LOCUS Exported 989 bp ds-DNA linear SYN 17-MAY-2021 DEFINITION 3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259). ACCESSION . VERSION . KEYWORDS pRSV-Rev SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 989) AUTHORS Dull T, Zufferey R, Kelly M, Mandel RJ, Nguyen M, Trono D, Naldini L TITLE A third-generation lentivirus vector with a conditional packaging system. JOURNAL J Virol. 1998 Nov . 72(11):8463-71. PUBMED 9765382 REFERENCE 2 (bases 1 to 989) AUTHORS . TITLE Direct Submission JOURNAL Exported May 17, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..989 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 216..233 /label=L4440 /note="L4440 vector, forward primer" protein_bind 350..371 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 386..416 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 424..440 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 429..451 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 448..464 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 448..464 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" promoter 609..870 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" primer_bind 881..905 /label=LNCX /note="Human CMV promoter, forward primer" ORIGIN 1 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 61 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 121 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag 181 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg 241 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct 301 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt 361 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg 421 gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt acgaattcga 481 tgtacgggcc agatatacgc gtatctgagg ggactagggt gtgtttaggc gaaaagcggg 541 gcttcggttg tacgcggtta ggagtcccct caggatatag tagtttcgct tttgcatagg 601 gagggggaaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt 661 tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg 721 tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc 781 actgaattcc gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacgcc 841 atttgaccat tcaccacatt ggtgtgcacc tccaagctcg agctcgttta gtgaaccgtc 901 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat 961 ccagcctccc ctcgaagcta gtcgattag //