LOCUS Exported 687 bp ds-DNA linear SYN 17-MAY-2021 DEFINITION Co-expresses human optimized S. pyogenes dCas9 and GFP. ACCESSION . VERSION . KEYWORDS pLV hUbC-dCas9-T2A-GFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 687) AUTHORS Kabadi AM, Ousterout DG, Hilton IB, Gersbach CA TITLE Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector. JOURNAL Nucleic Acids Res. 2014 Aug 13. pii: gku749. PUBMED 25122746 REFERENCE 2 (bases 1 to 687) AUTHORS . TITLE Direct Submission JOURNAL Exported May 17, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..687 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(17..70) /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS complement(77..97) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" ORIGIN 1 ccttgctcac ggtaccaggg ccgggattct cctccacgtc accgcatgtt agaagacttc 61 ctctgccctc gctagccacc ttcctcttct tcttggggtc agccctgctg tctccaccga 121 gctgagagag gtcgattctt gtttcataga gccccgtaat tgactgatga atcagtgtgg 181 cgtccaggac ctcctttgta gaggtgtacc gctttctgtc tatggtggtg tcgaagtact 241 tgaaggctgc aggcgcgccc aagttggtca gagtaaacaa gtggataatg ttttctgcct 301 gctccctgat gggcttatcc ctgtgcttat tgtaagcaga aagcacctta tcgaggttag 361 cgtcggcgag gatcactctt ttggagaatt cgcttatttg ctcgatgatc tcatcaaggt 421 agtgtttgtg ttgttccacg aacagctgct tctgctcatt atcttcggga gaccctttga 481 gcttttcata gtggctggcc agatacaaga aattaacgta tttagagggc agtgccagct 541 cgttaccttt ctgcagctcg cccgcactag cgagcattcg tttccggccg ttttcaagct 601 caaagagaga gtacttggga agcttaatga tgaggtcttt tttgacctct ttatatcctt 661 tcgcctcgag aaagtcgatg gggtttt //