caagctggac atcacctccc acaacgagga ctacaccatc 2881 gtggaacagt acgagcgctc cgagggccgc caccacctgt tcctggggca tggcaccggc 2941 agcaccggca gcggcagctc cggcaccgcc tcctccgagg acaacaacat ggccgtcatc 3001 aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca cgagttcgag 3061 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg 3121 accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt catgtacggc 3181 tccaaggcgt acgtgaagca ccccgccgac atccccgatt acaagaagct gtccttcccc 3241 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt gaccgtgacc 3301 caggactcct ccctgcagga cggcacgctg atctacaagg tgaagatgcg cggcaccaac 3361 ttcccccccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccaccgag 3421 cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc accaggccct gaagctgaag 3481 gacggcggcc actacctggt ggagttcaag accatctaca tggccaagaa gcccgtgcaa 3541 ctgcccggct actactacgt ggacaccaag ctggacatca cctcccacaa cgaggactac 3601 accatcgtgg aacagtacga gcgctccgag ggccgccacc acctgttcct gtacggcatg 3661 gacgagctgt acaagtagct cgaggccgca ggtaagtatc aaggttacaa gacaggttta 3721 aggagaccaa tagaaactgg gcttgtcgag acagagaaga ctcttgcgtt tctgataggc 3781 acctattggt cttactgaca tccactttgc ctttctctcc acaggtgtcg agtggagctc 3841 gcgactagtc gattcgaatt cgatatcaag cttatcgata atcaacctct ggattacaaa 3901 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac 3961 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc 4021 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt 4081 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc 4141 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc 4201 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg 4261 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt 4321 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc 4381 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt 4441 cggatctccc tttgggccgc ctccccgcat cgataccgtc gacccgggcg gccgcttcga 4501 gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa 4561 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc 4621 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg 4681 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatctt 4741 cctagagcat ggctacgtag ataagtagca tggcgggtta atcattaact acaaggaacc 4801 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg 4861 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg 4921 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 4981 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 5041 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 5101 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 5161 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 5221 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 5281 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 5341 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 5401 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 5461 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 5521 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 5581 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc 5641 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 5701 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 5761 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 5821 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 5881 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 5941 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 6001 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 6061 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 6121 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 6181 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 6241 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 6301 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 6361 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 6421 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 6481 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 6541 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 6601 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 6661 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 6721 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 6781 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 6841 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 6901 gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa 6961 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat 7021 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg 7081 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac 7141 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta 7201 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag 7261 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg 7321 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca 7381 ggctgcgcaa ctgttggga //