LOCUS Exported 3210 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Expresses shRNA against PICK1. ACCESSION . VERSION . KEYWORDS pSuper-PICK1 shRNA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3210) AUTHORS Anggono V, Clem RL, Huganir RL TITLE PICK1 loss of function occludes homeostatic synaptic scaling. JOURNAL J Neurosci. 2011 Feb 9;31(6):2188-96. doi: 10.1523/JNEUROSCI.5633-10.2011. PUBMED 21307255 REFERENCE 2 (bases 1 to 3210) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3210 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin complement(3..458) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(140..161) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 352..371 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 585..607 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 599..616 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 600..616 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 623..642 /label=T7 /note="T7 promoter, forward primer" promoter 623..641 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind 674..690 /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind 674..690 /label=pBluescriptSK /note="For pBluescript vector" promoter 710..924 /label=H1 promoter /note="human H1 RNA promoter" primer_bind 845..864 /label=H1 /note="Human H1 promoter, forward primer" promoter complement(1022..1040) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(1061..1077) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1061..1077) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1074..1096) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 1085..1101 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1109..1139) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 1154..1175 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(1292..1309) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1463..2051) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1543..1562) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(2222..3082) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2845..2864 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(3083..3187) /gene="bla" /label=AmpR promoter ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg 481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg agctccaccg 661 cggtggcggc cgctctagaa ctagtggatc ccccgggctg caggaattcg aacgctgacg 721 tcatcaaccc gctccaagga atcgcgggcc cagtgtcact aggcgggaac acccagcgcg 781 cgtgcgccct ggcaggaaga tggctgtgag ggacagggga gtggcgccct gcaatatttg 841 catgtcgcta tgtgttctgg gaaatcacca taaacgtgaa atgtctttgg atttgggaat 901 cttataagtt ctgtatgaga ccacagatcc ccgcctcacc atcaagaagt accttcaaga 961 gaggtacttc ttgatggtga ggcttttttc tcgagggggg gcccggtacc agcttttgtt 1021 ccctttagtg agggttaatt tcgagcttgg cgtaatcatg gtcatagctg tttcctgtgt 1081 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 1141 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 1201 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 1261 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 1321 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 1381 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 1441 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 1501 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 1561 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 1621 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 1681 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 1741 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 1801 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 1861 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct 1921 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 1981 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 2041 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 2101 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 2161 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 2221 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 2281 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 2341 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 2401 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 2461 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 2521 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 2581 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 2641 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 2701 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 2761 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 2821 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 2881 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 2941 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 3001 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 3061 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 3121 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 3181 ttccgcgcac atttccccga aaagtgccac //